Labshake search
Citations for GE Life Sciences :
251 - 300 of 6112 citations for Rat Heat Shock Protein Beta 1 HSPB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
-
bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
-
bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
-
bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
-
bioRxiv - Biophysics 2020Quote: ... The untagged proteins were further purified and concentrated using 1 mL HiTrapTM Heparin HP columns (GE Healthcare Life Sciences). The eluted proteins were analyzed on 10% SDS-PAGE gels and pure fractions (>95% purity ...
-
bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
-
Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... 100 μL of protein sample at 1 mg/mL was injected onto an S200 10/300 column (GE Healthcare) equilibrated in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
-
mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
-
bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
-
bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
-
bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
-
bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
-
bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-coupled secondary antibodies (anti-mouse and anti-rabbit, GE Healthcare; anti-rat, Jackson Immunoresearch) in combination with Clarity Western ECL Substrate (Biorad ...
-
bioRxiv - Immunology 2022Quote: ... and ECL anti-rat IgG Horseradish Peroxidase linked whole Ab from goat (GE Healthcare UK Limited) were used to detect IgG1 antibodies upon addition of TMB substrate (TMB substrate set ...
-
bioRxiv - Microbiology 2021Quote: ... The second antibodies used for ELISA were as follows: HRP-linked anti-whole rabbit IgG donkey serum (GE Healthcare, #NA934) for detection of the positive control ...
-
bioRxiv - Cell Biology 2020Quote: Protein-Protein interaction measurements were carried out on a BIACORE X100 (GE Healthcare Life Sciences). Sensorgrams were obtained as single cycle kinetics runs ...
-
bioRxiv - Molecular Biology 2019Quote: ... Approximately 1mg protein was pre-cleared using 10µL of 50% protein G Beads (GE healthcare) by rotating for 15 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mss116 protein was further purified by fast protein liquid chromatography (FPLC) Superdex 200 (GE Healthcare), the protein purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE ...
-
bioRxiv - Genomics 2021Quote: ... two sets of 20 µL of Protein A or Protein G Sepharose beads (GE Healthcare) were washed three times with nuclear lysis buffer for immunoprecipitation with antibody raised in guinea pig or in rabbit respectively ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sample protein masses were assessed with Amersham high molecular weight protein marker mix (GE Healthcare) with proteins porcine thyroglobulin (Rs=17.0nm) ...
-
bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
-
bioRxiv - Bioengineering 2023Quote: For all protein variants a protein A chromatography (MabSelect SuRe resin, GE Healthcare, Uppsala, Sweden) was used as capture step ...
-
bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
-
bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
-
bioRxiv - Cell Biology 2023Quote: ... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
-
bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
-
bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
-
bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
-
bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a hydrophobic HiTrap Butyl HP 1 ml column (GE healthcare life sciences), pre-equilibrated with 5 column volumes of buffer AButyl (500 mM ammonium sulfate ...
-
bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
-
bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
-
bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
-
bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
-
bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by incubation with protein A or protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 3 h with gentle rocking in the cold room ...
-
bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
-
bioRxiv - Biochemistry 2021Quote: ... Large scale protein purification was done using ÄKTA prime Fast Liquid Protein Chromatography System (GE Healthcare). DE droplets were analyzed on a BD LSR Fortessa SORP (Special Order Research Product ...