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Citations for GE Life Sciences :
201 - 250 of 5464 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Bioengineering 2022Quote: ... gels were either silver stained (PlusOne Silver Staining Kit, Protein; GE Healthcare) or transferred onto nitrocellulose membranes for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated using the 2D Clean Up Kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Plant Biology 2021Quote: Total and virion DNA were amplified by rolling circle amplification (RCA) using the templiPhi100 DNA Amplification Kit (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... DNA was suspended in 50 µl of the hydration solution using a Tissue DNA isolation kit (GE HealthCare LifeSciences).
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bioRxiv - Immunology 2019Quote: Binding to FcγRs was carried out using a Biacore T200 biosensor (GE Healthcare). Recombinantly expressed FcγRS (R&D systems or Sino Biologicals ...
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bioRxiv - Biochemistry 2020Quote: Measurements of heparin binding were performed in a Biacore T200 instrument (GE Healthcare) using a heparin-coated chip (Xantec) ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2021Quote: ITC binding assays were carried out on a MicroCal ITC200 calorimeter (GE Healthcare) at 25 °C in a buffer containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: All analytical SEC binding assays were conducted on an ÄKTAmicro system (GE Healthcare) using a Superose 6 Increase 3.2/300 column (Cytiva ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted using the kit Illustrative Bacteria Genomic Mini Spin Kit (GE Life Sciences, Switzerland) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Microbiology 2021Quote: ... The ligated DNA templates were cleaned by an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, U.S.A). Finally ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNAs were extracted from blood samples and amplified using illustra GenomiPhi V2 DNA Amplification kits (GE healthcare Life Sciences).
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bioRxiv - Genetics 2019Quote: ... PCR products were cleaned with GFX Illustra DNA Purification Kit (GE Healthcare); standardized aliquots were sent to Macrogen (http://www.macrogen.com ...
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bioRxiv - Microbiology 2022Quote: ... and Illustra™ GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences) using 0.02 µm filtered PBS buffer as a sample ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were detected using enhanced chemiluminescence (ECL) detection kit (GE HealthCare, RPN2235).
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bioRxiv - Microbiology 2019Quote: Protein concentration was measured with the “2D Quant kit” (GE Healthcare Life Sciences), according to the supplier’s instructions ...