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Citations for GE Life Sciences :
151 - 200 of 4498 citations for Hepatitis E Virus Capsid Protein ORF2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Biophysics 2019Quote: ... The excess dye was removed by loading the capsid sample on a HiPrep Sephacryl 16/60 size exclusion column (GE Healthcare), using the appropriate buffer (for MS2 and MS2S37P ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Microbiology 2022Quote: ... and His-trap column (GE healthcare) previously equilibrated with Lysis Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was buffer exchanged with PBS and His-tagged proteins were captured with 5 mL HisTrap Excel columns (GE Healthcare), washed with 20 mM imidazole and eluted with 300 mM imidazole containing PBS buffer ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...