Labshake search
Citations for GE Life Sciences :
151 - 200 of 271 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... DNA was then purified using GFX PCR purification columns (GE Healthcare). DNA samples were diluted (1/200 for WCE ...
-
bioRxiv - Biochemistry 2023Quote: ... The PCR product was purified on a GFXTM column (GE Healthcare) and incubated with 1 mg of streptavidin-coated magnetic beads (Dynabeads M-270 ...
-
bioRxiv - Neuroscience 2022Quote: ... washed 3 x 5 min in 0.05% PBST at RT, and visualized using EZ-ECL Chemiluminescence (Biological Industries, 20500-120) by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences). Densitometric analysis was performed using ImageJ (NIH).
-
bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 5 min in 0.05% PBST at RT and protein bands were subsequently visualized by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences) using EZ-ECL Chemiluminescence detection kit for HRP (Biological Industries ...
-
bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
-
bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
-
bioRxiv - Bioengineering 2022Quote: ... cultured cells were fixed with 4% PFA in PBS for 20 min at room temperature (RT) and permeabilized and blocked with 0.1% Triton X-100 and 10% fetal bovine (FBS; GE Healthcare Life Science, Marlborough, MA) in PBS for 30 min at RT ...
-
bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
-
bioRxiv - Biophysics 2023Quote: ... Fluorescence images of oocyte chromosomes in utero were taken in the DAPI channel on a DeltaVision RT imaging system (Applied Precision, LLC; IX70 Olympus) equipped with a charge-coupled device camera (CoolSNAP HQ ...
-
bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
-
bioRxiv - Genomics 2020Quote: ... PCR products were purified using Strepdavidin Sepharose High Performance beads (GE healthcare). The beads bound to DNA were washed in 70% ethanol ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The PCR-products were purified using Illustra ExoProStar 1-Step (GE Healthcare) following the instructions from the manufacturer ...
-
bioRxiv - Genetics 2019Quote: ... PCR products were cleaned with GFX Illustra DNA Purification Kit (GE Healthcare); standardized aliquots were sent to Macrogen (http://www.macrogen.com ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR-product was inserted into the pGEX-5X-1 (GE Healthcare) according to the protocol above ...
-
bioRxiv - Biochemistry 2021Quote: ... PCR products were bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences) for immobilization ...
-
bioRxiv - Neuroscience 2022Quote: ... The PCR product was bound to Streptavidin Sepharose High Performance (GE Healthcare), purified ...
-
bioRxiv - Microbiology 2019Quote: ... PCR fragments were then labelled using Megaprime DNA Labeling Systems (GE Healthcare). Primers used are listed in Table 1.
-
bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
-
bioRxiv - Cell Biology 2024Quote: ... The PCR product was bound to Streptavidin Sepharose High Performance (GE Healthcare), purified ...
-
bioRxiv - Cell Biology 2020Quote: ... Differential interference contrast (DIC) and interference reflection microscopy (IRM) time-lapse imaging were carried out using the DeltaVision RT microscopy system (Applied Precision Inc., Issaquah, WA, USA), equipped with a ×60 oil immersion objective (1.40 NA ...
-
bioRxiv - Molecular Biology 2022Quote: ... The PCR product was purified on a MonoQ 10/100 column (GE Healthcare) with a 0.5–1 M NaCl gradient in 20 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare) is described in this protocol (dx.doi.org/10.17504/protocols.io.brzsm76e ...
-
bioRxiv - Microbiology 2020Quote: ... Purified PCR products were inserted into the pGEX-6P-1 vector (GE Healthcare) using BamHI and EcoRI restriction enzymes (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The PCR product was bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences), after which the immobilized PCR products were purified ...
-
bioRxiv - Genomics 2020Quote: ... PCR reactions were cleaned up using 1.5 volumes of SpeedBeads™ (GE Healthcare) in 2.5 M NaCl/20%PEG8000 and libraries size selected on a PAGE/TBE gels to 150–225 bp ...
-
bioRxiv - Plant Biology 2020Quote: ... The PCR products were purified either by ExoStar DNA purification reagent (GE Healthcare) or Wizard SV Gel and PCR Clean-Up System (Promega) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the PCR reaction was mixed with streptavidin beads (GE Healthcare #17-5113-01) and binding buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare), and sequenced in ABI3500XL Series automatic sequencer (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR product was purified with ExoStar (GE Healthcare Life Sciences, Pittsburgh, PA, USA), labeled with BigDye Terminator v.3.1 (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2023Quote: ... The biotinylated PCR products were immobilized on streptavidin-coated sepharose beads (GE Healthcare). DNA strands were separated using the PyroMark Q24 Vacuum Workstation ...
-
bioRxiv - Genetics 2023Quote: ... A radiolabeled PCR probe was prepared by random priming (GE Healthcare, Megaprime kit) using α-P32 dCTP and incubated with the membrane in commercial buffer (Ambion ...
-
bioRxiv - Pathology 2023Quote: ... homogenates were centrifuged at 500 x g for 5 min at RT and cell pellets were resuspended in a 36% Percoll solution (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) containing 100 UI/mL of heparin (HIBOR 5000 UI ...
-
bioRxiv - Cancer Biology 2020Quote: ... The PCR products were purified using streptavidin Sepharose HP beads (GE Healthcare, Buckinghamshire, UK), followed by hybridization with the sequencing primers (FOXL2-seqF ...
-
bioRxiv - Microbiology 2021Quote: ... gel-purified using illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). 30 ng of each upstream and downstream flanking region of the targeted gene were used as templates for overlap ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified according to the manufacture’s protocol (GE Healthcare, 28-9034-70) followed by Sanger sequencing ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR products were purified using Sera-Mag Select beads (GE Healthcare Life Sciences 29343052) and assessed for quality with Agilent High Sensitivity DNA kit (Agilent Technologies 5067-4626 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... purified with the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and then cloned into the pGEM-T Easy Vector (Promega ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... purified with the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare), and then quantified spectrophotometrically ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR products were cloned by Gateway® recombination into pGEX6P3 (GE Healthcare Life Sciences) and pEGFP-C1 (Clontech ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were purified using 0.2 μl ExoProStar 1-Step (GE Healthcare, Chicago, USA), 1.8 μl H2O and 8 μl PCR product ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were carried out using Illustra puReTaq Ready-to-go Beads (GE Healthcare). Fragments were cloned using pCR 2.1 TOPO TA cloning kit and sequenced ...
-
bioRxiv - Cancer Biology 2023Quote: ... PCR-amplified fragments were subcloned into pGEX-6P-1 (GE Healthcare, 28-9546-48). The following primers were used ...
-
bioRxiv - Molecular Biology 2020Quote: The third-stage PCR product was purified over a HiTrap Q HP column (GE Healthcare). Buffer A contained 10 mM NaCl and 10 mM NaHEPES pH 7.5 ...
-
bioRxiv - Physiology 2019Quote: ... PCR products were then bound to 2 μl Streptavidin Sepharose HP (GE Healthcare Life Sciences) in 40 μl Binding Buffer (Pyromark binding buffer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
-
bioRxiv - Biochemistry 2022Quote: ... and purified by Illustra GFX PCR DNA and a gel band purification kit (GE Healthcare). DNA sequencing was accomplished using 3130xl Genetic Analyzer (Biosystems ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products were cleaned with the Illustra Sephadex G-50 fine DNA grade column (GE Healthcare) according to the manufacturer’s recommendations and sequenced with a 3730xl Genetic Analyzer (Life Technologies ...
-
bioRxiv - Genetics 2021Quote: ... 10 μl of the PCR reaction was mixed with streptavidin beads (GE Healthcare #17-5113-01) by shaking for 5 minutes at room temperature and ...