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Citations for GE Life Sciences :
251 - 300 of 661 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the cell lysates in buffer D was incubated with Ni-NTA resin (GE Healthcare), washed using buffer D supplemented with 20 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Clarified lysates were loaded onto a Superose 6 10/300 GL column (GE Healthcare), attached to an analytical high-performance liquid chromatography system with in-line fluorescence detection (Shimadzu) ...
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bioRxiv - Cell Biology 2023Quote: ... clarified lysates were applied to a 5 ml HisTrap™ HP column (GE Healthcare) and washed with lysis buffer followed by a buffer containing 20 mM Tris-HCl pH 8.0 at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were added to a HiTrap Chelating HP 5 ml column (GE Healthcare) equilibrated with buffer A (20 mM Hepes pH 7 ...
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bioRxiv - Molecular Biology 2024Quote: Clarified lysate was passed over a 1-ml GSTrap Fast Flow Column (GE Healthcare) equilibrated with binding/wash buffer (20 mM phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared cell lysate was loaded on a 5 mL MBPTrap column (GE Healthcare). The captured proteins were washed with 5 CV wash buffer (20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... tuberculosis strains WT and ΔpknG were carried out for three biological replicates employing the Ettan DIGE System (GE Healthcare) following manufacturer’s recommendations (28 ...
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bioRxiv - Biophysics 2021Quote: ... This lysate was applied to a nickel affinity column (HisTrap FF 5mL ©Ge healthcare) pre-equilibrated with Ni-A buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Lysates were cleared by centrifugation and then incubated with Glutathione-Sepharose 4B resin (GE Healthcare) at 4 °C overnight and subjected to a series of salt washes (0.25M to 1M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... This pre-clarified lysate was subjected to cation-exchange chromatography.(Sepharose fast flow GE healthcare) for further purification ...
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bioRxiv - Systems Biology 2022Quote: ... The filtered lysate was loaded onto a 1 ml HisTrap FF (GE Healthcare, Freiburg, Germany) and unbound protein was removed with 20 column volumes of 15% buffer B (50 mM HEPES/KOH pH 7.8 ...
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bioRxiv - Biophysics 2022Quote: ... Protein was affinity purified from lysate using a HisTrap HP 5 mL column (GE Healthcare) in binding buffer (50 mM NaPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (200 μg) were incubated with 100 μL slurry of Streptavidin beads (GE Healthcare) for 1 h at RT with end-over-end mixing ...
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bioRxiv - Biochemistry 2020Quote: ... For purification of Usp21196-565 lysate was loaded ino a HisTrap FF cartridge (GE Healthcare) and eluted with 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... and the unclarified lysate was applied onto an IMAC HiTrap TALON crude column (GE Healthcare). The column was washed first with 50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were applied to MicroSpin S-400 HR spin columns (GE Healthcare, 27-5140-01). Total RNA was purified with Zymo RNA Clean & Concentrator-25 kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal protein amounts of lysate were incubated with streptavidin beads (17-5113-01, GE Healthcare) for 1 hour at 4°C before being washed and analysed by western blotting.
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bioRxiv - Microbiology 2020Quote: ... SepHMs-His6 was purified from lysates using an HisTrap column in ÄKTA pure (GE Healthcare) and eluted using an increasing concentration of imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was passed over a 5 ml Ni-NTA Fast Flow column (GE Healthcare) that had been equilibrated with Ni wash buffer (20 mM Tris pH8 ...
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bioRxiv - Microbiology 2019Quote: ... the lysate was loaded onto a Ni2+ column (HiPrep IMAC FF 16/10; GE Healthcare), followed by elution with a gradient of 10-250 mM imidazole in 2X PBS with 10 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: ... Soluble fractions of cell lysates were purified by a 5-ml HisTrap column (GE Healthcare) followed by a 5-ml HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... Clarified lysates were purified by affinity purification (GSTrap or HisTrap, GE HEALTHCARE, Little Chalfont, UK) and purity was assessed by SDS-PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was loaded on a 5 mL HisTrap HP column (GE Healthcare, Chicago, IL) pre-equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared cell lysate was loaded on a 5 mL HisTrap HP column (GE Healthcare). The column was washed with ClpX wash buffer (25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Bacterial cell lysates were loaded onto a HiTrap SP HP anion exchange column (GE Healthcare) and were then equilibrated with 50 mM HEPES/0.5 mM EDTA (pH 7.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was clarified by centrifugation and passed through a HisTrap HP column (GE Healthcare). The protein was then eluted with 10-250 mM imidazole gradient and the His-tag was cleaved off using His-tagged TEV protease expressed in-house ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were pre-cleared with 30 μl of protein-G-Sepharose (GE Healthcare Bio-Sciences). Pre-cleared supernatants were incubated with 5 μg of anti-SARS-CoV-2 nucleocapsid antibody mixture (GTX135357 and GTX632269 [6H3] ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that had been equilibrated with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Filtered lysate was loaded on a pre-equilibrated HisTrap nickel column (GE life sciences 17524701) using ÄKTA Start protein purification system ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysate was loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA) equilibrated with Buffer A (300 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 5 ml HiTrap Q HP column (GE Healthcare) equilibrated with TGE (7 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto HisTrap 5 mL (GE Healthcare, USA) using an imidazole gradient from 25 – 250 mM ...
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bioRxiv - Microbiology 2022Quote: ... The cleared cell lysate was loaded onto a 5mL HiTrap Chelating HP column (GE Healthcare) preloaded with NiSO4 as described by the manufacturer and equilibrated in buffer A ...
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bioRxiv - Neuroscience 2022Quote: Lysates were separated on SDS-PAGE gels and transferred to a nitrocellulose membrane (GE Healthcare). Membranes were blocked for 1 h at room temperature with either 5% milk in Tris-buffered saline plus Tween (TBST ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction of the lysate was applied to the Glutathione Sepharose beads (GE Healthcare), washed and eluted with PBS containing 25 mM of reduced glutathione ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was loaded onto a pre-equilibrated 5 ml HiTrap SP HP (GE Healthcare) column connected in series with 5 ml HiTrap Q HP (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant of cell lysate was incubated with Chelating Sepharose™ fastflow sepharose resin (GE Healthcare) charged with Ni2+ (according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate from each sample was loaded onto a HisTrap HP column (GE Healthcare) and eluted using 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that had been equilibrated with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that had been equilibrated with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was filtered (0.45 μm) and loaded onto a Ni-NTA column (GE healthcare) and eluted with 100% buffer B (buffer A + 500 mM Imidazole) ...