Labshake search
Citations for GE Life Sciences :
51 - 100 of 497 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
-
bioRxiv - Molecular Biology 2021Quote: ... CM were collected after 48h of transfection and filtered through 0.45 μm syringe filters and used for protein purification via ÄKTA pure 25 FPLC (GE Healthcare) equipped with 1-ml HisTrap affinity column (HisTrap HP ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Viral supernatant was harvested 48 h after transfection and filtered through a 0.45 μm polyethersulfone membrane filter (GE Healthcare Whatman). Western blotting of the viral supernatants with anti-V5 antibodies (below ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
-
bioRxiv - Cell Biology 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
-
bioRxiv - Biochemistry 2022Quote: ... Gels were scanned for fluorescence in a Typhoon Trio Scanner 9400 (Control v5.0 + variable Mode Imager-RA 501: PRT
-
bioRxiv - Bioengineering 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
-
bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
-
bioRxiv - Neuroscience 2023Quote: ... Knockdown experiments involved the use of Accell non-targeting control siRNA (D-001910-01, GE Healthcare Dharmacon, Lafayette, CO, USA) and Accell mouse Rbfox1 siRNA (A-041929-13 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Baseline and injection point alignment were performed and the control-corrected sensorgrams were analyzed using the BiaEvaluation software (GE Healthcare).
-
bioRxiv - Neuroscience 2022Quote: ... Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare). The infectivity titer of each virus (expressed as transduced units/ml ...
-
bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
-
bioRxiv - Neuroscience 2021Quote: ... and AAV2-CMV-mKO) were produced using a helper-free triple transfection procedure and purified via affinity chromatography (GE Healthcare, USA).
-
bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
-
bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
-
bioRxiv - Immunology 2021Quote: ... Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
-
bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
-
bioRxiv - Molecular Biology 2023Quote: ... The medium was changed one day after transfection and live cells were imaged on an IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification before the plate was returned to the incubator and cells were allowed to expand ...
-
bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
-
bioRxiv - Biochemistry 2022Quote: ... including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software; GE Healthcare). All measurements were performed with 2-fold serial dilutions using 60 s association followed by a dissociation time of more than 240 s at 25 °C with a flow rate of at 30 μl min−1 ...
-
bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
-
bioRxiv - Cancer Biology 2020Quote: ... Quality control of the purified products included SDS-PAGE and size exclusion chromatography using an Äkta Pure FPLC system (GE Healthcare) with a Superdex S200 10/300 increase column at a flow rate of 0.75 mL/min (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
-
bioRxiv - Microbiology 2023Quote: ... M-003854-01-0005) and a “NonTargeting” (SMARTpool siGENOME NonTargeting, D-001210-04-20) control siRNAs were obtained from GE Healthcare Dharmacon (Lafayette ...
-
bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-GST (GE Healthcare, Cat# 27457701), anti-HA (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were incubated for 3-5 days at 27 °C and transfection efficiency was monitored regularly by observing YFP expression level using a fluorescence microscope (DeltaVision, Applied Precision, LLC). At >50% transfection efficiency ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
-
bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences). After concentration ...
-
bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... 25nM siRNA were transfected in respective wells using 0.35µl of Dharmafect transfection reagent as per manufacturer’s instructions (GE Healthcare Dharmacon Inc. Co, USA). 12 hours post transfection of siRNA ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
-
bioRxiv - Genetics 2023Quote: ... The cultured cells were imaged live two days after transfection on an InCell Analyzer 6000 automated confocal fluorescence microscope (GE Healthcare Lifesciences).
-
bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
-
bioRxiv - Cell Biology 2021Quote: ... human ECs were transfected twice (at 0 and 24 h) with 200 pmol of siGENOME Non-Targeting siRNA #1 (as control) or siGENOME SMART pools siRNA oligonucleotides (GE Healthcare Dharmacon), using Oligofectamine Transfection Reagent (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... total RNA from 30–50 light-treated or dark control embryos was extracted using the illustra™ RNAspin Mini kit (GE Healthcare). 1 μg total RNA was used for reverse transcription with qScript XLT cDNA SuperMix (Quantabio) ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
-
bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
-
bioRxiv - Bioengineering 2019Quote: ... proteins in 2% SDS were bound to Paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) on filter plates (Multiscreen ...
-
bioRxiv - Biophysics 2019Quote: ... or acceptors (Cy5, GE Healthcare, Cat#PA15101) or EZ-Link™ NHS-Biotin (Thermo Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
-
bioRxiv - Cancer Biology 2021Quote: ... with 10% FBS (Cat# SV30160.03, GE Healthcare). Cells were monthly checked by qPCR (Venor® GeM qEP 11-9250 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 20nM siRNA against hsa-CHST15_0003 or hsa-TNFRSF21_0001 or a non-targeting siRNA control (GE Healthcare Dharmacon, Inc., Lafayette, CO, USA) using Lipofectamine 3000 (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... (USA). HyClone phosphate buffered saline (PBS, Cat. #SH30256.01) and RPMI medium modified (Cat. #SH30809.01) were purchased from GE Healthcare Life Sciences HyClone Laboratories (USA) ...