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Citations for GE Life Sciences :
301 - 350 of 1195 citations for Human Neuropeptide FF NPFF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... PETISCO was purified by immobilized metal affinity chromatography (IMAC) using Ni2+-chelating beads (HisTrap FF; GE Healthcare). The HisTrap FF column was washed with 20 column volumes of lysis buffer and proteins were eluted with 20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... the HisTrap FF column was additionally coupled to a HiTrap Heparin HP 5-ml column (GE Healthcare). Both proteins were subjected to TEV protease cleavage at 4 °C overnight and another HisTrap FF 5-ml column purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... The second purification step involved ion-exchange chromatography using a 20ml HiPrep FF Q-column (GE Healthcare). The column was equilibrated and washed with buffer C ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were clarified by centrifugation and purified through a 5 mL HisTrap FF column (GE Healthcare), using theÄKTA Pure chromatography system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare) equilibrated in Buffer A (50 mM Hepes pH8 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was then subjected to IMAC purification by loading onto a HisTrap FF column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was loaded onto the pre-equilibrated HiTrap Q FF 5 mL column (GE Healthcare) at a flow rate of 2.5 mL/min ...
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bioRxiv - Immunology 2024Quote: ... filtered culture supernatant was purified with a 5 mL HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Cell Biology 2020Quote: ... The peak fractions were pooled and run on a nickel affinity column (HisTrap FF crude column, GE Healthcare) equilibrated with septin 2 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification was done using an AKTA Purifier FPLC system on a HisTrap FF 5 ml column (GE Healthcare). The column was washed and equilibrated with 10 volumes of LB prior to sample loading ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was subsequently loaded into an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences), pre-equilibrated in the SP buffer A to remove 6His-ZB tag and uncleaved protein ...
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bioRxiv - Microbiology 2020Quote: ... KCl was used instead of NaCl in all the buffers and a TALON FF crude column (GE Healthcare) was used for affinity chromatography.
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and His6-KIAA1841(BTB) was isolated on a HisTrap FF column (GE Healthcare). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2019Quote: ... filtered through a 0.22 µm filter and loaded onto a HiTrap IMAC FF 1 ml column (GE Healthcare) charged with Ni2+ and attached to an AKTA system ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell lysate was clarified by centrifugation and loaded onto a HisTrap FF column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to 5 or 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2019Quote: ... Harvested cells were sonicated for lysis and clarified lysate was loaded onto a HisTrap FF column (GE Healthcare). The His tag was cleaved by overnight incubation at 4 °C with SUMO protease (Invitrogen) ...
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bioRxiv - Synthetic Biology 2019Quote: The clean lysate was next loaded onto a HisTrap HP column (HisTrap FF crude 1 ml, GE Healthcare) at 4°C and flow rate of 0.5 ml min−1 ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was subjected to an affinity purification using a chelating HiTrap FF crude column (GE Healthcare) charged with Ni2+ ions on an AKTA-HPLC purifier (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on an affinity chromatography column (GE Healthcare, His-Trap FF crude, #17-5286-01) with a flow rate of 1 mL/ min ...
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bioRxiv - Genetics 2023Quote: ... for 45 min and soluble extract was applied to a 5 ml HisTrap FF column (GE healthcare Inc.) using a GE AKTA Avant 25 FPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed solution was then loaded on a 1 ml HisTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Cell Biology 2020Quote: The concentrated protein was then applied to a HiPrep 26/60 Sephacryl S-100 column (DEAE FF) (GE healthcare) which was equilibrated with the re-suspension buffer supplemented with 100 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... filtered through a 0.22 μm filter and loaded onto to a cation-exchange column (5mL, SP FF, GE Healthcare), pre-equilibrated with 30 mM MES ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was filtered (0.45 µm pore) and loaded onto a 5 ml HisTrap FF column (GE Healthcare, USA). After extensively washing with buffer NA supplemented with 35 mM imidazole ...
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bioRxiv - Synthetic Biology 2020Quote: ... the desalted protein-containing fractions were pooled and loaded on a Q sepharose FF column XK16/32 (GE Healthcare). The protein-containing fractions in the flow-through of the column were again pooled ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The lysate was cleared by centrifugation at 50,000 x g and filtered through a 0.45 µm filter before application to a HisTrap FF column (GE Healthcare). The column was washed with IMAC buffer (20 mM TRIS/HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was then filtrated (0.2 μm) and injected onto a Ni-NTA sepharose column (HisTrap FF, GE Healthcare) previously equilibrated with the lysing buffer ...