Labshake search
Citations for GE Life Sciences :
501 - 550 of 572 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2020Quote: ... the aqueous phase was used for size-exclusion chromatography by using a disposable PD SpinTrap G-25 column (GE Healthcare Life Sciences). The purified tRNALeu was then ethanol precipitated ...
-
bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
-
bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
-
bioRxiv - Cell Biology 2021Quote: ... A 25 mL aliquot was layered over a 1.073 g·mL−1 Ficoll-Paque™ PLUS solution (GE Healthcare, Chicago, Illinois, United States) and centrifuged at 2000 x g for 25 min at room temperature (RT) ...
-
bioRxiv - Biochemistry 2020Quote: ... A flow-rate of 0.5 mL/min was applied for a minimum of 25 mL and 0.5 mL fractions collected (Äkta Frac-920, GE Healthcare Life Sciences). Size Exclusion Chromatography Multi-Angle Light Scattering was carried out in identical conditions to analytical SEC experiments (UFLC ...
-
bioRxiv - Bioengineering 2021Quote: ... at 25 mA for 1 h and transferred onto an Amersham™ Protran® 0.2 μm nitrocellulose blotting membrane (GE Healthcare, Canada) at 300 mA for 1.5 h ...
-
bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
-
bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
-
bioRxiv - Cell Biology 2019Quote: Experiments were performed at 25 °C with a Biacore™ X100 or T200 apparatus (Biacore™, GE healthcare Life Sciences, Uppsala, Sweden). The experiments were performed on biotin CAPture kit sensor chips (Biacore™) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
-
bioRxiv - Synthetic Biology 2020Quote: The antigenicity of BG505 SOSIP–T33_dn2A trimer and BG505 SOSIP–T33_dn2 nanoparticle was analyzed on a BIAcore 3000 instrument at 25 °C and with HBS-EP (GE healthcare Life sciences) as running buffer ...
-
bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
-
bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
-
bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
-
bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
-
bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
-
bioRxiv - Immunology 2023Quote: ... Each image captured 25% of the well and the percentage overlap was calculated using the Developer Toolbox software V1.9.2 (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Microbiology 2024Quote: ... the bacteria were resuspended at an OD600 = 0.5 U in three different nutritional culture conditions: (i) PBSG containing 25 mM HEPES (HyClone GE Healthcare Life Sciences), (ii ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gel was stained with ethidium bromide solution for 25 minutes followed by visualization on a Typhoon FLA 9500 fluorescence imager (GE Life Sciences) by selecting Cy3 scanning mode ...
-
bioRxiv - Cancer Biology 2019Quote: ... washed three times for 15min using 1x TBS-T and developed using ECL Prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA, USA). Each membrane was imaged using BioRad ChemiDoc and images analyzed using BioRad Image Lab software (BioRad) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 200ng Cas9 (PuroR) plasmid which express a puromycin resistant gene were co-transfected by 0.4 μL/well DharmaFECT Duo reagent (GE Healthcare Dharmacon, Cat #T-2010-03). After 48 hours of transfection ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
-
bioRxiv - Plant Biology 2020Quote: The enzymes’ buffer was changed to 10 mM MOPS (pH 7) using the PD Spintrap™ G-25 (GE Healthcare, 28-9180-04) protocol ...
-
Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... 1 h at room temperature) and cells were collected by gentle filtration onto polycarbonate membranes (25 mm diameter, 0.22 µm pore size, GE Healthcare Bio-Sciences, USA). Filters for counts of reporter deposition coupled with fluorescence in situ hybridization (CARD-FISH ...
-
bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We pooled fractions containing the purified protein, desalted the pooled fractions using PD-10 desalting columns packed with Sephadex G-25 resin (17085101, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
-
bioRxiv - Biophysics 2021Quote: ... gel filtration column with a flow rate of 1 ml/min using FPLC systems (AKTA pure 25 and AKTA FPLC, GE Healthcare Life Science). The 5 ml sample concentrated ...
-
bioRxiv - Neuroscience 2020Quote: ... The remaining elution buffer from the protein purification process was substituted by PBS using sephadex-based PD MidiTrap G-25 columns (GE Healthcare, Chicago, USA). Afterwards ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...
-
bioRxiv - Microbiology 2021Quote: ... 25 μL of PBS/serum were analyzed by size exclusion chromatography using MicroSpin G-50 columns (Sephadex G-50, GE Healthcare, Vienna, Austria) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged (12,500 rpm at 4°C for 25 mins) and purified by affinity chromatography on a 5mL HisTrap HP column (GE Healthcare Life Sciences). Proteins of interest were eluted using a linear gradient of 0 - 250 mM imidazole over 10 column volumes ...
-
bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 10 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
-
bioRxiv - Immunology 2021Quote: ... The elimination of imidazole was achieved by gel filtration on columns filled with 10 ml of Sephadex G-25 (GE Healthcare Life Sciences) previously equilibrated with ice-cold PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... were subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column connected to an AKTA PURE 25 FPLC system (GE Healthcare Life Sciences) maintained at 6° C ...
-
bioRxiv - Plant Biology 2022Quote: ... and the supernatant collected in a fresh 96-well deep well block and incubated with 25 µL of pre-equilibrated Ni-sepharose resin (GE Healthcare Life Sciences) in lysis buffer in a microplate shaker (Titramax 101 ...
-
bioRxiv - Neuroscience 2022Quote: ... The probe was end labeled with 35S-dATPα using terminal deoxytransferase and extracted using Illustra Microspin G-25 columns (GE Healthcare, North America). Each slide was then hybridized with 600,000 cpms of labeled probe in 125 μl of hybridization buffer and slides incubated overnight in a humid chamber warmed to 37° C ...
-
bioRxiv - Biochemistry 2023Quote: ... One to 2 L of seawater were filtered by pressure filtration through sequential 25 mm membrane filters housed in polypropylene filter holders (Whatman SwinLok, GE Healthcare, Pittsburgh, Pennsylvania) using a peristaltic pump and silicone tubing ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated for 30 minutes at room temperature and excess copper was removed by gel filtration using a PD MidiTrap G-25 desalting column (GE Healthcare; Uppsala, Sweden) equilibrated with acetate buffer (25 mM sodium-acetate ...
-
bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
-
bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: ... Whole genome DNA was amplified using 1 μl polymerase enzyme from the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Cat# 25-6600-32) and 9 μl of sample with the following conditions ...
-
bioRxiv - Biochemistry 2019Quote: ... Excess dye was removed by size-exclusion chromatography using PD-10 desalting columns containing Sephadex G-25 resin according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK). Concentration of the conjugate and degree of labeling (DOL ...
-
bioRxiv - Biochemistry 2020Quote: ... The clear upper phase of the lysate was desalted by size exclusion chromatography using a PD MiniTrap G-25 prepacked column (GE Healthcare, Chicago, IL, USA) with assay buffer as eluent ...
-
bioRxiv - Pathology 2019Quote: ... Equal amounts of protein extracts (25 µg) were separated by SDS-PAGE before electrophoretic transfer onto a nitrocellulose membrane (GE Healthcare, Velizy-Villacoublay, France). Western-blot analysis was carried out using anti-LC3-II (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: NFKBIA transcripts detected by smFISH in HeLa cells were obtained from a previous study (25) and were deconvolved with SoftWoRx using hardware specifications for the DeltaVision microscope (Applied Precision, GE Healthcare Life Science) used for the original image acquisition ...
-
bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
-
bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
-
bioRxiv - Microbiology 2023Quote: ... The gel filtration process was performed on an Äkta Pure 25 XK 16/70 column packed with 120 ml Superdex 200 (GE Healthcare, Chicago, IL, USA), and purified proteins were eluted with 1xPBS at 1 ml/min by collecting 2 ml per fraction ...