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Citations for GE Life Sciences :
1 - 50 of 4503 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10−18 M).[20] EDTA was added to formulations (4 eq with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Bioengineering 2022Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10-18 M).20,21 EDTA was added to formulations (4 molar equivalents with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Biochemistry 2019Quote: ... 50 μg of peptides were fractionated in a Shimadzu HPLC system using a Superdex Peptide 3.2/300 (GE Healthcare) at a flow rate of 50 μl/min using SEC buffer (30% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... After blocking in ECL blocking agent (GE Healthcare), membranes were probed with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 1mg protein was pre-cleared using 10µL of 50% protein G Beads (GE healthcare) by rotating for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... After blocking with 5% ECL Blocking Agent (GE Healthcare, RPN2125) at RT for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was divided equally and incubated with 50 μl of prewashed 50% Protein G slurry (GE Healthcare) for each condition ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified on a MonoQ 5/50 column (GE Healthcare) with a gradient of 100 to 1000 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in Blocking Buffer containing 5 % Blocking for ECL (GE healthcare) in Tris buffer for two hours ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μL of protein-G sepharose-4 fast flow (GE Life Sciences) (pre-washed and suspended in ...
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bioRxiv - Biophysics 2021Quote: ... followed by buffer exchange in (50 mM HEPES, 150mM NaCl, pH 7.5) using a Superdex peptide size-exclusion column (GE Healthcare) to give the fluorescence labeled degron and Ub-degron.
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl/minute ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 µL of supernatants were stored as input and the rest were incubated with the lysis buffer-prewashed G-sepharose beads (50 µL of 50% slurry per each reaction; Protein G-Sepharose 4 Fast Flow, GE Healthcare) for 3 h at 4 °C while rotating ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Microbiology 2020Quote: ... The protein was further purified by a Mono Q 5/50 (GE Healthcare; a linear gradient of 0–1 M NaCl in 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Molecular Biology 2022Quote: ... Eluted peptides were fractionated using a Superdex Peptide 3.2/300 column (GE Healthcare) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Physiology 2019Quote: ... Upon blocking the membrane with 2% (w/v) Amersham ECL Prime Blocking Reagent (GE Healthcare) for an hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with protein G-Sepharose resin (50% slurry; GE Healthcare, Waukesha, WI) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted peptides were fractionated on a Superdex Peptide 3.2/300 increase column (GE Healthcare) at a flow rate of 10 µL/min using 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: EDC-crosslinked FERRY peptides were separated on a Superdex Peptide PC3.2/30 column (GE Healthcare) at a flow rate of 50 µL min−1 ...
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bioRxiv - Cancer Biology 2022Quote: Size based fractionation of the peptides were done using Superdex Peptide 3.2/300 (GE Healthcare) column and HPLC (Agilent 1260 Infinity II) ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentrated protein sample was loaded onto a MonoQ 5/50 GL column (GE Healthcare) pre-equilibrated with Buffer A and exchanged into Buffer B [500 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... and 50 ul of 100mg/ml Protein A Sepharose Beads (17-0780-01, GE Healthcare) were added to the samples and incubated for 2 hours on rotating wheel at 4°C ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
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bioRxiv - Biophysics 2019Quote: ... Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2019Quote: ... For dot blotting 50-100ng of protein was blotted on nitrocellulose (Hydrobond-C Extra, GE Healthcare) and these filters were not fixed or boiled ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins (20-50 μM) were injected onto Superdex-200 Increase 10/300 column (GE Healthcare) for analysis at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 15 µL (30 µL 50% slurry) Protein A:Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Immunology 2019Quote: ... followed by the addition of 30 μl of a 50% slurry of protein G beads (GE Healthcare), and incubation overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The protein sample (50 μL) was loaded into a 10/300 Superdex® 200 GL (GE Healthcare) column connected to the MALS detection unit for SEC ...