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Citations for GE Life Sciences :
651 - 700 of 6352 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Isothermal titration calorimetry (ITC) experiments were performed at 20°C following standard procedures using a Microcal VP-ITC calorimeter (GE Healthcare, Chicago, IL, US). The E ...
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bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified via a two-step chromatography process involving nickel-affinity chromatography via the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S200 10/300 GL column ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was performed at 37 °C for 16 h and terminated by desalting it to 50 mM ABC buffer with HiTrap Desalting column (GE Healthcare: Chicago, IL, USA). It was further purified using Ni-NTA resin and collected the flow-through to remove the side product ...
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bioRxiv - Microbiology 2020Quote: ... Filtrates were then filtered gently by vacuum (<400 mbar under-pressure) over pre-combusted GF/A filters (> 5 h at 460°C; 47 mm, GE Healthcare Whatman, Buckinghamshire, UK), and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque™ Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then dried at 80°C for 45 min and exposed overnight to a storage phosphor screen (GE Healthcare, BAS-IP SR 2025), which was subsequently imaged with Typhoon FLA700 gel scanner and quantified in Fiji/ImageJ.
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1 hour with a secondary antibody anti-Mouse (GE Healthcare, NA9310, 1:5000 dilution), washed three times with TBS-Tween 1 % solution and revealed with an ECL Western blotting detection kit (Advansta ...
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bioRxiv - Microbiology 2024Quote: ... for EF1-α - anti-mouse (1:10,000 in 1% milk/PBS-T, GE Healthcare, code NA931V). Following secondary incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Extracts were precleared for 1 h with protein A-Sepharose (GE Healthcare) supplemented with 1mg/ml BSA ...
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bioRxiv - Immunology 2020Quote: Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
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bioRxiv - Microbiology 2020Quote: Proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were applied to a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated with buffer I (40 mM Tris-HCl ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were isolated to magnetic beads (1 micron SeraMag beads; GE Healthcare) by the protein aggregation capture (PAC ...
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bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
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bioRxiv - Cell Biology 2020Quote: ... ECL Anti-Mouse IgG HRP linked secondary 1:5000 (GE Healthcare #NA931); ECL Anti-Rabbit IgG HRP linked secondary 1:5000 (GE Healthcare #NA934).
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bioRxiv - Neuroscience 2021Quote: ... or sheep anti-mouse IgG (GE Healthcare, cat #NA931, RRID:AB_772210, 1:10,000) in TBS-T containing 5% milk for 2 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were sheep anti-mouse HRP (GE healthcare; 1:5000) and donkey anti-rabbit (GE healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-linked sheep anti– mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-liked sheep anti-mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were HRP-linked goat anti-mouse (1:10,000, GE Healthcare) and HRP-linked goat anti-rabbit (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sheep anti-Mouse IgG-HRP (1:3,000, GE Healthcare, Cat# NXA931, RRID:AB_772209), donkey anti-Rabbit IgG-HRP (1:2,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant TSP2 protein was purified from CHO cells expressing mouse TSP2 via affinity chromatography with HiTRAP heparin HP (GE Healthcare) following protocols described by Oganesian et al ...
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bioRxiv - Microbiology 2020Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated for 1□h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was incubated for 1 h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Microbiology 2020Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare) yielding pure αMβ2 headpiece ...
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bioRxiv - Microbiology 2021Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been pre-equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded on a HiTrap Heparin HP column (1 mL, GE healthcare) equilibrated with buffer C ...
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bioRxiv - Immunology 2022Quote: ... through a 1 ml HiTrap Protein G HP column (GE Healthcare Life Sciences) previously equilibrated with Loading Buffer (0.02 M NaH2PO4 pH 7) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each protein was purified using a 1 mL HisTrap HP column (GE Healthcare), washing with 10 mM Hepes-NaOH ...