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Citations for GE Life Sciences :
101 - 150 of 4498 citations for Hepatitis E Virus Capsid Protein ORF2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was purified by TALON Superflow (GE Healthcare Life Sciences, Pittsburgh, PA, USA) according to the manufacturer’s description ...
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bioRxiv - Microbiology 2019Quote: ... The soluble His-tagged protein was purified using a HisTrap column (GE Healthcare Life Sciences, USA). Purity of the eluted protein was checked by SDS-PAGE gel staining with Coomassie Blue ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the protein without His6-tag was injected into a Superdex 75 10/300 GL (GE Healthcare) size exclusion column with the buffer B (50 mM NaH2PO4/Na2HPO4 pH 6.5 ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Microbiology 2019Quote: Purified capsids (100 µg) were loaded onto a 5-ml HiTrap™ Heparin-Sepharose HP Column (GE Healthcare), which then washed with Ca2+ and Mg2+-free PBS to remove unbound molecules and eluted with gradually linear increasing concentrations of NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... The CiEB1-mCherry proteins expressed with histidine tag were purified using a Ni-NTA column (GE Healthcare) and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Biophysics 2019Quote: ... All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg, GE healthcare) pre-equilibrated with the buffer containing 20mM HEPES pH 7.5 and 150 mM NaCl (Supplementary Fig ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... GST-tagged and His-tagged proteins were affinity-purified using glutathione agarose beads (GE Healthcare, Pittsburgh, PA, USA) and Ni-NTA affinity agarose beads (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleaved Cas9 protein was separated from fusion tag on HiLoad Superdex 200 16/60 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting tag-cleaved proteins were purified by ion exchange chromatography with a HiTrap SP column (GE Healthcare) and gel filtration with the final buffer (25 mM Tris-HCl buffer at pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-Trap Q (GE Healthcare), and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...