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Citations for Bioline :
51 - 100 of 936 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Bacterial cultures were grown in Luria Bertani (LB) broth for 18-24h at 37 °C and DNA was extracted using the Isolate II Genomic DNA Kit (Bioline, UK) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After selection on LB-agar kanamycin plates the resulting colonies were first screened by colony PCR using MyTaq DNA polymerase (Bioline) to check for the presence of the right size insert using T7forward and T7reverese primers ...
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bioRxiv - Genetics 2022Quote: ... The Endpoint PCR amplification (gDNA concentration of 20-50 ng μl−1 per sample) for each target region were performed using MyFi™ DNA Polymerase Kit (Bioline GmbH, Luckenwalde, Germany) and specific primers (IDENTXX GmbH)in a PCR thermal cycler (T100 PCR thermal cycler ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was extracted using the ISOLATE II RNA/DNA/Protein kit (Bioline, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of knockout mutants was isolated using the ISOLATE II kit (Bioline). Genomic DNA was cut using KpnI (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: RNA was isolated using Bioline II DNA/RNA/Protein extraction kit (Bioline, Australia) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The samples were collected 72 hours after the addition of the drugs and genomic DNA (gDNA) was extracted using the ISOLATE II genomic DNA kit (Bioline, BIO-52067). The samples were then quantified using Nanodrop and 200 ng of gDNA was used in the library PCRs that were performed as for the screening above ...
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bioRxiv - Immunology 2024Quote: Parasite genomic DNA was isolated from mouse peritoneal exudate cells and whole brain using the Isolate II Genomic DNA Kit (Bioline, BIO-52067). Prior to isolation ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA samples were prepared using the SensiFAST SYBR Lo-Rox kit (Bioline, BIO-94020), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were prepared using the MyTaq DNA polymerase kit (Bioline, Cat# BIO-21111). Reactions contained a 5× MyTaq Buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized with the SensiFAST cDNA Synthesis Kit (Bioline; Cat. No. 65054). Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline ...
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bioRxiv - Plant Biology 2023Quote: Measurements were carried out in 96-well plates using the SensiFAST™ SYBR® Kit (Bioline, Luckenwalde, Germany) in a LightCycler® 480 (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... we used the ISOLATE II RNA mini kit including on-column DNA digestion (Bioline, UK). The concentrations were measured by NanoDrop ND-1000 (Peqlab ...
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bioRxiv - Physiology 2022Quote: ... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions of all DNA samples were performed using the SensiFAST SYBR No-ROX Kit (Bioline) on a Mic (Magnetic Induction Cycler ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μl of 1:10 diluted cDNA and BIOTAQ™ DNA polymerase (Bioline GmbH) were used in a CFX96™ Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 1:1 diluted cDNA and SensiFAST SYBR No-ROX Kit (#BIO-98005, Bioline). Knockdown was assessed using ΔΔCt analysis as described by Pfaffl [92].
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bioRxiv - Cancer Biology 2022Quote: ... The purified DNAs were amplified with SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) by real-time qPCR.
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The size and quality of extracted DNA was checked by electrophoresis in 1% agarose gel (Bioline) including GelRed™ (Biotium) ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase ...
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bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was suspended in 100 μl of preheated elution buffer G (ISOLATE II Genomic DNA kit, Bioline Meridian). The DNA quality and quantity was checked by Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 4.2 µL purified DNA from chromatin immunoprecipitation was mixed with 5 µL 2x SensiFAST SYBR No-ROX kit (Bioline) and 0.4 µL forward and reverse primer (10 µM) ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA extraction from tail snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). All the mice were genotyped by the fragment PCR method as indicated in the Jackson Laboratory website ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified RNA was then reverse transcribed into complementary DNA (cDNA) with the SensiFAST™ complementary cDNA synthesis Kit (Bioline) and used in qPCR ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA extraction from tails snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). Mice were genotyped using a fragment PCR method with the primers shown in the table below and indicated on the Jackson Laboratory website ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using 2 µg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Complementary DNA was synthesized using 2 μg of RNA and the Tetro cDNA synthesis kit (Bioline Reagents Ltd., London, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 500-ng portions of DNA extracted from each larva were subjected to qPCR using the SensiMic SybR Green kit (Bioline). Forward (5′-ACTTCCGCAATGGACGTTAC-3′ ...
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bioRxiv - Cell Biology 2023Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) ...
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bioRxiv - Neuroscience 2024Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline ...
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bioRxiv - Plant Biology 2020Quote: ... ‘Kitaake’ using a high fidelity Taq DNA polymerase (Bioline, VELOCITY DNA Polymerase) and cloned into entry vector pDONR221 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... Agarose gels (1.5%) were prepared in 1 X TAE buffer (Astral Scientific) with 0.01% SYBR Safe DNA gel stain (Bioline). PCR products (5 µL ...
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bioRxiv - Microbiology 2024Quote: ... 1 ng/μl of total DNA was amplified using the primer sets in SensiFAST SYBR No-ROX (Bioline). qPCR was realized in technical duplicates in the Rotor-Gene Q real-time PCR cycler (Qiagen) ...
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bioRxiv - Pathology 2020Quote: Wheat curl mite DNA was extracted from single mites using the MyTaq™ Extract-PCR Kit (Bioline Meridian Bioscience, London, UK)) according to the manufacturer’s recommendations with the exception of the amount of starting material being less than 3 mg ...
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bioRxiv - Biochemistry 2022Quote: ... RNA samples (1 µg) were reverse transcribed using SensiFAST cDNA synthesis kit (Bioline) according to manufacturer’s protocol and subjected to Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Complementary DNA (cDNA) used for in situ hybridisation and quantitative PCR (qPCR) was synthesised using the Tetro cDNA synthesis kit (Bioline, London, UK) with oligo-dT primers according to the manufacturers’ instructions.
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the NucliSENS® EasyMag® (BioMérieux, Marcy l’Etoile, France) or the Isolate II Genomic DNA kit (Bioline, Tennessee, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the qPCR analysis was performed on each DNA sample in duplicates by using SensiFAST™ SYBR® No-ROX Kit (Bioline, UK) and primer sets summarised in table 2 at a final concentration of 1 pM of each primer ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from whole female BF post adult and pupal injection using an Isolate II Genomic DNA extraction kit (Bioline, NSW, Australia). Six flies were assayed at each point of time for determination of the relative Wolbachia density ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.4 μM zebrafish genotyping primers (Extended Data Fig.5A, Table 2) and 1 x MyTaq Red DNA Polymerase (Bioline) according to the manufacturer’s protocol in a T100 thermal cycler ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of each forward primer 341F 5’-NNNNNNNNNNTCCTACGGGNGGCWGCAG and reverse primer 785R 5’-NNNNNNNNNNTGACTACHVGGGTATCTAAKCC in 20 μL volume of 1 x MyTaq buffer containing 1.5 units MyTaq DNA polymerase (Bioline) and 2 μl of BioStabII PCR Enhancer (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... 1 μl forward and reverse primers (10 mM) and 0.5 μl (5 U/μl) BioTaq DNA polymerase (Bioline, London) and amplified using PCR thermal cycler (BiometraTOne ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCRs were run using 2 µl cDNA (diluted 1:1) in a 10 µl reaction with 5 µl SensiFAST SBYR No-ROX Kit (Bioline) and 0.1 µM of each primer ...
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bioRxiv - Bioengineering 2021Quote: ... and Velocity DNA polymerase (Bioline) were used according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... 0.25μl MyTaq DNA polymerase (Bioline) and 5μl DNA template ...
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bioRxiv - Plant Biology 2021Quote: ... MyRed Taq DNA polymerase (Bioline) was used in a reaction volume of 20 μl ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s recommendations ...