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Citations for GE Life Sciences :
651 - 700 of 5333 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... pH 7.4 (cytokines using Superdex 75 and receptors using Superdex200, prep grade media, GE Healthcare). In addition ...
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bioRxiv - Biophysics 2023Quote: ... The eluted receptor was desalted from imidazole using a size-exclusion PD10 column (GE Healthcare) equilibrated with the desalt buffer (25 mM HEPES pH=7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The Csm complex was captured on the HiTrap affinity column (GE Healthcare) by LdCsm2 copurification and eluted with buffer B [20 mM Tris-HCl (pH 8.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity purification was carried out with Glutathione Sepharose 4B resin (GE Healthcare). The His-GST tag was removed via TEV protease overnight at 4°C and cleaved protein was separated from the TEV and the His-GST tag with a Ni-NTA column ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was loaded on a nickel-NTA affinity column (GE Healthcare) and His6-AdhE was eluted with 300mM imidazole ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was loaded onto 1 mL Ni-affinity column (GE healthcare). After washing with the same buffer and the storage buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to a 5 mL Ni2+-NTA affinity column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatant was passed over a Ni-affinity column (GE Healthcare). His-GmPUB13 U-box and Avr1d were recovered by gradient elution with Ni-elution buffer (50 mM Tris [pH 8.0] ...
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bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded onto a HisTrap FF affinity column (GE Healthcare) and equilibrated with lysis buffer (see above) ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a Dextrin Sepharose affinity column (GE Healthcare) pre-equilibrated with 5 CV of binding buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... then passed over a nickel affinity column (HisTrap FF 5mL, GE Healthcare) and eluted with elution buffer (20 mM Tris-Cl pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was loaded onto Ni2+ affinity resin (Ni-NTA: GE healthcare) and washed with lysis buffer A containing 30 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: Affinity measurements were made using a Biacore T200 or 3000 (GE Healthcare). All experiments were performed at 37 °C using a flow rate of 10 µL min-1 in HBS-EP buffer (0.01 M HEPES buffer (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was load onto Ni2+ affinity resin (Ni-NTA: GE healthcare), wash and elution buffer are 30 mM and 300 mM imidazole in lysis buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was loaded onto Ni2+ affinity resin (Ni-NTA: GE healthcare) and washed with lysis buffer containing 30 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Affinity purification of GST-SseK3 was performed using glutathione sepharose (GE Healthcare), and purification of 6×His-SUMO-hUBE1 ...
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bioRxiv - Microbiology 2024Quote: ... affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare) and then concentrated to an optical density at 280 nm (OD280 ...
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bioRxiv - Neuroscience 2022Quote: ... and the soluble proteins were isolated by centrifugation (80,000g, 45 min, 4°C) and purified by cation exchange chromatography (HiTrap SP, GE Healthcare) with a gradient of 0-1M NaCl ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4°C and purified by a gel filtration HiLoad 26/600 Superdex 200 pg column (GE Healthcare, USA) using the same buffer.
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... was labeled with 99mTc (GE Healthcare, Seattle, WA) using the stannous tartrate method and the 99mTc – labeled albumin (T-Alb ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes from Qiagen were labeled with Cy3 monoreactive dye (GE Healthcare) and purified by ethanol precipitation and washing with 80% ethanol until the supernatant was colorless ...
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bioRxiv - Neuroscience 2022Quote: ... was labeled with 99mTc (GE Healthcare, Piscataway, NJ). A mixture of 240 mg/ml stannous tartrate and 1 mg/ml albumin was adjusted to pH 3.0 with HCl and one millicurie of 99mTc-NaOH4 was added to this mixture and incubated for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate made by sonication was centrifuged at 11,000 rpm for 30 min at 4◦C and the clear supernatant was purified through Ni-NTA column chromatography (GE Healthcare) following kit protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: Proteins were concentrated with appropriate centrifugal filtration devices (Amicon) and further purified by SEC at 4°C using an ÄKTAFPLC chromatography system (GE Healthcare). For crystallographic studies ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Biophysics 2019Quote: ... and protein was released from the resin by PreScission Protease (PPX) action at 4 °C overnight and further purified on a Superdex 200 column (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were digested at 4°C with specific proteases and further purified by ion-exchange chromatography using a HiTrap SP HP or monoQ (GE Healthcare) columns and a gradient running from 0% buffer A (40 mM TRIS ...
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bioRxiv - Immunology 2021Quote: ... Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... The sample was incubated at 4°C for 30 minutes and further purified using a HiLoad 26/600 Superdex 200 (GE healthcare).
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...