Labshake search
Citations for GE Life Sciences :
401 - 450 of 1378 citations for Recombinant Human Interleukin 2 Receptor Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
-
bioRxiv - Biochemistry 2021Quote: ... an initial IMAC step was performed on a His-Trap column (all columns by GE Healthcare) using buffers A (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2019Quote: Proteins were purified according to their His6-tag using a His Excel column from GE Healthcare attached to a Äkta Start system ...
-
bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
-
bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
-
bioRxiv - Cell Biology 2022Quote: ... the elution fractions containing His-ZNF524 were further purified on a HiTrap Heparin HPTM (GE Healthcare). Therefore ...
-
bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
-
bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
-
bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
-
bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
-
bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
-
A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
-
bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
-
bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
-
bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
-
bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
-
bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
-
bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
-
bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
-
bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
-
bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
-
bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
-
bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
-
bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
-
bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
-
bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
-
bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 µl of equilibrated in lysis buffer His Mag Sepharose beads Ni (GE28-9799-17, GE Healthcare) was added to each sample and kept on a roller overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... the 6x His-MBP fusion was further purified on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
-
bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...