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Citations for GE Life Sciences :
601 - 650 of 6352 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or Anti-mouse IgG HRP-conjugated (GE Healthcare, 1:8000). Proteins visualization was performed using a SuperSignal West Pico Plus ECL substrate (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... mouse anti-β-tubulin 1:8000 (13-8000, GE Healthcare), mouse anti-Repo 1:20 (8D12 ...
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bioRxiv - Neuroscience 2020Quote: ... or sheep anti-mouse IgG-HRP (1:10,000, GE Healthcare) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... including ECL anti-mouse IgG (1:10000, GE HealthCare NA931V), anti-rabbit IgG-HRP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... HRP-coupled anti-mouse IgG1 (GE Healthcare, NA931; 1:20000) and HRP-coupled anti-rabbit IgG (Jackson ImmunoResearch,111-036-045 ...
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bioRxiv - Cell Biology 2022Quote: ... sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) or donkey anti-goat immunoglobulin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG-HRP (NA931V; 1/1000 dilution, GE Healthcare), anti-rabbit IgG-HRP (NA934V ...
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bioRxiv - Physiology 2021Quote: ... Results were normalized to the expression of β-actin (cat#: A3854, source: mouse, 1:80,000, Sigma-Aldrich; anti-mouse IgG (GE Healthcare; dilution: 1:25,000). Immunoreactive bands were visualized with an enhanced chemiluminescence detection system (ChemiDoc Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Microbiology 2019Quote: ... Viral DNA was extracted from 500-μl subsamples using the Zymo Research Viral DNA kit following the manufacturer’s protocol and subsequently amplified isothermally at 30°C using Genomiphi Whole Genome Amplification Kit (GE Healthcare, Little Chalfont, UK). Samples were cleaned and concentrated using a Zymo Research DNA Clean and Concentrator kit ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were carried out at 37°C for 60 s and were terminated by rapid cooling (addition of ice-cold saline) and immediate vacuum filtration over a glass microfiber filters (Whatman GF/C, GE Healthcare, Illinois, USA). The radioactivity retained on the filters after thorough rinsing was quantified on Tri-Carb 2810TR Liquid Scintillation Analyzer ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was centrifuged (12,500 rpm at 4°C for 25 mins) and purified by affinity chromatography on a 5mL HisTrap HP column (GE Healthcare Life Sciences). Proteins of interest were eluted using a linear gradient of 0 - 250 mM imidazole over 10 column volumes ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
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bioRxiv - Genetics 2019Quote: ... The reaction was incubated at 15 °C for 40 min and then purified using an illustra Sephadex G-50 column (GE Healthcare, Buckinghamshire, UK).
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were centrifuged for 20 min at 23000 × g at 4°C and filtered using Whatmann Mini-Uniprep filtering vials (GE Healthcare, Buckinghamshire, UK) with a regenerated cellulose membrane (0.2-μm pore size).
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Microbiology 2023Quote: ... The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare, Chicago, IL). Probe sequences are listed in Table S2.
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was exposed to a phosphoimager screen overnight at -20°C and the phosphoimager screen was scanned using the Typhoon FLA 9000 phosphoimager (GE Healthcare, Chicago, IL). Experiments were performed in triplicate to establish reproducibility ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Immunology 2024Quote: Affinity of bNAbs for Fcγ Receptors was assessed by SPR experiments performed on a Biacore T200 (GE Healthcare). SPR experiments were performed on a T200 apparatus at 25°C in PBS containing 0,05 % P20 surfactant (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
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bioRxiv - Microbiology 2021Quote: ... Cy3-labelled goat-anti-mouse mAb (PA43009, GE Healthcare, 1:3,000) was subsequently used as a secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1:2000 (Anti-mouse IgG: GE Healthcare Cat#NA931, RRID:AB_772210) and membranes were hybridized 90 minutes at room temperature with agitation on a Nutator (Clay Adams Brand) ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated sheep anti-mouse IgG (GE Healthcare NA931, 1:5000), HRP-conjugated goat anti-rabbit IgG (Cedarlane #111-035-144 ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-mouse or antirabbit HRP-conjugated antibodies (GE Healthcare, 1:5000).
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bioRxiv - Molecular Biology 2020Quote: ... ECL Sheep anti-mouse IgG-HRP (1:3000 GE Healthcare NA9310V) and ECL Donkey anti-rabbit IgG-HRP (1:3000 ...
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bioRxiv - Genetics 2021Quote: ... incubated with HRP-conjugated anti-mouse (GE Healthcare, #NXA931, 1:5000) and imaged in a Bio-Rad ChemiDoc imaging system.
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bioRxiv - Biophysics 2020Quote: ... anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare) for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Sheep-anti-mouse coupled to HRP (1:2000) (GENA931, GE Healthcare) was used as secondary antibody.
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bioRxiv - Cell Biology 2022Quote: ... or sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) and the enhanced chemiluminescence (ECL ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-mouse IgG (1:10,000; GE Healthcare) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Anti-mouse IgG-HRP antibodies (GE Healthcare, Cat#NA931; 1:5000) were used to detect the antibodies binding to RBD ...
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bioRxiv - Molecular Biology 2024Quote: ... HRP-conjugated sheep anti-mouse IgG (1:5000; GE Healthcare, NA931), HRP-conjugated goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Immunology 2019Quote: Collected mouse serum was pooled and IgG purified using protein G Ab SpinTraps (GE Healthcare Cat.# 28-4083-47) then concentrated and buffer-exchanged into PBS using Amicon Ultra 30K centrifugal filter units (Merck Millipore Cat.# UFC503024 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...