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Citations for GE Life Sciences :
51 - 100 of 4503 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were diluted to 75 mM NaCl and loaded onto a MonoQ 5/50 GL (GE Healthcare) equilibrated in QO buffer (20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl of antiserum were coupled to 50 μl of recombinant protein A-sepharose (rPAS, GE Healthcare). Antibody beads were washed three times in PBS and treated with 5 μl fresh rPAS ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... protein extract was fractionated by anion exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare), washed with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography on a Superdex Peptide PC 3.2/30 column (GE Healthcare) using water/acetonitrile/TFA (77.4/22.5/0.1 ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
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bioRxiv - Biophysics 2021Quote: ... Peptides were desalted on a C18 MacroTrap column (Michrom Bioresources) and fractionated on a Superdex Peptide (GE Life Sciences) size-exclusion column ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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bioRxiv - Neuroscience 2019Quote: ... Cleaved protein was concentrated (50 kDa MWCO) and run on a Superdex 200 10/300 column (GE Healthcare) in SEC buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then purified by anion exchange chromatography on a MonoQ 5/50 GL column (GE Healthcare) using buffers AEX-A and AEX-B with a gradient of 7.5-50% B over 20 CV at a flow rate of 1 ml/min ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then incubated with 30 µl 50% protein A-Sepharose slurry (GE Healthcare, 17-0780-01) for 1 hr at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then incubated with washed and pre-blocked protein G Sepharose beads (50 µl, GE Healthcare) for 45 mins at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
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bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... treated with ECL prime blocking buffer (GE healthcare RPN418) for 1 hour and incubated with antibody at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... a Superdex Peptide 3.2/300 column (GE Healthcare) with 30% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... using a Superdex Peptide 3.2/300 (GE Healthcare) at a flow rate of 10 ţl min1 using 30% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... A Superdex Peptide 3.2/300 column (GE Healthcare) was used for SEC fractionation by isocratic elution with 30% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... a Superdex Peptide 3.2/300 column (GE Healthcare) with 30% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein at 50 µM was injected onto a Superdex 200 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted and fractionated by size exclusion chromatography (SEC) using a Superdex Peptide 3.2/300 column (GE Healthcare, Chicago, IL, USA) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in ECL advance blocking reagent (GE Healthcare) in TBS-Tween 0.1% and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... Both mutants carry a carboxy-terminal cmyc-peptide extension and a poly-His peptide extension for purification using nickel columns from GE Healthcare (Chicago, IL) and for anchoring in ELISAs as previously described and specified below (11–15).
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Molecular Biology 2022Quote: ... immobilized protein was eluted using wash buffer and further loaded onto an ion-exchange column (MonoQ 5/50 GL column, GE Healthcare) to achieve highly pure PBAF complex ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... total protein (50 μg) was subjected to SDS-PAGE followed by transfer to polyvinylidene difluoride membrane (GE Healthcare Life Sciences, Europe) using a semidry trans-blotter (BioRad ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... for 2h at 4 °C in Tris/NaCl buffer (50 mM Tris/300 mM NaCl and the tagged protein was purified over a HisTrap FF column (GE Healthcare). GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Neuroscience 2020Quote: ... which were then treated with ECL Prime blocking agent (GE Healthcare) and incubated with primary antibodies raised against the PS1 N-terminal (G1Nr5 ...