Labshake search
Citations for Bioline :
151 - 200 of 247 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 1x MyTaq Red Reaction buffer red and 0.5 μl of MyTaq DNA polymerase (Bioline) on SimpliAmp thermal cycler (Applied Biosystems ...
-
bioRxiv - Genomics 2023Quote: ... PCR reactions were prepared using the MyTaq DNA polymerase kit (Bioline, Cat# BIO-21111). Reactions contained a 5× MyTaq Buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized with the SensiFAST cDNA Synthesis Kit (Bioline; Cat. No. 65054). Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline ...
-
bioRxiv - Molecular Biology 2024Quote: The library preparation was performed as follows: genomic DNA was isolated (Bioline, bio-52067) and ∼ 500 ng were digested with DpnI (10 U ...
-
bioRxiv - Molecular Biology 2024Quote: DNA was extracted from lung tissue and digested using proteinase K (Bioline: BIO-37084) for genomic excision gels ...
-
bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
-
bioRxiv - Genomics 2020Quote: ... but without gel purification and with use of the Velocity DNA Polymerase (BioLine, Luckenwalde, Germany) for 25 cycles of amplification ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were carried out with ∼100 ng genomic DNA in MyTaq Red mix (Bioline) and purified using the PCR Isolate II PCR and Gel Kit (Bioline ...
-
bioRxiv - Microbiology 2021Quote: ... but without gel purification and with use of the Velocity DNA Polymerase (BioLine, Luckenwalde, Germany) for 25 cycles of amplification ...
-
bioRxiv - Genetics 2020Quote: ... but without gel purification and with use of the Velocity DNA Polymerase (BioLine, Luckenwalde, Germany) for 12 cycles of amplification for the FLAG-CenH3 ...
-
bioRxiv - Cell Biology 2021Quote: ... and all the used strains were genotyped before its use using MyTaqTM DNA polymerase (Bioline) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: Two versions of arsB were PCR amplified using VELOCITY DNA Polymerase (Bioline; catalog #BIO-21098) following manufactures recommendations ...
-
bioRxiv - Neuroscience 2024Quote: ... we used the ISOLATE II RNA mini kit including on-column DNA digestion (Bioline, UK). The concentrations were measured by NanoDrop ND-1000 (Peqlab ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1x MyTaq™ reaction buffer and 1.0 U of MyTaq™ DNA polymerase (Bioline Meridian Bioscience) adjusted to a total volume of 20µL ...
-
bioRxiv - Developmental Biology 2022Quote: ... One microlitre of Chelex extracted DNA was amplified with the appropriate primers and MyTaq polymerase (Bioline) in a 20 μl volume ...
-
bioRxiv - Microbiology 2019Quote: ... The size and quality of extracted DNA was checked by electrophoresis in 1% agarose gel (Bioline) including GelRed™ (Biotium) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The obtained gDNA was used for amplification of the register module using MyTaqTM DNA Polymerase (Bioline) and a pair of specific primers for the PB or RL configuration and the PhiC31 recombinase (Table S2) ...
-
bioRxiv - Genomics 2019Quote: ... pool C was diluted 1,000-fold and 1.5 fmol were amplified using VELOCITY DNA polymerase (Bioline) with forward primer pool-FP1 and reverse primer pool-RP2 using the following PCR conditions ...
-
bioRxiv - Neuroscience 2020Quote: ... target cDNA sequences were amplified for 35 reaction cycles with the enzyme MyTaq DNA Polymerase (Bioline). The cDNA of the housekeeping gene ATP5O (ATP synthase ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The tagmented DNA was added to a PCR reaction with 2x MyTaq mix (BIO-25041; Bioline) with primers that add library specific indexes and amplified for 10 cycles ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA was precipitated from fractions by adding 4μl of Co-precipitant Pink Linear Polyacrylamide (Bioline) and 2 volumes of PEG-NaCl solution (30% w/v polyethylene glycol 6000 ...
-
bioRxiv - Genomics 2022Quote: ... TE-genome junction validation PCRs were performed using MyTaq HS DNA Polymerase (Bioline, Cat#: BIO-2111). Reaction mixes contained 5μL 5× MyTaq Reaction Buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... target regions were amplified by PCR using 50 ng genomic DNA in MyTaq Red mix (Bioline) according to manufacture instructions (primers are shown in Table S3) ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR reactions of all DNA samples were performed using the SensiFAST SYBR No-ROX Kit (Bioline) on a Mic (Magnetic Induction Cycler ...
-
bioRxiv - Plant Biology 2023Quote: The subsequent amplification step of the PCR was performed using MangoTaq™DNA Polymerase (Bioline, Belgium) and the primers LCO1490 and HCO2198 designed by Folmer et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Vectors for mutant nsp14 expression were generated by QuikChange site-directed mutagenesis using Accuzyme DNA polymerase (Bioline) and verified by sequence analysis ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were diluted and 5 μl was used for genotyping PCR using MyTaq DNA polymerase (Bioline) with indicated DNA primers ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were generated by PCR amplification of sequences of interest with Velocity DNA Polymerase (Bioline, #BIO-21099) using primers synthesized by IDT or restriction enzyme digestion with NEB High-Fidelity enzymes ...
-
bioRxiv - Microbiology 2024Quote: ... 4.2 µL of extracted DNA was combined with 5 µL of MyTaq Red polymerase (Bioline, Meridian Bioscience), 0.4 µL of 10 uM forward primer (nexF-N3-6-515f for 16S or nexF-N3-6-ITS1f-KYO1 for ITS) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The purified DNAs were amplified with SYBR Green SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) by real-time qPCR.
-
bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted using alkaline lysis after which the EntF* fragment was amplified using 2x Biomix (Bioline, Belgium) in a Mastercycler PCR system (Eppendorf ...
-
bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... The full-length genome or the nsp14-coding region were amplified by PCR using MyTaq DNA polymerase (Bioline) and after purification the PCR product was sequenced by Sanger sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... Each sample contained 2μl of boiled cell solution and 18μl of the PCR master mix with MyTaq DNA Polymerase (Bioline). The PCR cycling conditions were 95 ᵒC 5 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... 20 ng DNA was used in a 10 μl reaction containing 1x Sensimix Sybr Hi-Rox reagents (Bioline) and 375 nM of both forward and reverse primer (Supplementary Table S1) ...
-
bioRxiv - Microbiology 2020Quote: ... MERS-CoV nsp14 mutant expression plasmids were generated by Quikchange site-directed mutagenesis using Accuzyme DNA polymerase (Bioline) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Agarose gels (1.5%) were prepared in 1 X TAE buffer (Astral Scientific) with 0.01% SYBR Safe DNA gel stain (Bioline). PCR products (5 µL ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR products were size separated by gel electrophoresis on a gel composed of 2% DNA grade agarose (Bioline) in TAE buffer (40 mM Tris Acetate ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 600 ng of isolated RNA was reverse transcribed to complementary DNA (cDNA) with SensiFAST cDNA Synthesis -kit (Bioline) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... 1 ng/μl of total DNA was amplified using the primer sets in SensiFAST SYBR No-ROX (Bioline). qPCR was realized in technical duplicates in the Rotor-Gene Q real-time PCR cycler (Qiagen) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were carried out in a volume of 15 μL including 0.25-1.0 U of MyTaq™ HS DNA polymerase (Bioline), 2 μL of MyTaq™ 5x reaction buffer (Bioline) ...
-
bioRxiv - Neuroscience 2019Quote: ... Amplified products were visualized with reference to DNA ladders (either: Hyperladder I, Bioline, London, UK, or 1kb ladder, Promega), using GeneSnap (Syngene ...
-
bioRxiv - Developmental Biology 2019Quote: ... 0.4 μM zebrafish genotyping primers (Extended Data Fig.5A, Table 2) and 1 x MyTaq Red DNA Polymerase (Bioline) according to the manufacturer’s protocol in a T100 thermal cycler ...
-
bioRxiv - Molecular Biology 2020Quote: ... The genomic regions flanking the nuclease target sites were PCR amplified using MyTaq™ DNA Polymerase (Bioline, https://www.bioline.com/) and primers listed on Table S3 ...
-
bioRxiv - Microbiology 2021Quote: ... 2μL were used as template for genomic PCRs in a 50 μL reaction with Velocity DNA Polymerase (Bioline#21098) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of each forward primer 341F 5’-NNNNNNNNNNTCCTACGGGNGGCWGCAG and reverse primer 785R 5’-NNNNNNNNNNTGACTACHVGGGTATCTAAKCC in 20 μL volume of 1 x MyTaq buffer containing 1.5 units MyTaq DNA polymerase (Bioline) and 2 μl of BioStabII PCR Enhancer (Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... SureClean was applied to purify DNA or vector between steps as described by the manufacturer (Bioline GmbH, Luckenwalde, Germany). Finally ...
-
bioRxiv - Molecular Biology 2020Quote: ... A CCDC26 exon 6 DNA template was generated by performing a PCR using Red Mix (Bioline, Cat. No. BIO25043) WT K562 cDNA and CCDC26-specific primers that incorporated a T7 RNA polymerase promoter at the 5’-end of the DNA strand to be later transcribed (Supplementary Table 1) ...
-
bioRxiv - Genetics 2020Quote: ... Genotyping was performed by PCR of genomic tail DNA using the Extract PCR kit (Bioline, cat. No. BIO-21127). All mice were kept on C57BL/6J (The Jackson Laboratory ...
-
bioRxiv - Genetics 2020Quote: ... 1 μl forward and reverse primers (10 mM) and 0.5 μl (5 U/μl) BioTaq DNA polymerase (Bioline, London) and amplified using PCR thermal cycler (BiometraTOne ...