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Citations for GE Life Sciences :
1 - 50 of 5464 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... excess single-stranded DNA was removed using an S300 spin column (GE Healthcare). For the microscale thermophoresis (MST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was amplified using Illustra Single Cell GenomiPhi DNA Amplification Kit (GE Healthcare Life Sciences) according to the manufacturer’s protocol and purified using ethanol precipitation ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Developmental Biology 2019Quote: The single cycle kinetics method was used for sFLT1 binding assay by Biacore (GE Healthcare Bio-Science). A CM5 series S sensor chip (GE Healthcare Bio-Science ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Plant Biology 2022Quote: Leaf samples from ino-4 and ino-4 i20 plants were separately pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the provider’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Genomics 2020Quote: The total genomic DNA was extracted from a single aphid using Illustra Nucleon Phytopure kit modifying the manufacturer’s protocol (GE Healthcare). The lysis step was performed adding 10 ul of Proteinase K and incubating the sample in a water bath for 2 hours at 55°C ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... with precursor and reagent kits in single-use cassettes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting double-stranded DNA was then ligated into the BamHI/PstI-digested pRD2 vector using Ready-To-Go T4 DNA Ligase (GE Healthcare Life Sciences) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Genetics 2023Quote: ... Protein G Sepharose 4 Fast Flow (GE Healthcare, 17061801) was equilibrated with PBS containing 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... conjugated to Protein G SepharoseTM 4 Fast flow (GE Healthcare) and Protein A SepharoseTM 4 Fast flow (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...