Labshake search
Citations for GE Life Sciences :
401 - 450 of 731 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Neuroscience 2022Quote: ... a computed tomography (CT) and a T1-weighted anatomical MRI scan on a 1.5-T Signa Excite scanner (GE Healthcare) were acquired ...
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bioRxiv - Bioengineering 2023Quote: ... 13 fetuses at 23w to 37w GA scanned on a 1.5 T GE scanner (Signa HDxt, GE Healthcare, United States) were used for the following analysis ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biochemistry 2021Quote: ... the L proteins were further purified by size-exclusion chromatography (Superose 6, GE Healthcare) in buffer B ...
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bioRxiv - Neuroscience 2022Quote: ... The Biorad Bio-Dot apparatus was assembled according to the manufacturer’s protocol with Hybond N+ nylon membrane (GE Healthcare) on the top ...
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bioRxiv - Biochemistry 2022Quote: RNA samples separated by 11% WIDE Range Gel SDS-PAGE were transferred onto a Hybond-N+ membrane (GE Healthcare) and hybridized with biotinylated oligonucleotide(s ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Microbiology 2020Quote: ... VSG13 N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 50 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Neuroscience 2022Quote: ... The digested DNA was electrophoresed on a 0.8% agarose gel and transferred to a Hybond-N+ membrane (GE Healthcare). The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was separated on Agarose-Formaldehyde gels and blotted on a nylon membrane (Amersham Hybond-N+, GE Healthcare, RPN203B). RNA was cross-linked on membrane by UV light (2×240 mJoules ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was then blotted for 30 min at 20 V onto an Amersham Hybond-N membrane (GE Healthcare) and crosslinked to the membrane for 1 h at 50°C using a 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then applied to Ni2+-NTA (Nickel-Nitrilotriacetic acid) sepharose column (GE Healthcare, USA) pre-equilibrated with the lysis buffer at a flow rate of 0.2 ml/min ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) trifluoroacetic acid and applied to a Superdex 30 3.2/300 column (GE Healthcare). Fractions containing crosslinked peptides were evaporated to dryness ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Conjugation of inhibitors to beads was performed by carbodiimide coupling to ECH Sepharose 4B (CTx-0294885, VI16832 and PP58) or EAH Sepharose 4B (purvalanol B) (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... T cells were depleted by erythrocyte rosetting using sheep red blood cells and B cells were separated by Ficoll density gradient centrifugation as recommended by the manufacturer (GE Healthcare). The remaining erythrocytes were lysed in red blood cell lysis buffer (155 mM NH4Cl ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/RA of B-RAF (residues 150 -233) and RASSF5 (residues 199 - 367) were subcloned into pGEX-4T2 (GE Healthcare) to produce N-terminal GST fusion proteins ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Microbiology 2023Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Biophysics 2023Quote: ... we repeated the dilution in Buffer B to 18 mS/cm conductivity and loaded it onto HiTrap SP columns (GE Healthcare). For elution ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... All blots were washed 4X with PBS-T and then developed with a Amersham ECL Western Blotting Detection Kit (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times with TBS-T for 10 min each before incubation with Amersham ECL detection reagent (GE Healthcare) and chemiluminescent detection using the Li-COR Odyssey FC imaging system (LI-COR Biosciences) ...
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bioRxiv - Biophysics 2019Quote: ... USA) and an Optilab T-rEX refractometer (Wyatt Technology) in-line with a Superdex 75 10/300 GL (GE Life Sciences) size exclusion chromatography analytical column ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μg of each fusion protein were radioiodinated with 125I and Chloramine T hydrate and purified on a PD10 column (GE Healthcare), as described previously [38] ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of FN3Con-anti-VEGFR2 was measured using surface plasmon resonance with a 30 µl/min flow rate at 25 °C (BIAcore T-100, GE Healthcare). VEGFR2 domains (SiboBiological ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:10,000 in TBS-T with 3% BSA and finally washed 3 x in TBS-T for 10 min before adding ECL substrate for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...