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Citations for GE Life Sciences :
201 - 250 of 6278 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was harvested on day 5 and ACE2 protein was purified using HisTrap FF column (GE Life Sciences), followed by desalting ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The secreted antibodies were subsequently purified from the supernatant on HiTrap Protein G columns (GE Healthcare). Plasmid DNA encoding a gliadin-reactive IGHV4-4:IGKV4-1 antibody with R in position 55 (corresponding to the IGHV4-4*07 allele ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was concentrated and purified over HiTrap Protein G HP antibody purification column (GE Healthcare). To visualize 410C9 in vivo ...
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bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...