Labshake search
Citations for GE Life Sciences :
651 - 700 of 731 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... All recombinant proteins were purified using Ni2+-nitrilotriacetic acid agarose (Ni-NTA) column followed by size-exclusion chromatography (Superdex 200 column from GE Healthcare) in a final buffer containing 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2019Quote: STAT3 siRNA knockdown utilized the ON-TARGETplus Human STAT3 siRNA kit from GEHealthcare (L-003544-00-0005, GE Healthcare). This SMARTpool siRNA contains four pooled siRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... approximately 30 μg of total RNA was denatured and separated on a 1% denaturing formaldehyde agarose gel and transferred to a Hybond-N+ nylon membranes (GE Healthcare Life Sciences, London, UK). PCR products were labeled with DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Neuroscience 2019Quote: ... L cells (ATCC® CRL-2648) were cultured in high glucose Dulbecco’s modified Eagle medium (GE Healthcare, Piscataway, NJ, USA) plus 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... siRTN4 (L-010721-00-0005) and non-targeting Control siRNA (Cat#: D-001810-01-50) were from GE Life Sciences. 16% paraformaldehyde (Cat# ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Immunology 2019Quote: ... RPMI 1640 with L-glutamine and 25 mM HEPES (cat. # SH30255.01) and ECL reagent (cat. # RPN2106) were from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Developmental Biology 2020Quote: ... Minced mammary tissues were then digested in phenol-red free Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque) containing 2mM L-glutamine (GE healthcare), 1mg/ml collagenase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2023Quote: ... # 240-B) in conditions of molecular crowding (37.5 mg/ml Ficoll 70 and 25 mg/ml Ficoll 400, GE Healthcare, Cat. # 17-0310-10 and # 17-0300-10) and vitamin C supplementation (0.2 mM ...
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bioRxiv - Neuroscience 2019Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution fractions were loaded onto a SuperoseTM6 10/300 GL column previously equilibrated with buffer L using an ÄKTA Pure system (GE Healthcare). The purified His-SctV9-PR Peptidisc complex was subsequently analyzed by negative-stain electron microscopy.
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bioRxiv - Biophysics 2019Quote: ... 60 - 120 µL of extruded sample were buffer-exchanged through 1.5- to 3.0-mL Sephadex G-50 Fine resin (GE Healthcare, Chicago, IL) columns equilibrated with buffer F (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... of the purified hPIV3 L-P complex was determined by analytical size-exclusion chromatography using a Superdex 200 Increase 5/150 GL column (GE healthcare) with buffer B ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...