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Citations for GE Life Sciences :
1 - 50 of 4240 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... poly(A) mRNA was extracted using a QuickPrep Micro mRNA purification kit (GE Healthcare) from 20 sibling mid-stage embryos with extending limbs ...
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bioRxiv - Immunology 2020Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare), as described 74 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare) [21] ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Developmental Biology 2019Quote: ... or 1 ng (for other mRNAs) of mRNAs in 1× modified Barths Solution (MBS) containing 4% Ficoll-400 (17-0300-05, GE Healthcare) using a microinjector (PLI-90 ...
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bioRxiv - Cell Biology 2021Quote: ... mRNA was hybridized onto the Hybond-N+ membrane (GE Healthcare). After crosslinking spotted mRNA to membrane using Stratalinker 2400 UV Crosslinker ...
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bioRxiv - Microbiology 2022Quote: ... Cells that integrated the fluorescent marker were selected on TSA with 15 µg/mL gentamycin and confirmed for their ability to expression the mCherry fluorescent protein using a Typhoon FLA 9000 imaging system (GE Healthcare). The gentamicin resistance cassette was flipped by electrotransformation of the pFLP3a plasmid containing the FLP-recombinase into the newly produced fluorescent cells.
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bioRxiv - Genetics 2020Quote: ... The cap-enriched mRNA was prepared from 100 μg of total RNA with GST-eIF4EK119A protein and glutathione sepharose 4B (GE Healthcare), as described previously (Bilska et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... the mRNA was dissolved in HyClone HyPure Molecular Biology Grade RNase free water (GE Healthcare). DMEM cell culture media with high glucose ...
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bioRxiv - Immunology 2021Quote: ... and mRNA was transcribed into cDNA using the First-Strand cDNA Synthesis Kit (GE Healthcare). The Nb repertoire was isolated in 3 subsequent PCR reactions using the following primer combinations (1 ...
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and mRNA was reverse transcribed to cDNA using a First-Strand cDNA Synthesis Kit (GE Healthcare). The Nb repertoire was isolated in 3 nested PCR reactions using following primer combinations ...
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bioRxiv - Microbiology 2021Quote: ... Quantification of the full length mRNA was done by ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2020Quote: ... The mRNA was purified by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare) operated on Äkta FPLC system in buffer 30 mM Bis-Tris pH 6 ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of poly(A)+ mRNA was spotted on an Amersham Hybond-N+ membrane (GE Healthcare), followed by UV crosslinking ...
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bioRxiv - Biochemistry 2021Quote: ... the mRNAs (50∼100ng) were spotted directly onto a positively-charged nylon membrane (GE Healthcare, USA) and air-dried at room temperature for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... Three additional siRNAs targeting the 3’-UTR of KSRP mRNA (siKSRPe) were synthesized (Dharmacon, GE Healthcare) and pooled to a 20 µM stock solution ...
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bioRxiv - Immunology 2020Quote: ... and mRNA was reverse transcribed to cDNA using a First-Strand cDNA Synthesis Kit (GE Healthcare). The Nb repertoire was isolated in 3 subsequent PCR reactions using following primer combinations (1 ...
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bioRxiv - Microbiology 2020Quote: ... mRNA was purified from total RNA using Sera-mag oligo(dT) magnetic beads (GE Healthcare Life Sciences) and then fragmented in the presence of divalent cations (Mg2+ ...
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bioRxiv - Microbiology 2019Quote: ... mRNA was purified from total RNA using Sera-mag oligo(dT) magnetic beads (GE Healthcare Life Sciences) and then fragmented in the presence of divalent cations (Mg2+ ...
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bioRxiv - Cell Biology 2023Quote: ... followed by reverse transcription of mRNA into cDNA using a First-Strand cDNA Synthesis Kit (GE Healthcare). To isolate the Nb repertoire ...
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bioRxiv - Immunology 2021Quote: ... mRNA was mixed with RNA loading buffer and then carefully spotted onto a Hybond-N+ membrane (GE Healthcare), followed by UV crosslinking ...
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bioRxiv - Systems Biology 2023Quote: ... Ribosome protected mRNA fragments (RPFs) were enriched using MicroSpin S-400 columns (GE Healthcare, catalog # 27-5140-01). Columns were pre-washed with a 3mL polysome buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The mRNAs were further purified by filtration using MicroSpin™ S-200 HR columns (GE Healthcare, Chicago, IL, USA) to remove unreacted substrates (RNA reaction intermediates ...
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bioRxiv - Biochemistry 2020Quote: ASLPro containing a m1G37 modified anticodon stem-loop (18 nucleotides) and mRNA (19 nucleotides) were purchased from GE Healthcare Dharmacon and dissolved in 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Microbiology 2021Quote: ... quantification of the remaining mRNA at the different time points was done by ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... mRNA isoforms were visualised and quantified using an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Bio-Sciences AB). The RiboRuler High Range RNA Ladder (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Gel containing fluorescent protein samples were scanned with Typhoon FLA 9500 imager (GE Healthcare), using 473 nm blue LD laser/LBP (510LP ...
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bioRxiv - Cell Biology 2019Quote: ... The CiEB1-mCherry proteins expressed with histidine tag were purified using a Ni-NTA column (GE Healthcare) and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare) ...
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bioRxiv - Genomics 2020Quote: ... mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences). The mRNA was fragmented and used to prepare sequencing libraries according to the Illumina TruSeq Stranded mRNA sample preparation kit ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid coding for mouse JNK3 cDNA (GE Healthcare) was used as a PCR template and the resultant product was subcloned into mammalian expression vector pRK5 downstream of a myc epitope tag ...
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bioRxiv - Developmental Biology 2019Quote: ... Murine MRTFA coding sequence was acquired from GE Healthcare Dharmacon (MMM1013-202859509) ...
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescent CFUs were determined by imaging with a Typhoon FLA7000 fluorescent scanner (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... was used and the fluorescent signal acquired with a Typhoon fluorescent scanner (GE Healthcare). Band intensities were quantified using ImageQuant software (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... the M-Lipxtal protein was further purified by SEC using a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Cancer Biology 2020Quote: The full-length GATA3 and CRLF2 coding sequence were purchased from GE Healthcare and cloned into the cL20c-IRES-GFP lentiviral vector ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...