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Citations for GE Life Sciences :
401 - 450 of 1195 citations for Human Neuropeptide FF NPFF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through fraction was further purified by HiTrap Q FF column and Superdex 75 16/60 column (GE Healthcare). The pooled fractions were concentrated to 20 mg/ml in crystallization buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer contained 500 mM imidazole and the eluted protein was immediately diluted with 20 mM Na-phosphate pH 6.5 followed by passing through an anion exchange chromatography column (HiTrap Q FF, GE Healthcare) and subsequent cation exchange chromatography (HiTrap SP FF ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 min and the supernatant was loaded onto a HisTrap FF crude column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 10 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and passed over a Q-sepharose FF (GE Healthcare) column ...
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bioRxiv - Biophysics 2022Quote: PTP1B WT* was purified initially via cation exchange with an SP FF 16/10 HiPrep column (GE Healthcare Life Sciences) in a lysis buffer (100 mM MES pH 6.5 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 7 ...
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bioRxiv - Biophysics 2023Quote: YidC and YidCΔC were purified from pTrc99a-YidC (Welte et al., 2012) and pTrc99a-YidCΔC expressing BL21 cells using a Ni-NTA FF crude column (GE Healthcare) on an ÄKTA chromatography system ...
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bioRxiv - Immunology 2023Quote: ... Minor contaminants were removed by passing the fractions through the HiTrap Q FF-Sepharose ion exchange column (GE Healthcare, UK) to remove any impurities ...
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bioRxiv - Neuroscience 2023Quote: ... The isolated inclusion bodies were solubilized in 6M GndHCl and purified on a 5mL FF Crude HisTrap column (GE Healthcare), equilibrated in binding buffer (2M GndHCl ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was then purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). The fractions containing α-Synuclein were dialyzed overnight against water and dried after aliquoting 200 ug into low binding Eppendorf tubes ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was further purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). Exclusively monomeric α- Synuclein was obtain by size exclusion chromatography using a HiLoad® 26/600 Superdex® 75 prep grade (from GE healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and loaded to HisTrap FF crude 5 ml Ni-NTA column (GE healthcare, #17-5286-01). Column was washed with 10 column volume of lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the clarified milk was thawed and loaded at 150 cm/h and 90 cm/h onto a 22 mL (1.6 x 11 cm) and 40 mL (1.6 x 20 cm) rPA Sepharose FF (GE Healthcare, Uppsala, Sweden) Protein A affinity chromatography column equilibrated with PBS for samples with a 25 mL and 100 mL milk starting volume ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was removed by centrifugation at 10000xg for 45 min at 4°C and the supernatant was loaded onto either a 1 mL or 5 mL HisTrap FF crude column (GE Healthcare) equilibrated in Ni-1 buffer using a peristaltic pump ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was purified using an AKTA Pure FPLC equipped with a HisTrap FF crude 5 mL column (GE Healthcare). Solvent A was 20 mM tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and was chromatographed sequentially on a 5-ml HiTrap Q HP column and on a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant obtained at this step was filtered through 0.45 μm filters and injected into a sample loop connected to HiPrep Q FF 16/10 (GE healthcare, 28936543). The supernatant was injected at 2 mL/min and eluted using buffer B (20mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was cleaved with 3C protease overnight and Nes2G-SR11/12 was further purified by cation exchange chromatography on a HiTrap SP-FF column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... The buffer was then adjusted to contain 40 mM imidazole and the rNAs were purified with a HisTrapTM FF 1 ml column (GE Healthcare) using an Akta Start protein purification system (Cytiva) ...
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The Pfkinesin-8B-MD construct retrieved from dialysis was loaded onto a 1ml HiTrap 1ml Q FF IEX column (GE healthcare). Pfkinesin-8B-MD_SNAP was eluted by gradient introduction of high-salt buffer (50mM Tris pH8.5 ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM MgCl2 was added and periplasmic extracts were harvested by centrifugation and the supernatant was loaded onto a 1 ml HisTrap FF column (GE Healthcare). The nanobody was eluted via a linear gradient into 400 mM imidazole ...
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bioRxiv - Molecular Biology 2019Quote: ... TcsL was purified by nickel affinity chromatography followed by anion exchange chromatography using HisTrap FF and HiTrap Q columns (GE Healthcare), respectively ...
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bioRxiv - Microbiology 2021Quote: ... and soluble supernatant was purified using HisTrap FF 5 mL immobilized metal ion affinity chromatography columns (GE Healthcare, New Jersey, USA) and eluted with 250 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates containing proteins with no His tag were purified by cation exchange chromatography (CIEX) using a 5 ml HiTrap Sepharose SP FF column (GE Healthcare) fitted on an ÄKTA fast protein liquid chromatography (FPLC ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The poly-histidine-tagged G3BP-1-WT as well as the mutated S149A and S149E constructs were purified using immobilized metal affinity (IMAC, HisTrap FF, GE Healthcare) and the dimer was isolated using size exclusion chromatography on a Superdex 75 column (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare). aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA ...
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bioRxiv - Pathology 2020Quote: ... the vector was purified from the cell lysate using a iodixanol density gradient followed by anion exchange chromatography (HiTrap Q-FF column, GE Healthcare). The scAAV9-CB-SMN vector was finally resuspended and concentrated in DPBS on a centrifugal filter unit (Amicon® Ultra-15 ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were lysed by three passes through an emulsiflex C5 (Avestin, Mannheim, Germany) and cleared cell lysate was loaded onto a HisTrap FF column (GE Healthcare). After washing with 10 column volumes of buffer A (30 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions containing LPR1 (detected by immune blot analysis) were directly applied to cation exchange carboxymethyl-sepharose column (HiTrap CM FF, 1-ml, GE Healthcare) equilibrated with buffer B (20 mM Na2HPO4-NaH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through fraction was further purified by HiTrap Q FF column and a Superdex 75 16/60 column (GE Healthcare). The pooled fractions were concentrated to 15 mg/ml in crystallization buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... IF3 was purified similarly to IF1 using a cation exchange chromatography on a HiPrep CM FF 16/10 column (28-9365-42, GE Healthcare), however using a stronger gradient in Buffer A (0.1 to 1 M NH4Cl) ...