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Citations for GE Life Sciences :
1 - 50 of 497 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Transfection efficiency was determined using the fluorescently-labelled siGLO RISC-free control siRNA (GE Healthcare) and miRNA and/or mRNA expression was assessed afterwards.
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bioRxiv - Molecular Biology 2022Quote: ... siGENOME Control Pool Non-Targeting #2 (cat# D-001206-14-20) was purchased from GE Healthcare. siRNA transfection was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... The plasmid of Non-silencing Verified Negative Control (Cat # RHS4346) was used for the technical manual provided by GE Healthcare. negative control ...
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bioRxiv - Cell Biology 2019Quote: ... siRTN4 (L-010721-00-0005) and non-targeting Control siRNA (Cat#: D-001810-01-50) were from GE Life Sciences. 16% paraformaldehyde (Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... Device control utilized softWoRx 4.1.0 software (Applied Precision). Images were acquired using a 60x 1.4 NA Plan Apochromat oil immersion lens (Olympus ...
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bioRxiv - Biochemistry 2023Quote: ... and UNICORN Control Software version 5.11 (GE Healthcare) were used for all purifications ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Dharmacon DharmaFECT 1 transfection reagent (GE Healthcare, Manchester, UK) was used following the manufacturer’s siRNA transfection protocol with slight modification ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were performed using Dharmafect-1 (GE Healthcare) and 80nM of indicated siRNA ...
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bioRxiv - Cell Biology 2020Quote: ... or as a control Sepharose without IgG (GE Healthcare) for 3 hr at 4 °C rotating ...
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bioRxiv - Cell Biology 2023Quote: ... and RNAi negative control were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Physiology 2020Quote: ... After washing calmodulin and control Sepharose 4B beads (GE Healthcare) twice with lysis buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... siRNA and respective siRNA control were purchased from Dharmacon (GE Healthcare) and used at a final concentration of 25nM ...
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bioRxiv - Cell Biology 2020Quote: ... Control siRNA (negative) was obtained from GE Healthcare (D-001810-10).
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed 48h post-transfection with mammalian lysis buffer (GE Healthcare), then the luciferase substrate (Promega ...
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bioRxiv - Biophysics 2023Quote: ... transfection and enhancement using Hyclone Cell Boost 1 (GE Healthcare/Cytiva, SH30584.02) as described previously(32 ...
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bioRxiv - Cell Biology 2019Quote: ... using a DeltaVision climate control unit (37°C, 5% CO2) (Applied Precision). The kinetics of pre-stress followed by treatment was analysed from images captured at 2 min intervals for 100 min ...
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bioRxiv - Microbiology 2020Quote: ... We isolated C1 and control donor PBMCs by Ficoll-Plaque (GE Healthcare) density centrifugation.
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bioRxiv - Microbiology 2022Quote: ... control or mutant trophozoites were rinsed once in PBS (GE Life Sciences) and twice in serum free TYI-S-33 medium (SFM) ...
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bioRxiv - Biochemistry 2020Quote: ... The standard single-cycle kinetics method (Biacore T200 Control Software, GE Healthcare) was used ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting data were fitted with Biacore T200 Control Software (GE Healthcare) using a kinetic global fit 1:1 binding model ...
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bioRxiv - Microbiology 2019Quote: ... Arf1 and the control siGENOME nontargeting siRNA were purchased from GE Healthcare Dharmacon (Lafayette ...
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bioRxiv - Microbiology 2020Quote: ... 48 hours post-transfection cells were lysed with mammalian lysis buffer (GE Healthcare), then luciferin (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... siRNAs were transfected with DharmaFECT transfection reagent (#T-2001-02, GE Healthcare Dharmacon) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... Most transfections used tetracycline-screened HyClone Fetal Bovine Serum (FBS) (SH30070.03T; GE Healthcare). This enabled a faster cell recovery after transfection than with constructs using phleomycin selection that were originally used in cell lines and appeared somewhat less stable during subculture ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 4 μL of DharmaFECT1™ (GE Healthcare, UK), 144 μL of 1 μM siRNA (35 nM final concentration ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 0.1 μL of DharmaFECT1™ (GE Healthcare, UK), 3.6 μL of 1 μM siRNA (35 nM final concentration ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... targeting siRNA and non-targeting scramble control siRNA were purchased from GE Healthcare (mouse Dnmt1 siRNA-SMARTpool (L-056796-01) ...
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bioRxiv - Microbiology 2020Quote: ... 1D gel electrophoresis analysis was performed with Ettan IPGphor II control software (GE Healthcare). For 2D gel electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: ... Cell cultures were controlled using the UNICORN™ system control software (GE Healthcare, USA). Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... or the respective negative controls (non-targeting small interfering RNA, siCTR, GE Healthcare Dharmacon) using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific #13778-150 ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... DC control RNA was then purified using G-50 columns (GE healthcare, 25-5330-01). All samples were then prepared for sequencing using the randomer library prep workflow protocol described in Smola et al ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Cells were imaged 24 hours post-transfection on the DeltaVision LIVE high resolution deconvolution microscope (GE Healthcare) using the 60X/1.4 Plan-Apo NA oil objective (Olympus) ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry filter sets and a CoolSNAP HQ2 camera (Roper Scientific) under the control of SoftWorx (Applied Precision). 37°C and 5% CO2 were maintained in a TOKAI Hit stage incubator ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry filter sets and a CoolSNAP HQ2 camera (Roper Scientific) under the control of SoftWorx (Applied Precision). Live cells were imaged using an Olympus 40x UPlanFL N Oil NA1.3 objective in imaging medium (Leibovitz’s L-15 medium (Gibco™ 21083027 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Microbiology 2020Quote: ... Eight days post transfection the supernatant was loaded on the HiTrap protein G HP 1ml column (GE Healthcare) in binding buffer (20 mM sodium phosphate pH 7.0 ...
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bioRxiv - Cell Biology 2022Quote: ... antibody-antigen complexes were detected on X-ray films (FUJIFILM, Cat#47410-07523, Cat#47410-26615 or Cat#47410-07595) using an ECL system (GE Healthcare). Quantification was performed via densitometry using Fiji/ImageJ software (Schindelin et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... Secreted Fabs were purified from cell supernatants harvested four days post-transfection using Ni2+-NTA affinity chromatography (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 3 days post transfection and purified using GraviTrap TALON columns (GE Healthcare # GE29-0005-94). The columns were first equilibrated with purification buffer (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel images were created with ImageQuant LAS 4000 LAS4000 Image system using ImageQuant LAS 4000 Control Software (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of the proteins was performed by SDS-PAGE and size exclusion chromatography (Superdex200 10/300GL, GE Healthcare). The full amino acid sequences are presented in Supplementary Figure 2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL, GE Healthcare). The protein was digested with PNGase F (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... Sensorgrams were recorded using Biacore 3000 Control Software 4.1.2 and analyzed with BIAevaluation software 4.1.1 (GE Healthcare, Freiburg, Germany). The surface of flow cell 1 immobilized with the anti-histidine antibody was used to obtain blank sensorgrams for subtraction of the bulk refractive index background ...
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bioRxiv - Cancer Biology 2021Quote: ... sdRNA mimics and scrambled controls were ordered as custom miRIDIAN mimics from Dharmacon (GE Healthcare Dharmacon, Inc, Chicago, IL). Mimic and inhibitor sequences are detailed in Supplementary File 5 ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...