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Citations for GE Life Sciences :
651 - 700 of 754 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Time-lapse imaging of stable RPE1 cells expressing 53BP1-eGFP and Lifeact-RFP was performed in a 37°C climate chamber attached to a Deltavision microscope system and the SoftWorx 4.9 software (Applied Precision/GE Healthcare) using a 40x 1.3 NA oil-immersion objective ...
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bioRxiv - Biophysics 2020Quote: ... Cells were incubated for 3-5 days at 27 °C and transfection efficiency was monitored regularly by observing YFP expression level using a fluorescence microscope (DeltaVision, Applied Precision, LLC). At >50% transfection efficiency ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were clarified by centrifugation (18,000×g for 60 min at 4°C) and incubated with glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden), applied to a gravity column ...
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bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Cell Biology 2019Quote: ... The rest was incubated for 1 h at 4°C with 20 µl Ni Sepharose High Performance beads (GE Healthcare Life Sciences) to isolate the nanobodies ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Biophysics 2021Quote: ... The column eluate containing cyt c was further purified through a gel filtration Superdex 75 16/600 column (GE Healthcare, Chicago, IL) phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant was transferred to a 50 mL tube containing 250 µl of Glutathione Sepharose 4B beads (GE Healthcare, 17075601) washed twice beforehand in 50 mL of PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: Experiments were performed at 25 °C with a Biacore™ X100 or T200 apparatus (Biacore™, GE healthcare Life Sciences, Uppsala, Sweden). The experiments were performed on biotin CAPture kit sensor chips (Biacore™) ...
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bioRxiv - Cancer Biology 2020Quote: ... + RNA was first denatured by heating at 65°C for 5 minutes and then transferred to A cellulose nitrate membrane (Amersham, GE Healthcare, US) using A Bio-dot device (US Bio-RAD).The membranes were then uV-crosslinked ...
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bioRxiv - Synthetic Biology 2020Quote: The antigenicity of BG505 SOSIP–T33_dn2A trimer and BG505 SOSIP–T33_dn2 nanoparticle was analyzed on a BIAcore 3000 instrument at 25 °C and with HBS-EP (GE healthcare Life sciences) as running buffer ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
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bioRxiv - Microbiology 2020Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted in buffer C to reach 50 mM NaCl immediately before loading to a Q-sepharose HP column (5 mL, GE Healthcare, USA), previously equilibrated with 10 CV of Buffer C ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown in appropriate synthetic dropout media at 30°C to mid-log phase (OD600 of ∼0.5) and imaged using DeltaVision Elite system (GE Healthcare Life Sciences) equipped with an Olympus IX-71 inverted microscope ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
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bioRxiv - Cell Biology 2022Quote: ... for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science) and 3.2% acetic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM) and loaded onto HisTrap HP columns (GE Healthcare, Uppsala, Sweden). Then ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity-purified protein was further purified (>95%) using Superdex 75 prep-grade matrix in a 16/70 C column (GE Healthcare Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... 4°C for 30 min and added to a 50-mL Falcon tube holding 6mL of Glutathione-Sepharose® 4B resin (GE Healthcare) that was pre-equilibrated in the same buffer ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered with a 0.22 βM filter and loaded onto a tricorn 10/100 Capture Select C-tag XL™ resin (GE Healthcare, USA). The column was pre-equilibrated with 25 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Microbiology 2020Quote: ... This reaction was incubated at 37°C for 1 hour and cleaned up with ProbeQuant G50 spin columns (GE Healthcare, GE28-9034-08) as per manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2019Quote: ... Viral DNA was extracted from 500-μl subsamples using the Zymo Research Viral DNA kit following the manufacturer’s protocol and subsequently amplified isothermally at 30°C using Genomiphi Whole Genome Amplification Kit (GE Healthcare, Little Chalfont, UK). Samples were cleaned and concentrated using a Zymo Research DNA Clean and Concentrator kit ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were carried out at 37°C for 60 s and were terminated by rapid cooling (addition of ice-cold saline) and immediate vacuum filtration over a glass microfiber filters (Whatman GF/C, GE Healthcare, Illinois, USA). The radioactivity retained on the filters after thorough rinsing was quantified on Tri-Carb 2810TR Liquid Scintillation Analyzer ...
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bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...