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Citations for GE Life Sciences :
1 - 50 of 6278 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 μg of GST-FG-repeat were incubated with 50 μL Glutathione Sepharose 4B beads (GE healthcare) at 4°C for 3 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... protein was loaded onto a 5 mL HisTrap column (GE Healthcare) and eluted with a linear imidazole gradient (20-350 mM ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified using 5 mL Ni-NTA column (GE Healthcare) and a HiLoad Superdex200 16/60 size exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified on a MonoQ 5/50 column (GE Healthcare) with a gradient of 100 to 1000 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Immunology 2021Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare) as described previously (Sok et al. ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare). Concentrations of purified mAbs was determined using a NanoDrop One (ThermoFisher).
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bioRxiv - Microbiology 2019Quote: ... protein was further purified using a 5 ml Q column (GE Healthcare). Protein was exchanged into 10 mM Tris-HCl pH 8.0 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by 5 ml Ni-NTA columns (GE healthcare) and eluted over an imidazole gradient (40-600 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were purified using a 5 mL HisTrap HP column (GE healthcare) followed by gel filtration (Superdex 30/60 GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Dialysed protein was passed through a 5 mL MBPTrap column (GE Healthcare). The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Biochemistry 2021Quote: ... Dialysed protein was passed through a 5 mL MBPTrap column (GE Healthcare). The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Immunology 2023Quote: ... A 5 ml protein G Sepharose 4 Fast flow column (GE Healthcare) was used to extract total human IgG ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was eluted by chromatography in 5-ml HisTrap (GE Healthcare) and digested with Smt3-specific protease Ulp1 (SUMO protease encoded in Rosetta pTB145) ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... antibody-coated protein G–Sepharose beads (GE Healthcare; 20 μl) were added to the mixture and incubated on a shaker overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Antibody–protein interactions were revealed using chemiluminesence (RPN2108, GE Healthcare), and relative protein expression was quantified using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The protein was further purified by a Mono Q 5/50 (GE Healthcare; a linear gradient of 0–1 M NaCl in 20 mM Tris-HCl (pH 8.0 ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was bound to a 5 ml HiTrap HP column (GE Healthcare), and a linear gradient of increasing NaCl concentration was used to elute the protein fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleaved protein was loaded onto a 5 ml Heparin column (GE Healthcare). The protein was eluted by a NaCl gradient from 0.25 to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed protein was loaded on a 5 mL HiTrap Heparin column (GE Healthcare) equilibrated with buffer A to remove the MBP ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was desalted using a 5 ml HiTrap Desalting column (GE Healthcare) and sterile-filtered PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subsequently purified over a 5 ml HiTrap Heparin column (GE Healthcare) and a 1 ml HiTrap Q column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein purification was performed using a 5 ml StrepTrap HP column (GE Healthcare) followed by SEC using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: Protein samples were loaded onto a HiTrap TALON column (5 mL, GE Healthcare) connected to an AKTA prime system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... the protein was passed through a 5 mL HisTrap HP column (GE Healthcare), and the cleaved protein was collected in the flow through ...
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...