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Citations for GE Life Sciences :
501 - 550 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed three times with TBS containing 0.1% Tween 20 and incubated with horseradish peroxidase-linked anti-rabbit IgG (NA934, GE Healthcare) at a dilution of 1:10,000 for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by SEC (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A with pH 7.5 as eluent ...
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bioRxiv - Molecular Biology 2020Quote: ... The homogenate was cleared by centrifugation at 30,000g and loaded onto a gravity-flow column containing Glutathione Sepharose 4B (GE Healthcare). The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... in a high-salt buffer containing 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl in an ÄktaPrime or ÄktaExplorer system (GE Healthcare). The column was calibrated with bovine erythrocyte carbonic anhydrase ...
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bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing MBP-AIM2 were pooled and purified by ion-exchange chromatography using 5 mL HiTrap-SP column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Bioengineering 2022Quote: ... PDMS replicas containing the microfluidic channel were punched with a 0.75 mm punch (Harris Uni-Core, GE Healthcare Life Sciences) at each end of the channel ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Developmental Biology 2020Quote: ... Minced mammary tissues were then digested in phenol-red free Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque) containing 2mM L-glutamine (GE healthcare), 1mg/ml collagenase (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and flow-through containing the cleaved SALM3-LRR was collected and gel filtered with Superdex 75 10/300 (GE Healthcare) in 60 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The fractions containing U1 snRNP were diluted to 100 mM NaCl and applied onto a heparin sepharose column (GE Healthcare). U1 snRNP was eluted with 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-containing fractions were then concentrated by microfiltration before loading on a Superdex 200 26/60 prepgrade column (GE Healthcare) column that had been equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The eluate containing the RBD was further purified by SEC using HiLoad 16/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated in 2mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The capped-protofibril containing solution was then applied to a Superdex200 26/60 PG SEC column (GE Healthcare, Piscataway, NJ) equilibrated with 20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2022Quote: ... Elution fractions containing Strep-RomY or StrepRomYN were loaded onto a a HiLoad 16/600 Superdex 200 pg (GE Healthcare) gel filtration column that was equilibrated with buffer A without imidazole for use in GTPase assays or buffer C for use in pull-down experiments ...
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bioRxiv - Microbiology 2022Quote: ... The TEV protease treated suspension was then transferred into the new columns containing 500 μl Calmodulin Sepharose 4B (GE Healthcare) and 6 ml of CBB buffer [150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to ice cold 15 ml tubes containing 1ml of IgG Sepharose 6 Fast Flow (GE Healthcare) and incubated on a rotating platform for 3 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant containing the 6xHis-tagged DMTases was stirred with 6 mL of NiCl2-charged chelating Sepharose beads (GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
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bioRxiv - Immunology 2022Quote: ... The purified protein buffer was exchanged to PBS containing 0.5 mM TCEP using a HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Aβ42 were lyophilised and further purified using a Superdex 75 HR 26/60 column (GE Healthcare, Chicago, IL), and eluates were analyzed using SDS−polyacrylamide gel electrophoresis for the presence of the desired protein product ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the protein of interest were applied to a Sephacryl 300 16/60 gel filtration column (GE Healthcare) and eluted with buffer D (10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... the supernatant containing eHEV was collected and filtered through a 0.45 um pore size filter (Whatman GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2023Quote: ... The nickel column fractions containing PLpro were pooled and loaded onto a HiLoad Superdex 200 size-exclusion column (GE Healthcare) on an ÄKTA pure 25 chromatography system (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Flow-through containing the untagged proteins was concentrated and loaded into a Superdex-200 10/300 GL column (GE Healthcare) pre-equilibrated with storage buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions containing Hsp27 were pooled and concentrated prior to loading onto a Sephacryl s300 size-exclusion column (GE Healthcare Lifesciences) previously equilibrated with 50 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... The elution fractions containing MalE-DmxA variants were pooled and loaded on a HiLoad 16/600 Superdex 200pg (GE Healthcare) SEC column ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-S8H (Tsai et al., 2018) as primary antibodies followed by detection with secondary antibodies (Agrisera; AS09 602, and GE Healthcare; A934-100UL, respectively). The chemiluminescence signal detection was performed using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies for chemiluminescence were anti-mouse (NXA931V, GE Healthcare), anti-rabbit (NA9340V ...
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: ... or anti-Flag (M5, Sigma; secondary antibody, NA931V, GE healthcare). Anti-Mam1 (ab155786) ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with HRP-conjugated secondary antibody (GE Healthcare) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... was used together with HRP-conjugated secondary antibodies (GE Healthcare), and SuperSignal West Pico and West Femto chemiluminescence substrates (Thermo Fisher ...
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bioRxiv - Developmental Biology 2019Quote: ... membranes were incubated with HRP-conjugated secondary antibody (GE Healthcare) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... and horseradish peroxidase-conjugated secondary antibodies (1:10000, GE Healthcare). Protein bands were visualised using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermofisher) ...
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bioRxiv - Plant Biology 2019Quote: ... Secondary antibodies were coupled to horseradish peroxidase (HRP, GE Healthcare) or alkaline phosphatase (AP ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated secondary antibodies were from GE Healthcare (rabbit, NA934) and R&D (sheep ...