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Citations for Bioline :
151 - 200 of 248 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Target gene real-time qPCR analyses were conducted using SYBR Green Master Mix (Bioline #BIO-98005) with the LightCycler® 480 System (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Real time qPCR was performed using a RotorGene 6000 (Corbett Research) instrument with SYBR (Bioline, UK) and results were analysed using beta-actin (Actb ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Genetics 2023Quote: ... The RT-qPCR reactions were set up using SensiFAST SYBR No-ROX kit (Bioline, BIO-98005) in a Light Cycler 480 II system (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020). cFos primers were from9 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qPCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR reactions were carried out using PyroTaq EvaGreen mix Plus (ROX; CulteK Molecular Bioline, Madrid, Spain) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020). For sequencing library preparation ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcriptase quantitative PCR (RT-qPCR) reactions were performed with the SensiFast SYBR Green Mastermix (Bioline) on a Bio-RAD cfx96 machine ...
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bioRxiv - Genetics 2021Quote: ... Primers flanking the MT-ND4 gene were used to amplify the edited locus using MyTaq polymerase (Bioline, USA). PCR amplicons were gel extracted and purified using QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 1 ng/μl of total DNA was amplified using the primer sets in SensiFAST SYBR No-ROX (Bioline). qPCR was realized in technical duplicates in the Rotor-Gene Q real-time PCR cycler (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... primers at a final concentration of 800nM and 6μL of SensiFAST™ SYBR® Lo-ROX Kit (Bioline). qPCR conditions were ...
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bioRxiv - Systems Biology 2020Quote: ... Naïve and primed marker expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline). Primers are listed in Supplementary Table 6.
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR or RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers on a Applied Bioscience StepOnePlus qPCR machine (Life Technologies) ...
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bioRxiv - Genetics 2021Quote: Quantitative polymerase chain reaction (qPCR) was performed using SensiFAST™ SYBR No-ROX Kit (Bioline, BIO-98020) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed with 500ng of cDNA and the SensiFast™ SYBR® No-ROX kit (Bioline) and a Lightcycler® 480/1536 (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using SensiFAST™ SYBR® Hi-ROX Kit (Bioline, cat. no. BIO-92020) in a 96-well white plate (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... All testing samples were used in qPCR according to SensiFASTTM SYBR® No-ROX Kit manual (Bioline). NvDsx qPCR primers were designed on the common region (exon 2-5 ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 30-fold and 5μl were used for qPCR using SensiMix SYBR low-ROX (Bioline) with 150nM Forward and Reverse primers (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR reactions were setup using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2µM ...
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bioRxiv - Genetics 2020Quote: ... 1 μl forward and reverse primers (10 mM) and 0.5 μl (5 U/μl) BioTaq DNA polymerase (Bioline, London) and amplified using PCR thermal cycler (BiometraTOne ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized utilizing a gene-specific primer designed to target the 10xSunTag socRNA and Tetro Reverse Transcriptase (Bioline). The resulting cDNA was isolated via column-based purification using the GeneJet Gel Extraction Kit (Thermo Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was performed directly from the lysates with region targeting primers (Supplementary Table 2) and MyTaq Red Mix (Bioline) followed by gel electrophoresis to reveal biallelic knockout clones ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions and first strand cDNA synthesis was performed with oligo dT primers using BioScript reverse transcriptase (Bioline). GdMYBSG6 sequences have been deposited in GenBank under the following accession numbers ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 μM final concentration of sense and antisense primer (see Table S1 for sequences) and MyTaq Red Mix (Bioline) was used ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well plates were loaded with 15μl reaction mixture (1:25 primers, 1:3 cDNA and 1:2 SYBR-Green SensiMix (Bioline)) ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR reactions were performed in triplicate on 5ng of cDNA using SensiFAST SYBR No-ROX (Bioline #BIO-98005) on a LightCycler 96 (Roche Life Science) ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with 50 ng of cDNA in each well and SensiFAST probe no-ROX mix (Bioline) using a CFX Connect system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1μL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Genetics 2022Quote: The relative amount of PCR product was quantified by qPCR using SensiFASTTM SYBR® Hi -ROX Kit (Bioline). The sequences of primers used for the quantitative detection of the chromosomal loci are listed in Suppl Table ST6 ...
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bioRxiv - Microbiology 2022Quote: ... conventional PCR assays were replaced with qPCR assays with SensiFAST™ SYBR® No-ROX Kit (Bioline, Australia). The cycling conditions included an initial denaturation at 95°C for 3 min followed by 28 -40 cycles of 95°C for 5 sec ...
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bioRxiv - Genetics 2024Quote: ... The qPCR was performed using a 20 µl reaction containing 2X SensiFAST SYBR No-ROX master mix (Bioline), 10 µM of primers and diluted DNA (1:50 dilution in nuclease free water ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was done using gene-specific primers (see Supplemental Table 1) in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to an ACTIN control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lyrata PER plants as above and a short section of ASY3 was PCR amplified using 0.5 μM primers (S5 Table) and MyTaq™ Red Mix (Bioline). PCR conditions were as follows ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were generated from 200 ng genomic DNA (gDNA) using junction-specific primers (Table S4) by a two-step nested PCR with Velocity polymerase (Bioline). The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1) for each locus using My Taq HS mix (Bioline) and gel purification ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was done using gene-specific primers in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to Actin control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR product was run on a 1% agarose gel cut out to remove remaining primer dimers and cleaned with PCR Isolate II PCR and Gel Kit (Bioline). The purified libraries were sequenced on an Illumina HiSeq2500 or MiSeq depending on the expected complexity of the library.
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μL of DNA was used in a PCR reaction using 0.3 μΜ primers and 5U of MyTaq DNA polymerase (Bioline).
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bioRxiv - Cell Biology 2020Quote: Real-Time PCR analysis was performed using TaqMan gene expression assays using primers (see Resources Table) and SensiFast Probe Lo-ROX Kit (cat.# BIO-84020, Bioline) following manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl of the dilution was mixed with 0.41 μM primers (Supplemental Table S4) and 1x Sensifast SYBR non-Rox kit (Bioline Bio98005). The real-time PCR program cycled as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP DNA and input DNA were diluted 5X and amplified using 500 nM primers and SensiFAST SYBR (No-ROX Kit, Bioline) using a LightCycler 480 (Roche).
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bioRxiv - Genetics 2022Quote: ... 1 μg of RNA was then reverse transcribed with random hexamer and anchored oligo dT primers using SensiFast cDNA synthesis kit (Bioline). Quantitative PCR was performed on the diluted cDNA using SensiFast probe (Bioline ...
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bioRxiv - Developmental Biology 2024Quote: ... and qPCR was performed using gene-specific primer sets (listed in Supplemental Table 1) and SensiFast SYBR Hi-ROX master mix (Bioline). MiRNA qRT-PCR was performed with the polyA and SYBR Green method as previously described using miRNA-specific forward primers and a 3’ RACE adaptor reverse primer62 ...