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Citations for GE Life Sciences :
301 - 350 of 4240 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with a linear 0.05 to 1.5 M NaCl gradient and further purified by gel filtration on a HiLoad Superdex 200 16/60 column (GE Healthcare) in 40 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The plates were subsequently developed in 1.5 M KH2PO4 (pH 3.64 ± 0.03) and autoradiographed using a Storm 860 Phosphorimager (GE Healthcare Life Sciences). pGpp signal was quantitated using ImageJ software.74 RelQ activity was expressed as the percentage of γ-32P-ATP substrate converted into product at each timepoint.73 Michaelis-Menten assays were performed by running the reactions for 5 minutes at the indicated concentration of GDP or GTP ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... 150 mM CsI or 1.5 M CsI in H2O or D2O prior to performing experiments using PD10 desalting columns (GE Healthcare). 10 μM freshly made ThT (abcam ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA (1 – 10 μg) was separated using formaldehyde (2.2 M) gel electrophoresis and transferred to a Hybond-NX Nylon membrane (GE Healthcare) via capillary transfer ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
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bioRxiv - Cell Biology 2022Quote: ... NaCl was added to a final concentration of 0.25 M and the lysate was incubated with Glutathione sepharose beads (GE Healthcare) at 4°C for 1 hr with end-over-end rotation ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... Chemiluminescence was detected using ECL substrate and imaged with FlourChem M (Biotechne) or Amersham Imager 600 (GE Healthcare Life Sciences) for membranes from 2D gel ...
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bioRxiv - Physiology 2022Quote: ... M-mode parasternal long axis view was applied using 11.5 MHz transducer (GE 10S-RS, GE Healthcare, Chicago, IL, USA), connected to an echocardiographic imaging unit (Vivid S5 ...
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bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the pads were sealed with a coverslip and fluorescent microscopy was performed with a DeltaVision Spectris Deconvolution Microscopes (Applied Precision, Issaquah, WA, USA). Cells were imaged for at least 8 stacks in the Z-axis from the middle focal plane with 0.15 μm increments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Immunology 2024Quote: 1μg of purified fosmid/BAC DNA purified using standard alkaline lysis protocol was labelled with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science) in a 50μl nick-translation reaction for 3h at 15°C.
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were exposed with ECL prime (GE Healthcare) at a ChemiDox XRS imaging system (Bio-Rad).Primary antibodies were used against H3K27me3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare) and detection was performed using anti-His primary antibody (Penta-His Antibody ...