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Citations for GE Life Sciences :
351 - 400 of 4610 citations for HLA A*0201 AFP complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the GST-PACRGΔ1-69:MEIG1 complex was purified by affinity chromatography using 1.5 mL of glutathione-sepharose resin (GE Healthcare) washed in Buffer A (50 mM of HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... and loaded onto gel filtration (Superdex S75 for ISCU and FXN, Superdex S200 for NFS1-ISD11 complex; GE Healthcare). Peak fractions were collected ...
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bioRxiv - Biochemistry 2021Quote: ... and the complex was purified by gel filtration on a Superdex 200 10/300 global increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... the complex was centrifuged at 17,000 xg for 10 minutes and then loaded onto a Superose 6 (GE Healthcare) column equilibrated in wash buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The eluted zRGα1a complex was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM HEPES (pH 7.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled transcribing Pol II complex was then applied onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated with the SEC150 buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The final clamp loader complex was purified on the HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare). The purified clamp loader was concentrated to ∼ 10 mg/mL and stored in 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... and the complex was further purified by size-exclusion chromatography (Superdex 200 increase 10/300, Cytiva, formerly GE Healthcare), eluting with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Biochemistry 2023Quote: ... The binary complex was fractionated by SEC using a HiLoad Superdex 75 26/60 column (GE Healthcare, Piscataway, NJ) in 25 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... Concentrated apo-KSHV-GPCR-Gi complex was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Molecular Biology 2021Quote: 1ug of soluble His-Rap1 was pre-cleared on Glutathione sepharose 4B beads (GE Healthcare) for 1h at 4C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1mL His-Trap FF column (GE Healthcare Life Sciences) and ZapB was eluted with an imidazole gradient (0 to 0.5M) ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated samples were loaded onto Hi Load 16/600 Superdex 75 pg column (GE Healthcare) connected to the NGC Scout Chromatography system and running buffer composed of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was applied to a Hi-Load Superdex 200 16/60 column (GE Healthcare) in a buffer A ...
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bioRxiv - Microbiology 2020Quote: ... And further purified by gel filtration on a Hi Load Superdex 75 column (GE Healthcare). Peak fractions were concentrated to 10 mg/ml in 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant (crude extract) loaded on a 1-ml His-Trap column (GE Healthcare) previously equilibrated with Buffer H ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was then loaded onto a His-Trap column (GE Healthcare Life Sciences) and eluted with buffer B (100nM NaCl and 500 mM imidazole in 50 mM Tris at pH 8.0) ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was then applied to a nickel-affinity chromatography (His Trap excel, GE Healthcare) equilibrated with buffer B (20mM Tris pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... was purified by IMAC on a Ni2+-His-Trap column (GE Healthcare, Chicago, IL, USA) in buffer 0,1M K-phosphate pH 7,5 ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Biochemistry 2022Quote: ... and the filtrate was loaded on to a Hi Trap SP HP column (GE healthcare) equilibrated in buffer B1 [50 mM Na2HPO4 at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on an equilibrated 5 mL His-trap column (GE healthcare/Cytiva) at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... Thawed cells were spun and Ku was bound to His Mag Sepharose Ni (GE Healthcare). Beads were washed in Lysis buffer containing 50 mM imidazole and Ku was eluted in Lysis buffer with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then passed through a 5 mL His-Trap FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the his-tagged FABP1 was purified using HisTrap HP affinity column (GE Healthcare, Chicago, IL), the tag was cleaved by thrombin and the cleaved protein was purified and buffer exchanged by gel filtration into 10 mM potassium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... the samples were loaded on a Hi-Load Superdex 200 16/600 column (GE Healthcare) equilibrated with 50mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Biophysics 2021Quote: ... The complex was separated from excess Lf using SEC on a Superdex 200 10/300 GL Increase column (GE Healthcare). Complex formation was confirmed using SDS-PAGE gel ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was further purified by size exclusion chromatography (SEC) on a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in SEC Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was purified by size exclusion chromatography (SEC) on a Superdex S200 Increase 10/300 GL column (GE Healthcare) into buffer comprised of 100 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The METTL3- METTL14 complex was further purified by successive passage through HiTrap heparin and HiLoad Superdex 200 columns (GE Healthcare). The protein complex was eluted as a single homogenous species in a final buffer containing 0.02 M Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... and then separating the complex from free GTPase by a HiLoad 26/60 Superdex 200 gel filtration column (GE Healthcare), followed by a RESOURCE™ Q column ...