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Citations for GE Life Sciences :
251 - 300 of 754 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... SPR was performed at 25°C using Biacore T200 system with CM5 chips (GE Healthcare). HBS-T (10 mM Hepes ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The binding study was carried out at 25°C in PBS-P buffer (GE Healthcare). The heparin molecule or fondaparinux at different concentrations were flowed over the immobilized recombinant proteins at a flow rate of 30 μl/min with a contact time of 60 s and a dissociation time of 100 s ...
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bioRxiv - Biochemistry 2020Quote: ITC experiments were carried out at 5°C with a MicroCal iTC200 instrument (GE Healthcare). Each of the ATAD2B bromodomain proteins and histone peptide samples were dialyzed into ITC buffer containing 20 mM NaH2PO4 pH 7.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: SPR measurements were carried out at 25°C using a Biacore X instrument (GE Healthcare) as described previously28 ...
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bioRxiv - Genetics 2019Quote: Widefield fluorescence imaging was performed at 20°C on a DeltaVision Elite system (Applied Precision) equipped with a 100 × /1.40 NA UPLSAPO oil immersion objective (Olympus) ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Neuroscience 2022Quote: Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS + 0.005% (v/v ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred onto PVDF membranes (Hybond-C Extra, GE Healthcare Life Sciences, Mississauga, ON, Canada). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... ITC experiments were performed at 25°C using an ITC-200 MicroCal microcalorimeter (GE Healthcare) following a standard procedure (Hands-Taylor et al ...
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bioRxiv - Bioengineering 2024Quote: Kinetic analyses were performed at 25°C using a BIAcore 3000 machine (GE Healthcare Technologies). A2p1 ...
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bioRxiv - Biophysics 2020Quote: Isothermal titration calorimetry measurements were performed at 25 °C in a MicroCal iTC200 System (GE Healthcare). The proteins were dialyzed simultaneously in the same experimental buffer (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Immunology 2019Quote: ... 4°C (SORVALL RC 6+) and supernatant was applied to a HisTrap affinity column (GE Healthcare) that was pre-equilibrated with binding buffer ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Cell Biology 2019Quote: Image stacks were acquired at 37°C using a DeltaVision imaging station (GE Healthcare / Applied Precision) fitted onto an Olympus IX-70 inverted microscope base ...
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bioRxiv - Cell Biology 2019Quote: Image stacks were acquired at 37°C using a DeltaVision imaging station (GE Healthcare / Applied Precision) fitted onto an Olympus IX-70 inverted microscope base ...
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bioRxiv - Neuroscience 2019Quote: ... For dot blotting 50-100ng of protein was blotted on nitrocellulose (Hydrobond-C Extra, GE Healthcare) and these filters were not fixed or boiled ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The chromatography was performed at 25 °C using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with Dialysis Buffer ...
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bioRxiv - Biophysics 2020Quote: ... and subjected to size exclusion chromatography at 4°C using a Superdex-200 column (GE Healthcare) and SEC buffer (50 mM Bis-Tris-Propane pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Equilibrium dissociation constants were obtained at 4°C using a MicroCal VP-ITC system (GE Healthcare). Peptide concentrations between 300-750 μM in the syringe were injected into 25–35 μM pocket domain in the cell ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Biophysics 2021Quote: ... incubated at 37 °C for 60 min and resolved using analytical Superdex S200 column (GE Healthcare) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... and then analyzed by size-exclusion chromatography at 4°C using a AKTAmicro system (GE Healthcare) mounted with a Superose 6 5/150 increase column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonanace (SPR) analysis was performed at 25°C using BIAcore T200 instrument (GE Healthcare). Wild type CcpA and V301A mutant proteins were immobilized on sensor chip CM5 via amine coupling ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: SPR experiments were carried out at 25 °C on a Biacore 8K SPR system (GE Healthcare). Series S SA sensor chips (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™, GE Healthcare) in plates containing ZYP agar medium (supplemented with 100 µg.mL-1 ampicillin and 34 µg.mL-1 chloramphenicol) ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were collected on Whatman GF/C filters with 1.2 μm pore size (GE Healthcare, US) under vacuum (750 mbar) ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... time-lapse movies of high resolution were recorded at 37 °C on a DeltaVision Elite (GE Healthcare) widefield microscope using a 60×/1.42 NA oil objective and a step size of 0.25-0.35 μm (Figure 1A-C ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Biochemistry 2019Quote: Yeast were imaged at 30 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision Ltd.) comprising a wide-field inverted epifluorescence microscope (IX70 ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were used for silverstaining (PageSilver kit, Fermentas) or transfer to Hybond-C nitrocellulose mebranes (GE Healthcare). After overnight blocking in PBS with 5% nonfat dry milk and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was filtered (1.2 μm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom), acidified to pH ≤ 1 for sterilization using 30% hydrochloric acid ...