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Citations for GE Life Sciences :
651 - 700 of 1378 citations for Recombinant Human Interleukin 2 Receptor Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Immunoblots with anti-HA, anti-His or anti-SecB antibodies were visualized by Lumino image analyzer LAS-4000 mini (Cytiva, Tokyo, Japan (GE Healthcare)) using ECL Prime Western Blotting Detection Reagents (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Developmental Biology 2022Quote: ... The precipitants were dialyzed against a 400 times volume of PBS (-) plus Triton X-100 and then applied to a Nickel (Ni)-Sepharose Histidine-affinity column (His GraviTrap; GE Healthcare). Bound proteins were eluted with PBS (- ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was purified with the His-tag affinity by chromatography with a HisTrap HP Ni2+sepharose column (GE Healthcare). The collected peak fraction was further purified through a HiTrap desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... RAD51 without the his-SUMO tag was then recovered into the flow thru and directly loaded onto a HiTrap heparin column (GE Healthcare). The column was washed with W2 buffer and then a 0.1-1M NaCl gradient was applied ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates containing proteins with no His tag were purified by cation exchange chromatography (CIEX) using a 5 ml HiTrap Sepharose SP FF column (GE Healthcare) fitted on an ÄKTA fast protein liquid chromatography (FPLC ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Microbiology 2020Quote: ... Lysed cells were ultracentrifuged and the cleared supernatant loaded onto a Ni Sepharose 6 Fast Flow His Trap column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... with an anti-his-tag mAb (MBL) as the primary and anti-mouse IgG HRP linked whole Ab from sheep (GE Healthcare) as the secondary antibody ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through containing nsp12 was further purified by ion exchange chromatography using Hi TRAP Q HP and SP HP pre-packed columns (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Systems Biology 2023Quote: ... The lysates were clarified by centrifugation for 1h at 4ºC at 12000 rpm and loaded on a His GraviTrap™ column (GE Healthcare). After washing with 10 mL of washing buffer (40 mM sodium phosphate ...
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bioRxiv - Immunology 2022Quote: ... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
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bioRxiv - Plant Biology 2022Quote: ... radioactive signals from His-PIN1L were detected on the dried gel by the Typhoon imaging system (GE Healthcare TYPHOON FLA 9500).
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: Protein purification included two steps of immobilized metal-affinity chromatography separated by a thrombin-catalyzed His-tag cleavage and thrombin removal on a heparin column (GE Healthcare), hydrophobic interaction chromatography (HIC ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Bioengineering 2023Quote: ... coli lysates by immobilized metal affinity chromatography (IMAC) using a BioRad NGC FPLC equipped with a His-trap FF Ni2+ − NTA column (GE Healthcare). Columns were regenerated fresh with nickel prior to purification of each sample ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...