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Citations for GE Life Sciences :
351 - 400 of 572 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... RPAPGST(GalNAc)APPAHG and RPAPGS(GalNAc)T(GalNAc)APPAHG with immobilized antibody 14A and 16A were determined by surface plasmon resonance on a Biacore T200 (GE Healthcare) instrument ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham) for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... and revealed manually on X-Ray films (Fuji; FOMA LP-T and FOMAFIX) or using a digital Amersham Imager 680 (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed several times in 0.05% TBS-T and proteins were detected using enhanced chemiluminescence (ECL) reagent (GE Healthcare; RPN2106). Antibodies used include ICAM-1 (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Systems Biology 2023Quote: ... After two additional washing steps with TBS-T and one with TBS the blots were developed by incubation with Amersham ECL Western Blotting Detection Reagents (GE Healthcare) for 2 min and detected on an ImageQuant (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... the rabbits were anesthetized via 2% isoflurane inhalation and imaged consecutively with clinical SPECT/CT scanner (Siemens symbia T16 SPECT/CT) and a 3.0 T clinical MRI scanner (Signa HDxt, GE Healthcare, USA) at 6 and 36 h post-injection ...
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bioRxiv - Neuroscience 2023Quote: ... the thoracoabdominal aorta of the rabbits was examined by MRI fast spin-echo (FSE) T2W imaging using a 3.0 T MR scanner (Signa HDxt, GE Healthcare, USA) and an 8-channel phased abdominal coil ...
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bioRxiv - Cell Biology 2020Quote: ... In a 48 well plate, 25-32.5μL siRNA (for sequences, see table 1) was mixed with 25uL 1x siRNA buffer (GE Healthcare) containing 0.5uL Dharmafect 1 transfection reagent ...
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bioRxiv - Genetics 2021Quote: ... Radioactive labelled oligonucleotide probes were purified with Illustra MicroSpin G-25 micro columns (GE Healthcare, Chalfont St Giles, UK).
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bioRxiv - Microbiology 2019Quote: ... the preparation was diluted in PBS and applied to a Sephadex G-25 column in PD-10 (GE Healthcare). Particle concentration and size distribution of the liposomes generated were evaluated using the NanoSight NS300 instrument (Malvern ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Genomics 2021Quote: ... DNA from the isolated pooled LL and HL fractions was desalted on PD MiniTrap G-25 columns (GE Healthcare) equilibrated in 10m Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... labelled probe was purified by native TBE-PAGE gel and illustra G-25 columns (GE Healthcare, Cat. No. 27532501). Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0 using a Centri Pure Zetadex-25 gel filtration column (Generon) and labelled with Cy3B maleimide (GE Healthcare) at room temperature for 2 hours using a 5 times molar excess of Cy3B ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified GST and GSTPib1ΔFYVE (100 pmol) were immobilised on glutathione Sepharose 4 Fast Flow (25 μl slurry, GE Healthcare). Beads were pre-treated with 1 mg/ml bovine serum albumin (Sigma) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Unincorporated γ-32P ATP was removed by running the product through Illustra MicroSpin™ G-25 Columns (GE Healthcare) and probe radioactivity was measured.
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bioRxiv - Biochemistry 2020Quote: ... as previously described 25 and eluted samples were desalted using PD10 columns according to the manufacturer’s instructions (GE Healthcare). Samples were applied to centrifuge filters (Vivaspin ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Plant Biology 2020Quote: ... Excess iron ammonium citrate and lithium sulfide was removed by desalting using a NAP™ 25 column (GE Healthcare). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... The conjugated Fab were then eluted from a PBS pre-equilibrated PD-mini G-25 desalting column (GE Healthcare) that removed unconjugated dye ...
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bioRxiv - Microbiology 2019Quote: ... ClpP containing fractions were pooled and further purified by size exclusion chromatography (ÄKTA pure 25 M1 FPLC; GE Healthcare) using a Superdex 75 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The ACP-TNB− was isolated and desalted using a Sephadex G-25 PD-10 desalting column (GE Healthcare, #17085101) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Microbiology 2020Quote: ... after which the supernatants were desalted on a Sephadex G-25 column (Illustra NAP-10 Columns, GE Healthcare, Pittsburgh) and concentrated by rotary evaporation ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess of DCP-bio1 was removed from the collected supernatant by filtration on Sephadex G-25 columns (GE HealthCare). The resulting samples were quantified using BCA and a minimum of 50µg of proteins were subjected to NeutrAvidin pull-down as described above ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction mixture was analyzed by analytical size exclusion chromatography on a Superdex 200 column (25 mL, GE Healthcare) as described above.
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Microbiology 2023Quote: ... the labelled oligonucleotides were separated from free [γ-32P] ATP using a custom Sephadex G-25 column (GE Healthcare). Labelled nucleic acids were incubated with nucleases (Rnase A ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were further washed three times for five minutes in PBS-T before being visualised using ECL kit (GE Healthcare Cat. #RPN2232) and ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: 3D structured illumination microscopy of fixed T cells cells was performed with a commercial Deltavision OMXv4.0 BLAZE microscope (GE Healthcare, Amersham, UK) using a 60x ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed again for 3 × 15 min in PBS-T and signals were detected with ECL Western Blotting Detection Reagent (GE Healthcare, #RPN2106) and acquired with Chemidoc MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Before revelation membranes were washed again 3 times in TBS-T for 5min and then incubated in ECL™ Western Blotting Detection Reagent (RPN2209, GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was diluted 4-fold with 25 mM Tris pH 8.0 and purified by anion-exchange chromatography using a Resource Q column (GE Healthcare), equilibrated in 25 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... elutes of KLP10A were subjected to buffer exchange to 1xMRB80 with 75 mM KCl and 0.1 mM ATP using a PD-25 column (GE Healthcare). AtMIDD1 was purified following (Oda and Fukuda ...
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare). A total of 20 injections with 2 μL each and a spacing of 180 seconds were titrated into the 200 μL protein sample (25 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: A volume of 25-30 µL serum was spotted onto a Whatman® Protein Saver™ 903 Card (GE Healthcare), air-dried at room temperature for approximately 2 hours and stored at 4 °C until further use ...
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...