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Citations for GE Life Sciences :
451 - 500 of 6112 citations for Rat Heat Shock Protein Beta 1 HSPB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were bound to GST resin (GE Healthcare) and eluted in buffer A supplemented with 10 mM glutathione (pH-calibrated) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) by wet electroblotting ...
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bioRxiv - Immunology 2020Quote: ... PGT151-coupled Protein A Sepharose beads (GE Healthcare) were added to the supernatant and rotated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... proteins were transferred onto PVDF membranes (GE Healthcare). The human BCL2 protein was detected using the same mouse anti-human BCL2 antibody we use for flow cytometry followed by an HRP-linked anti-mouse Ig antibody (eBioscience ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare, Amersham Protran Supported 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were purified by Ni-NTA (GE Healthcare). MSLN specific VH domains were in the pComb3x vector and purified from Escherichia coli HB2151 bacterial culture at 30°C for 16 h with stimulation by 1 mM IPTG ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare cat.# 45000118) were mixed at 1:1 ratio before adding into the supernatant and rotating overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Monomeric protein was purified using Superdex200 (GE Healthcare) size-exclusion chromatography column on an AKTA Pure (Cytvia).
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bioRxiv - Immunology 2023Quote: ... proteins were transferred onto nitrocellulose membranes (GE Healthcare) at 10V constant voltage using OwlTM Semidry Electroblotting Systems (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified using glutathione resin (GE Healthcare) and eluted with lysis buffer supplemented with glutathione ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare) in a Tris-glycine transfer buffer containing 20% methanol ...
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bioRxiv - Cancer Biology 2024Quote: ... and Protein G Sepharose beads (GE Healthcare, 17061801) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: Affinities and kinetic parameters of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... Kinetic parameters and affinities of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was further purified with size exclusion chromatography to remove any protein aggregates applied to a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and a two-step fast protein liquid chromatography (FPLC) purification protocol using an ÄKTA Protein Purification System (GE Healthcare Life Sciences) at 8 °C was then followed to obtain the recombinant protein ...
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bioRxiv - Immunology 2022Quote: ... The culture was harvested after seven days and the supernatant purified into PBS by Protein G affinity using a HiTrap Protein G HP affinity column (GE Healthcare), eluted with 0.1M acetic acid (VWR international ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... a PCR fragment containing ubiquitin gene and a PCR fragment containing rat parkin residues 77-465 were introduced into the BamHI/XhoI sites of pGEX-6P1 (GE Healthcare). The serine (S77 ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...