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Citations for GE Life Sciences :
551 - 600 of 962 citations for Mouse IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Genetics 2020Quote: ... Proteins of interest were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (GE Healthcare). Peroxidase substrate was provided by ECL Western Blotting Substrate kit (Pierce) ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was then subjected to first round of affinity purification with IgG-SepharoseTM (GE healthcare, Germany) for 1 hour at 4°C on the rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA). Blots were developed using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... or anti-goat IgG antibodies and Amersham ECLTM Prime Western Blotting Detection Reagent (GE Healthcare, Buckinghamshire, UK). Band quantification was carried out using an Image Station 2000R (Kodak ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... The membrane was then incubated with an HRP-conjugated secondary antibody (ECL anti-rabbit IgG, GE Healthcare) and PstS bands were visualized with the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: Secondary antibodies for western blot analysis were ECL Plex goat anti-rabbit IgG-Cy5 (PA45012, GE Healthcare), ECL Plex goat anti-mouse IgG-Cy3 (PA43009 ...
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 1 × TBST with 5% non-fat milk: anti-rabbit IgG (1:10,000; NA934V; GE Healthcare) and anti-mouse IgG (1:10,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified supernatants were then incubated with 150 mL slurry of IgG beads (GE Healthcare, 17-0969-02) for 2h at 4 °C on a turning wheel ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected using either HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-linked sheep anti– mouse IgG (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-mouse (NA931, GE Healthcare, 1:10000). The guinea-pig anti-KCTD16 antibody was raised against a synthetic peptide derived from mouse KCTD16 (aa7-23 ...
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bioRxiv - Genomics 2020Quote: ... HRP-conjugated anti-mouse (GE Healthcare NA931V, 1:10000) and HRP-conjugated anti-rabbit (Santa Cruz sc-2004 1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-rabbit and anti-mouse secondary antibodies (GE Healthcare) were used in 2.5% milk-fat for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... followed by secondary HRP-conjugated anti-mouse (GE Healthcare) or anti-rabbit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were: α-Mouse-HRP (GE Healthcare), α-Rabbit-HRP (GE Healthcare) ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (GE Healthcare Cat# NA931, 1:5000 dilution), and then detected using an ECL SuperSignal West Femto y West Pico detection kit (Pierce).
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bioRxiv - Cell Biology 2022Quote: ... HPR-conjugated anti-rabbit or anti-mouse (GE Healthcare) was incubated with membranes ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid coding for mouse JNK3 cDNA (GE Healthcare) was used as a PCR template and the resultant product was subcloned into mammalian expression vector pRK5 downstream of a myc epitope tag ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cux2 cDNA were obtained from GE Healthcare (Clone ID 5727802 ...
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bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.
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bioRxiv - Microbiology 2021Quote: ... HRP-conjugated anti-mouse (NA931, GE Healthcare, IL USA), anti-rabbit (NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad) or anti-mouse antibodies (NXA931, GE Healthcare) conjugated to horse radish peroxidase were applied for 1 hr (1:10.000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... An a-mouse HRP-conjugated secondary antibody (GE Healthcare) was used at 1 ...
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bioRxiv - Microbiology 2023Quote: ... and anti-mouse-HRP antibody (GE Healthcare, Cat#NA931). Recombinant proteins included RBD peptide (RayBiotech ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-mouse M13-HRP (GE Healthcare Cat# 27942101) was added at 1:5000 dilution for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... the secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V) and developed using Clarity Western ECL Substrate (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... and sheep anti-mouse (GE Healthcare #NA931V, 1:1000). Protein bands were detected using Chemiluminescent Substrate (Thermo Fischer ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was clarified by centrifugation (65,000 rpm for 45 min) and incubated with IgG Sepharose (GE Healthcare) for 2 h at 12 °C ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Genetics 2019Quote: ... Primary antibodies were detected by HRP-conjugated anti-rabbit IgG (GE Healthcare NA934V, Lot 9636020; 1:10000 dilution) or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies are HRP-conjugated anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 111-035-144 or GE Healthcare, #NA934), HRP-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was purified by protein A-affinity chromatography with IgG Sepharose 6 Fast Flow resin (GE Healthcare), washed with PBS and eluted with 0.1 M glycine-HCl pH 3.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared supernatant was incubated with 250 μL of pre-equilibrated IgG beads (GE Healthcare, Little Chalfonte, UK) at 4°C for 1.5 h with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... The Secondly antibodies used were HRP-linked donkey anti-rabbit IgG antibody (NA934; GE Healthcare, Piscataway, NJ, USA) and HRP-linked donkey anti-mouse IgG antibody (NA931V ...
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bioRxiv - Immunology 2020Quote: ... Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure (GE Healthcare Life Sciences) column according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
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bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...