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Citations for GE Life Sciences :
51 - 100 of 2428 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Bioengineering 2022Quote: ... His-tagged Ri-boswitch-VLPs were purified using a Ni beads gravity column loaded with high-performance Ni beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial biomass was harvested by centrifugation at 5,400g and His-tagged nanobodies were purified using HisTrap™ column chromatography (GE Healthcare) followed by a buffer exchange step to PBS using a HiTrap™ desalting column (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Microbiology 2020Quote: ... and supernatants loaded onto 5 mL HP HIS-TRAP columns (GE Healthcare Life Sciences) equilibrated in buffer A (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4 and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of immobilised E141A H164A OmCI protein ...
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on an equilibrated 5 mL His-trap column (GE healthcare/Cytiva) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then passed through a 5 mL His-Trap FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...