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Citations for GE Life Sciences :
501 - 550 of 661 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was filtered (0.8 μM) and applied to a 10 or 20 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare) (Maqbool et al ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was incubated for 45 minutes with 20 µl of Ni Sepharose 6 Fast Flow beads (GE Healthcare), washed and resuspended in 100 µl of buffer A (30 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (GE Healthcare Life Science). The membranes were blocked in phosphate-buffered saline with Tween 20 (PBST ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... into the periplasm and purified from the periplasmic lysate via anion exchange chromatography (Q-sepharose, GE Life Sciences Cat: 25236), using 20 mM Tris-HCl pH 8.0 with 20 mM NaCl (Buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to 0.2 μm nitrocellulose (GE Healthcare) for detection by western blotting ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and it was applied to a 5 ml GSTrap FF column (GE Healthcare, 17513101) to purify GST-proteins ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21(DE3) cells and purified from the cell lysate using Glutathione-Sepharose 4B resin (Cytiva, Tokyo, Japan (GE Healthcare)) ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was centrifuged at 20,000 x g and the supernatant was loaded onto a StrepTrap HP column (GE Healthcare). Protein was eluted in binding buffer with 2.5 mM D-desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was cleared from debris by centrifugation and the supernatant was incubated with 20 µl StrepTactin beads (GE Healthcare) for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell lysates were filtered with a 0.45uM syringe filter and loaded on to an equilibrated 5ml GSTrap HP column (GE Healthcare) using an Akta Purifier FPLC (GE Healthcare) ...
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bioRxiv - Genetics 2019Quote: ... Lysate was clarified by centrifugation at 25,000xg and soluble protein was purified using Co2+ affinity chromatography (TALON column, GE Healthcare) followed by anion-exchange chromatography (Q-FF ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate is then incubated overnight with 400ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) at 4°C with slow rotation ...
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bioRxiv - Biochemistry 2019Quote: ... 0.05% of Triton X-100 was added and the lysates were incubated with glutathione Sepharose 4B beads (GE Life Sciences) or Ni-NTA agarose beads (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Precipitated DNA was pelleted by centrifugation and soluble lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 7 column volumes of loading buffer before being eluted with a gradient of imidazole (20–500 mM ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant and the crude lysate were separately filtered using a 0.2 μm syringe Filter (Puradisc FP 30 Cellulose Acetate Syringe Filter, GE Healthcare) and directly loaded onto the column at 1 mL/min one after another ...
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bioRxiv - Molecular Biology 2021Quote: ... The clarified lysate was supplemented with 5mM βME then bound to a 1mL Ni-NTA column (HisTrap HP, GE Healthcare). The column was first washed with 20mL Lysis Buffer supplemented with 10mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cell Biology 2022Quote: ... NaCl was added to a final concentration of 0.25 M and the lysate was incubated with Glutathione sepharose beads (GE Healthcare) at 4°C for 1 hr with end-over-end rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 39 000 × g for 40 min and the supernatant loaded onto StrepTrap column (GE Healthcare) in lysis buffer containing 50 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... then both lysate and ESA samples were desalted and cleared of detergents using hydrophilic and hydrophobic SP3 beads (GE Healthcare cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated proteins were captured from lysates (500 µg per replicate) using 10 μg streptavidin sepharose (GE healthcare, Waukesha, Wisconsin, USA) for 8 h ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... lysates and immunoprecipitates were separated by SDS-PAGE with a 10% separation gel and transferred to nitrocellulose membranes (GE Healthcare). Following transfer ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...