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Citations for GE Life Sciences :
701 - 750 of 962 citations for Mouse IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The eluate was diluted with HEP A buffer to 500 mM (NH4)2SO4 and incubated with 20 mL IgG sepharose beads (GE Healthcare), equilibrated by washing with first 0.5 M AcOH pH 3.4 and second 1M Tris-HCl pH 7.5 (RT ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Neuroscience 2021Quote: ... Bands were detected using HRP-labelled anti-rabbit IgG and developed using an enhanced luminescence kit (GE Healthcare #GENA934, Watford, UK). Beta actin (Sigma #A5316 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... SOSIP proteins were isolated from cell supernatants using a 2G12 immunoaffinity column made by covalently coupling 2G12 IgG monomer to an NHS-activated Sepharose column (GE Healthcare). Protein was eluted with 3M MgCl2 followed by buffer exchange into TBS ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Immunology 2020Quote: ... Secreted Env trimers were isolated using PGT145 immunoaffinity chromatography by covalently coupling PGT145 IgG monomer to an activated-NHS Sepharose column (GE Healthcare) as previously described (81) ...
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody and ECL anti-rabbit IgG horseradish peroxidase-linked species-specific whole antibody (GE Healthcare). Membranes were treated with Westar ηC 2.0 chemiluminescent substrate (Cyanagen ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used for immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalogue number NA934) and HRP-conjugated donkey anti-mouse IgG (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... or HRP-conjugated donkey anti–mouse antibody (GE Healthcare, catalog number NXA931) or HRP-conjugated donkey anti-goat (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... ECL Secondary Mouse and Rabbit HRP antibodies used were from GE Healthcare.
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bioRxiv - Immunology 2021Quote: ... followed by peroxidase-conjugated anti-mouse antibodies (NA931, GE Healthcare, Chicago, IL). Bands were visualized using the ECL Select Western Blotting Detection Reagent (Amersham) ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with secondary anti-mouse Ig antibody (GE Healthcare) diluted with TBS-T for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were sheep anti-mouse HRP (GE healthcare; 1:5000) and donkey anti-rabbit (GE healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (mouse Aldh1a3, Clone ID 6515355, purchased from GE Healthcare) were inserted into pCAGIG vector (pCAGIG was obtained from Addgene (Plasmid # 11159).
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bioRxiv - Neuroscience 2022Quote: ... then with secondary antibody (donkey anti-mouse or antirabbit HRP, GE Healthcare) for 1 hr at room temperature before exposure using a ChemiDoc MP system (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated mouse and rabbit secondary antibodies were obtained from GE Healthcare. FemtoLUCENT™ PLUS HRP Kit (786-10 ...
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bioRxiv - Plant Biology 2019Quote: ... followed by HRP conjugated goat anti-mouse antibodies (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Pathology 2021Quote: ... cynomolgus or mouse LRG1 were determined by SPR (Biacore T200, GE Healthcare). All samples were buffer exchanged into running buffer HBS-EP+ ...
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bioRxiv - Cell Biology 2020Quote: ... Western blots were incubated in secondary donkey anti-mouse (GE Healthcare NA934V) or donkey anti-rabbit (GE Healthcare NXA931 or Jackson Immunoresearch 711-035-152 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were HRP-linked goat anti-mouse (1:10,000, GE Healthcare) and HRP-linked goat anti-rabbit (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Western blots were incubated in secondary donkey anti-mouse (GE Healthcare NA934V) or donkey anti-rabbit (GE Healthcare NXA931 or Jackson Immunoresearch 711-035-152 ...
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bioRxiv - Cell Biology 2024Quote: ... or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931; GE Healthcare) or HRP-conjugated donkey anti-goat antibody (catalog number sc-2020 ...
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bioRxiv - Microbiology 2024Quote: ... together with horseradish peroxidase conjugated sheep anti-mouse immunoglobulin G (GE Healthcare) as a secondary antibody (dilution 1:2000) ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoblots were washed and incubated for 1 h at RT with HRP-linked secondary antibody donkey anti-rabbit IgG (GE Healthcare, NA934). The HRP-signal was detected using SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...