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Citations for GE Life Sciences :
151 - 200 of 2428 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2022Quote: ... and His-trap column (GE healthcare) previously equilibrated with Lysis Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates containing proteins with no His tag were purified by cation exchange chromatography (CIEX) using a 5 ml HiTrap Sepharose SP FF column (GE Healthcare) fitted on an ÄKTA fast protein liquid chromatography (FPLC ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
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bioRxiv - Immunology 2022Quote: ... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... using a His SpinTrap column (GE Healthcare). Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Microbiology 2023Quote: ... Anti-His antibody was from GE Healthcare; anti-PhoP antibody was elicited in rabbit (AlphaOmegaSciences) ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...