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Citations for GE Life Sciences :
401 - 450 of 5333 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were subsequently purified via affinity chromatography using HisTrap or MBPtrap columns connected to an ÄKTA Explorer liquid chromatography system (GE Healthcare) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal thioredxin-His6-tagged proteins were purified using Ni-NTA agarose affinity column followed by size-exclusion chromatography (Superdex 200 column, GE Healthcare) in the buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was purified with the His-tag affinity by chromatography with a HisTrap HP Ni2+sepharose column (GE Healthcare). The collected peak fraction was further purified through a HiTrap desalting column (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... and Neq2X7 were purified by affinity chromatography using a 1ml HisTrap™HP on an ÄKTA Pure chromatography system (GE Healthcare). After washing of the column ...
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bioRxiv - Immunology 2021Quote: ... and mAb was purified from spent medium by affinity chromatography on an ÄKTATM pure Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... TcsL was purified by nickel affinity chromatography followed by anion exchange chromatography using HisTrap FF and HiTrap Q columns (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Microbiology 2021Quote: ... and soluble supernatant was purified using HisTrap FF 5 mL immobilized metal ion affinity chromatography columns (GE Healthcare, New Jersey, USA) and eluted with 250 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... The recombinant proteins were purified from supernatants by affinity chromatography with a Ni-agarose column (HisTrapTM HP, GE Healthcare, Chicago, IL), dialyzed against PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... The poly-histidine-tagged G3BP-1-WT as well as the mutated S149A and S149E constructs were purified using immobilized metal affinity (IMAC, HisTrap FF, GE Healthcare) and the dimer was isolated using size exclusion chromatography on a Superdex 75 column (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Rac1 and Cdc42 were purified over GSTrap HP™ affinity columns with the FPLC AKTA system as per the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... were expressed in BL21(DE3) and then affinity-purified using glutathione Sepharose 4B beads (ABT) and HisTrap HP column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2019Quote: ... SapB-H6 was purified from conditioned media by nickel affinity and size exclusion chromatography with a Superdex 75 column (GE Healthcare) in cross-linking buffer ...
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bioRxiv - Molecular Biology 2019Quote: α-Syn was further purified with Ni-NTA affinity chromatography using a HisTrap HP 5 mL column (GE healthcare life sciences, USA) connected to an ÄKTA system (GE healthcare life sciences ...
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bioRxiv - Immunology 2019Quote: ... Strep(II)-tag proteins were purified using affinity column chromatography using a StrepTrap™ column prepacked with StrepTactin Sepharose by following the manufacturer’s protocol (GE Healthcare). The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6 ...
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bioRxiv - Neuroscience 2021Quote: ... and purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) followed by a Superdex 200 10/300GL column ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Immunology 2020Quote: ... Clarified Sf9 supernatants were filtered and MuSK1A was purified by immobilized metal affinity chromatography (IMAC) (Ni Sepharose excel, GE Life Sciences) followed by size exclusion chromatography (Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: Human his-tagged TRMT6/61A dimer was purified from Escherichia coli BL21 cells using 1 mL HisTrap HP affinity chromatography columns (GE Healthcare). Eluted fractions containing the recombinant proteins were pooled and incubated overnight with PreScission protease at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The protein was purified by submitting the supernatant to immobilized metal affinity chromatography (IMAC) using prepacked columns with Ni-Sepharose (HisTrapTM, GE Healthcare) in an ÄKTA Star protein purification system (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and AAV2-CMV-mKO) were produced using a helper-free triple transfection procedure and purified via affinity chromatography (GE Healthcare, USA).
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bioRxiv - Biophysics 2021Quote: ... U-13C-enriched proteins were purified through Ni-NTA affinity column and further by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using Superdex 75 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Fab and scFv constructs were eluted from affinity purification and then purified further via size exclusion chromatography using the Superdex 200 Increase 10/300 GL column (GE HealthCare) and 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble fraction was column-purified by immobilized metal ion affinity chromatography using Nickel sepharose high performance beads (17526802, GE Healthcare). The eluate was fractionated by gel filtration on a Superdex-200 Increase 10/300GL column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... His8-tag containing recombinant proteins were purified using Ni2+-NTA agarose affinity column followed by size exclusion chromatography (Superdex 200 26/60 column from GE healthcare) in a final buffer containing 100 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was harvested and the protein was purified by FLAG-affinity purification followed by MonoQ anion exchange chromatography (GE Healthcare). Finally ...
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bioRxiv - Immunology 2022Quote: ... The eluted product from kappa affinity column was pooled and further purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved His-SUMO tag and SenP1 was captured by passing through Ni-NTA affinity resin and flow-through was then purified by size exclusion (Superdex 75, GE Healthcare) chromatography in 50 mM HEPES pH 7 ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
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bioRxiv - Microbiology 2023Quote: ... PmScsC-EcDsbA was initially purified by immobilized metal affinity chromatography (IMAC) using a 5 mL HisTrap Fast Flow column (GE Healthcare) followed by cleavage of the N-terminal His6-tag with TEV protease and reverse IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The tag-less proteins were separated from the tags and His6-TEV protease by Ni2+ affinity chromatography and were further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with HSEC buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: nFhGST was purified by affinity chromatography from a Fasciola hepatica adult fluke extract using a 5/5 GSTrap HP column (GE Healthcare) following a pre-established protocol9 ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...