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Citations for GE Life Sciences :
601 - 650 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... protein-containing fractions were concentrated and further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). 7H8 was purified in SEC-150 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were concentrated and further purified by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Heparin buffer A containing 150 mM KCl ...
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bioRxiv - Biochemistry 2020Quote: ... AcrB was then eluted with Buffer B containing 500 mM imidazole and directly injected onto a Superdex 75 10/600 GL size exclusion chromatography (SEC) column (GE Healthcare) equilibrated in buffer D (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing CI2 determined from SDS-PAGE analysis were concentrated and loaded onto a superdex75 10/300 GL column (GE Healthcare) equilibrated with 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The flow through containing SARS-CoV-2 Mpro was collected and further purified using size exclusion chromatography column (G-100, GE Healthcare,) equilibrated with 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through containing untagged Ire1 KR was further purified by applying it to a Superdex 200 16/60 gel filtration column (GE healthcare) and then concentrated to 20-40 μM in storage buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... fractions containing the intact CTD were pooled and loaded on a Superdex S200 16/60 size-exclusion chromatography column (GE Healthcare) using 25 mM Hepes ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
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bioRxiv - Microbiology 2020Quote: ... The bacteria lysate containing GST-Avr1d was incubated with 10 μl glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare) for 3 hours and purified by washing with 1×PBS for 5 times ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Microbiology 2019Quote: ... HSP40 fractions containing purified protein were pooled and further purified via anion exchange using a Mono Q anion exchange chromatography column (GE Healthcare) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Biophysics 2020Quote: ... Ni2+-NTA eluted fractions containing the polyprotein were concentrated to 10 mg/mL and further purified over a 10/300 Superdex 200 column (GE healthcare) in 20 mM phosphate buffer ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer A (devoid of ATP and PefaBloc but containing 200 mM instead of 30 mM imidazole) and further purified over a Superdex 200 10/300 GL column (GE Healthcare) in buffer B (25 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer F containing 200 mM imidazole and further purified over a 1 ml ResourceS cation exchange chromatography column (GE Healthcare) using a 20 ml gradient from 100 mM to 500 mM NaCl in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Immunology 2022Quote: ... the flow-through containing Fab fragments was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the enzyme were pooled and applied to a Sephadex™ G-25 (medium) (GE Healthcare, Piscataway, NJ, USA) column equilibrated and eluted in 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biophysics 2022Quote: ... Samples were eluted using buffer A containing 300 mM imidazole and were subsequently applied to a MonoQ 10/100GL column (GE Healthcare) and eluted using a linear NaCl gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... and the supernatant containing the soluble fraction was collected and filtered sterilized before purification in the ÄKTA Pure chromatograph (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... a pool containing Staphopain A was directly loaded on a HiPrep CM Fast Flow 16/10 (#28-9365-42, GE Healthcare) column equilibrated with NaAc5.5 and the column was washed until OD280nm reached a baseline ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Biochemistry 2021Quote: ... 0.125 pmol of FAM-labeled RNA product were separated by gel electrophoresis (20 % polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The elution containing NRG1β was concentrated with a 3K cutoff and applied on an S200 10/300 increase column (GE Healthcare). Protein from the major peak was stored in aliquots at −80 °C for subsequent receptor purifications ...
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bioRxiv - Biophysics 2022Quote: ... After dialysis the supernatant containing folded tetramers and dimers were subjected to Superose 6 prep grade XK 16/70 size exclusion column (GE Healthcare) purification using HFB buffer ...
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bioRxiv - Biophysics 2022Quote: ... The pooled fractions containing tdClamp-HaloTag were purified by size exclusion chromatography on a HighLoad Superdex 200 pg 16/60 column (GE Healthcare). Aliquots from combined fractions were frozen in liquid nitrogen and stored at -80°C until usage.
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bioRxiv - Zoology 2019Quote: ... The rehydrated IPG strips containing 100 μg of proteins were then subjected to isoelectric focusing as per the manufacturer’s standard protocol (GE Healthcare, USA). After IEF ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM PIPES pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM DTT and the flow-through (containing PFN1) was collected and then applied to a Superdex 75 (10/300) gel filtration column (GE Healthcare) equilibrated in 50 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... the protein of interest containing fractions were concentrated using an amicon ultrafiltration unit and applied to a suitable size-exclusion chromatography column (GE healthcare; for LPG1-1949 full-length and His6-MEK6/His6-MEK6 AcK210 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 32P-labeled DNA probes containing gag sequences were made by randomly primed DNA synthesis with Amersham Megaprime DNA labeling System (GE Healthcare). gag sequences were amplified by PCR from plasmids pGTy1-H3 (Genbank ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing plexin B2 were pooled and subjected to size exclusion chromatography with a Superdex 200 GL 10/30 column (GE Healthcare) equilibrated with Buffer C containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants containing the secreted RBDs were harvested at 60 hrs postinoculation and purified using a Ni-NTA column (GE Healthcare), followed by a Superdex 200 gel filtration column (GE Health care).
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bioRxiv - Immunology 2021Quote: ... The RBD containing flow through was further purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE healthcare). Proteins were flash frozen and stored at −80°C until use.
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bioRxiv - Immunology 2020Quote: ... The eluate containing the RBD was further purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) column pre-equilibrated in 2mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Developmental Biology 2019Quote: ... or 1 ng (for other mRNAs) of mRNAs in 1× modified Barths Solution (MBS) containing 4% Ficoll-400 (17-0300-05, GE Healthcare) using a microinjector (PLI-90 ...