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Citations for GE Life Sciences :
551 - 600 of 1227 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL, GE Healthcare). The protein was digested with PNGase F (New England BioLabs ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were filtered (0.45 μm) to remove residual cellular debris and loaded onto a His-Trap Column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The concentrated sample was further purified over a Superdex 200 Hi-load 16/60 gel filtration column (GE Healthcare) pre-equilibrated with 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: The interaction between PttP1 and PttP2 was studied by a pulldown assay using His SpinTrap™ columns (GE Healthcare) with a bed volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: ... tag cleavage and dialysis followed by gel filtration on a Superdex 75 Hi Load column 16/60 (GE Healthcare) pre-equilibrated with 20mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... and loaded to a Hi-Load 16/600 Superdex-200 pg (preparation grade) size-exclusion chromatography column (GE Healthcare) equilibrated with the gel filtration buffer (100 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... His-2xFYVE-containing fractions were pooled and subjected to size exclusion chromatography using a Superdex 75 column (GE HealthCare). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and proteins were electrotransferred to nitrocellulose membranes (Amersham Hybond-ECL, GE Healthcare, Chicago, IL, USA). The membranes were incubated overnight at 4°C with primary antibody (Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... Excess dye was removed using a PD-10 desalting column (GE Healthcare, Chicago, IL, USA). Titrations were performed in PBS using a constant 1 μM concentration of fluorescein-coupled peptide with increasing concentration of R7R8 fragment (either wild type or vvv mutant) ...
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bioRxiv - Immunology 2020Quote: ... media containing secreted SC was applied to Ni Sepharose Excel resin (GE Healthcare, Chicago, IL), washed with buffer B (50 mM NaPO4 ...
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bioRxiv - Plant Biology 2022Quote: ... The bait protein GST-WL1 was incubated with GST beads (GE Healthcare, Chicago, IL, USA) in non-EDTA buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and pellets were filtered through a PVDF 0.2 µm syringe filter (GE Healthcare, Chicago, IL) and centrifuged further at 186,000 x g for two hours at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... The generated ADC was purified with PD SpinTrap™ G25 column (GE Healthcare, Chicago, IL) or high-performance liquid chromatography (Waters ...
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (GE Healthcare Life Sciences, Chicago, IL) density gradient centrifugation at 400 x g for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by HiTrap HP cation exchange chromatography (GE Healthcare, Chicago, IL) and Superdex 75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... Echocardiograms were performed with the GE Healthcare Echocardiography Vivid 7 system (GE Healthcare, Chicago, IL) equipped with an i13L probe ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... pH 7.5 and filtered through a 0.22µm polyether-sulfone membrane (GE Healthcare, Chicago, IL, USA) to remove any aggregates ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were detected using ECL™ Western Blotting Detection Reagents (RPN2209, GE Healthcare, IL, USA). As a control ...
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bioRxiv - Cancer Biology 2019Quote: ... IgGs in supernatants were purified through a HiTrap protein A column (GE Healthcare, Chicago, IL). Antibodies were eluted with 0.1M glycine pH 2.7 ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to the nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA), blocked with 3% BSA for 2 h ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, IL). Membranes were rinsed in PBS + 0.05% Tween-20 before visualization.
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
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bioRxiv - Bioengineering 2022Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated with Ficoll-Paque Plus (GE Healthcare, Chicago, IL) from human blood ...
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bioRxiv - Cell Biology 2022Quote: ... the blots were developed using an enhanced chemiluminescence kit (ECL Prime, GE Healthcare, IL, USA).
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bioRxiv - Plant Biology 2021Quote: ... The proteins were transferred from SDS-PAGE gel to PVDF membrane (GE healthcare, Chicago, IL). Membrane blocking and antibody incubations were performed using iBind western device (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was treated with ECL Select western blotting detection reagent (GE healthcare, Chicago, IL) and signal was detected with Licor Odyssey imaging system (LI-COR Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands were visualized with ECL Prime Western Blotting Detection Reagent (GE Healthcare, IL, USA) on a Light Capture II instrument (ATTO ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins recognised by the antibodies were visualised using ECL prime (GE Healthcare, Chicago, IL, USA) and digital images were acquired with a LAS4000 mini imager (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Microbiology 2022Quote: ... Bands were visualized with ECL Prime Western blot detection reagents (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2022Quote: ... cell culture supernatant was harvested and purified with a Protein A (GE Healthcare, Chicago, IL) column ...
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bioRxiv - Biochemistry 2022Quote: ... followed by GST column purification using the ÄKTA start chromatography system (GE Healthcare, Chicago, IL). Cell lysate supernatant was added to the column containing nickel resin and it was recirculated across the column by a peristaltic pump overnight at 0.5 ml/min ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence-labeled protein was visualized with a Typhoon trio fluorescence scanner (GE Healthcare, Chicago, IL) and relative protein quantities were determined using FIJI (Schindelin et al. ...
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bioRxiv - Biophysics 2022Quote: ... the protein sample was loaded on an S75 gel-filtration column (GE Healthcare, Chicago, IL). Pure fractions were collected and dialyzed against 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... and separated on a Superose 6 Increase column (GE Healthcare Life Sciences, Chicago, IL, USA) on an NGC Chromatography system (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2019Quote: ... and filter sterilizing through 0.2 μm syringe filters (Whatman GE Healthcare Life Sciences, Chicago, IL) into growth medium ...
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bioRxiv - Plant Biology 2019Quote: ... The supernatant was filtered using a 0.45-μm polypropylene filter plate (GE Healthcare, Chicago, IL). Samples were separated by reversed-phase chromatography on an Ultimate 3000 HPLC (Dionex ...