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Citations for GE Life Sciences :
451 - 500 of 1227 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Spin columns consisted of Sephadex G-50 Fine resin (GE Healthcare, Chicago, IL) equilibrated with pH-adjusted buffer F2 and subjected to a pre-spin at 1100 g for 25-30 s ...
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bioRxiv - Cell Biology 2021Quote: ... using a GE ImageQuant LAS 400 (Cat# 28955810, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2020Quote: ... Samples were separated on an ÄKTA purifier UPC10 (GE Healthcare, Chicago, IL, USA) consisting of a INV907 column switcher ...
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bioRxiv - Immunology 2021Quote: ... female = 5) were isolated by ficoll-hypaque density gradient (GE Healthcare, IL, USA) from the buffy coats purchased from the New York Blood Center (New York ...
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bioRxiv - Molecular Biology 2020Quote: ... Media was filtered through 0.2 μm PVDF filtered syringes (GE Healthcare, Chicago, IL) or PVDF vacuum filters (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... and supplemented to determine culture optima using [200U/mL] IL-2 (GE Healthcare), [155U/mL] IL-7 (Miltenyi Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (GE Healthcare, Chicago, IL) density gradient centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... followed by transfer by electroblotting to nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). TSPP1 was detected using a rabbit crude antiserum (dilution 1:500 ...
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bioRxiv - Biophysics 2022Quote: ... and then loaded onto a Superose 6 Increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... RNA was chromatographically purified using Capto Core 700 resin (GE Healthcare, Chicago, IL) followed by diafiltration and concentration through tangential flow filtration using a 750 kDa molecular weight cut-off (MWCO ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of alkylation solution [100 mM iodoacetamide (GE Healthcare, Chicago, IL, USA)] was added and incubated protected from light for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Detection was done with commercial ECL peroxidase assay (GE Healthcare, Chicago IL USA) with a Chemidoc (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... connected to an echocardiographic imaging unit (Vivid S5, GE Healthcare, Chicago, IL, USA). All parameters were analysed by an investigator in a randomised and blinded manner ...
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bioRxiv - Synthetic Biology 2023Quote: ... Detection was done with commercial ECL peroxidase assay (GE Healthcare, Chicago IL USA) with a Chemidoc (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: Images were obtained on a microscope (Olympus IL-X71 Delta Vision; Applied Precision) equipped with 100× NA 1.40 objective ...
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bioRxiv - Biochemistry 2023Quote: ... the fraction was applied to a PD-10 column (GE Healthcare, IL, USA) to exchange the buffer for 50 mM ammonium acetate (pH 6.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) was used to determine the thickness of the VL of the right leg as previously described by our laboratory (18 ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred onto nitrocellulose membranes (Amersham Hybond, 10600021, GE Healthcare, Chicago, IL, USA) using Trans-Blot SD Semi-dry Transfer Cell system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: ... and transferred to nitrocellulose membranes (0.45 μm pore size; GE Healthcare, Chicago, IL). To evaluate the reactivity of each mAb ...
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bioRxiv - Physiology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare; Chicago, IL, USA) using a multifrequency linear-array transducer (L4-12T ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: HRP conjugated anti-mouse and anti-rabbit (GE Healthcare, Chicago, IL).
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bioRxiv - Immunology 2023Quote: Images were acquired using an INCell Analyser 2000 (GE Healthcare, Chicago, IL, USA) with the following settings ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% fetal bovine serum (FBS, Heat Inactivated, GE Healthcare, IL, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Heat-inactivated fetal bovine serum (FBS) was purchased from GE Healthcare (Chicago, IL). Bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... Radioactive probes were prepared by random-primed 32P labeling (GE Healthcare, Chicago, IL) of gel-purified DNA fragments of the following genes ...
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bioRxiv - Cell Biology 2024Quote: ... HRP activity was detected using ECL Prime reagents (GE Healthcare, Chicago, IL, USA) and captured using ImageQuant LAS 500 (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: Mononuclear cells were isolated from PB using Ficoll-Paque (GE Healthcare, Chicago, IL). Cryoconserved peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Biophysics 2019Quote: ... All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg, GE healthcare) pre-equilibrated with the buffer containing 20mM HEPES pH 7.5 and 150 mM NaCl (Supplementary Fig ...
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bioRxiv - Cell Biology 2019Quote: ... His-TWF-1 was further purified with Superdex-75 increase 10/300 GL gel filtration column (GE Healthcare) equilibrated 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... RAS:Affimer immunoprecipitation was as for RAS:RAF immunoprecipitation except His Mag Sepharose™ Ni2+-NTA beads (GE healthcare®) were used and incubated with 20 µg of Affimer K3 for 1 h with agitation ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to His-JMJD6 (1-343) was loaded to 7mL of Ni-NTA resin (GE Healthcare), washed with nickel-binding buffer containing 20mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Ni Sepharose High Performance (His Trap HP) and Superose 6 10/300 column were purchased from GE Healthcare.
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bioRxiv - Biochemistry 2021Quote: ... followed by gel filtration chromatography using Hi-Load 16/600 superdex 200 pg and 75 pg (GE Healthcare) on AKTA pure and AKTA prime systems ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Untagged His-GmPUB13 U-box and Avr1d were loaded on a superdex 200 gel-filtration column (GE Healthcare) and eluted in superdex buffer (20 mM Tris [pH 8.0] ...
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bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was incubated with His Mag Sepharose beads (equilibrated with lysis buffer according to manufacturer’s instructions; GE Healthcare) for 30 min at 4 °C rotating end-over-end ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... GST-tagged and His-tagged proteins were affinity-purified using glutathione agarose beads (GE Healthcare, Pittsburgh, PA, USA) and Ni-NTA affinity agarose beads (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... MS3_01370 was purified by IMAC by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...