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Citations for GE Life Sciences :
401 - 450 of 4610 citations for HLA A*0201 AFP complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The complex was purified and analyzed by SEC-MALS with a Wyatt 050S5 column on an Ettan LC (GE Healthcare) in a running buffer containing 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Biochemistry 2022Quote: ... The H2A-H2B-APLFAD complex was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... the complex was further purified by anion exchange chromatography in presence of 2% CHAPS using the AKTA system (GE Healthcare), flash frozen and stored at −80 °C until use.
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bioRxiv - Plant Biology 2023Quote: ... SEP375–178 /AG90–189 complex was further purified by gel filtration using a Superdex 200 10/300 column (GE Healthcare). The protein complex was concentrated to 6-8 mg/ml and used for crystallization trials.
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bioRxiv - Biochemistry 2023Quote: ... The ternary complex was further purified by Strep-Tactin XT resin (IBA) and Superose 6 size exclusion chromatography (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Unbound iron was separated from the total complex using PD midiTrap-G25 columns following manufacturer’s instructions (GE Healthcare Bio-Sciences).
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to the elution peak were analysed by SDS-PAGE and those containing the TFIIIC complex were collected and loaded into a XK 16/70 Superose 6 pg column (GE Healthcare) equilibrated with 20 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... the purified membrane protein and nanobodies Nb1 and Nb2 were incubated at a 1:1.3:1.3 molar ratio for 30 min and the complex was further purified by size exclusion chromatography on a Superdex S200 column (GE Healthcare), pre-equilibrated in the same SEC buffer as used for EleNRMT purification ...
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bioRxiv - Microbiology 2019Quote: We first performed buffer exchange of the purified BamAB-RcsF complex using a PD-10 desalting column (GE Healthcare Life Sciences). The complex was eluted with 20 mM HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was then concentrated by reducing the volume from 48 ml to 1 ml using a 3 kDa cutoff concentrator and the hb complex was purified by SEC using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare). The identity of the hb complex peak was confirmed by SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2021Quote: The HTT-HAP40 complex was further purified by size exclusion chromatography (SEC) using a Superose 6 10/300 increase column (GE Healthcare) in running buffer 25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Nb was mixed with the target protein in 1.2-1.5 fold molar excess and the complex isolated via SEC on a Superdex 75 Increase 10/300 GL or Superdex 200 Increase 10/300 GL (GE healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... the complex between ARM and F15X was loaded on a size exclusion chromatography column HiLoad Superdex 16/600 200pg (GE Healthcare) equilibrated in a 25 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The complex was then purified on a Superdex 200 SEC column equilibrated with SEC buffer using an FPLC (AKTA, GE Healthcare). Some of the samples were treated with either EDTA or TPEN in order to chelate metal ions (Table I) ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified from excess/unassembled material using size-exclusion chromatography (HiLoad® 16/600 Superdex® pg200 (GE Healthcare) column running TBS buffer (Alfa Aesar)) ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescence was detected where binding occurred between SKI and PSMAD3–SMAD4 complex and measured with a Typhoon FLA 9500 biomolecular imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... 200 pmol of GST-RAC1 and 200 pmol of WAVE complex were mixed with 20 μL of Glutathione Sepharose beads (GE Healthcare) in 1 mL of binding buffer (10 mM HEPES pH 7 ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Molecular Biology 2021Quote: ... of the purified hPIV3 L-P complex was determined by analytical size-exclusion chromatography using a Superdex 200 Increase 5/150 GL column (GE healthcare) with buffer B ...
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bioRxiv - Biophysics 2021Quote: ... The LFT-μOR-Gi1-scFv16 complex was further purified by size exclusion chromatography on a Superdex 200 10/300 GL column (GE healthcare) in 20 mM HEPES 7.4 ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Biophysics 2019Quote: ... Purified Nup145NAPD was incubated with trimeric Nup821–854-Nsp1507–718-Nup1591072–1447 and the assembled tetrameric NPC-I complex separated by size-exclusion chromatography on Superdex S200 (GE Healthcare) 20 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing FluPolH5N1 complex were verified by SDS-PAGE gel and were further loaded onto size-exclusion chromatography (Superdex-200, GE Healthcare) in 20 mM HEPES (pH 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the Csx30–Csx31–RpoE complex was purified by Ni-NTA Superflow resin and a HiLoad 16/600 Superdex 200 column (GE Healthcare). The target RNA (25 nucleotides plus 5′ GG ...
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bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
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bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The REC114-MEI4 complex was purified using a Strep-Tactin XT resin (IBA) and by a gel filtration on Superdex 200 (GE Healthcare). For complex formation with IHO1 ...
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Biophysics 2023Quote: ... The 35S-methionine and 35S-cysteine incorporation into the core- and complex-glycosylated CFTR variants were visualized by fluorography and quantified using phosphorimage analysis on a Typhoon imaging platform (GE Healthcare). CFTR radioactivity was quantified after background subtraction ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Biochemistry 2024Quote: apoGAD65 and b96.11 Fab were purified as described and complex formation performed by mixing freshly purified GAD65 with 2X molar excess of b96.11 followed by incubation on ice for 15 min before subjecting the complex to analytical size exclusion chromatography using a Superdex 200 Increase 10/300 GL (GE Healthcare). The sample was used as is for negative stain EM ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was separated from Nb excess by size exclusion chromatography (SEC) on a Superdex-200 10/300 column (GE Healthcare). To concentrate we used protein concentrators with a cut-off of 100 kDa (Amicon ...
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bioRxiv - Biophysics 2020Quote: Hsc70 and MTB-Hsp70 were purified first by Ni-NTA column chromatography (His-trap, GE Healthcare). The column was washed with 50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... RSFPs were isolated from the supernatant with the His SpinTrap kit (GE Healthcare, Chicago, IL, USA) and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius ...
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bioRxiv - Biochemistry 2020Quote: ... The excessive imidazole was removed using a desalting column (Hi-Prep™ 26/10, GE Healthcare). The 8 × His-SUMO was cleaved by TEV proteinase and the cleaved fragment was removed through the affinity of the His-tag to a Ni-NTA column ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a heparin column (Hi-trap Heparin HP, GE Healthcare Life Sciences) and MatP was eluted with a NaCl gradient (0.3 to 1M ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...