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Citations for GE Life Sciences :
1 - 50 of 4962 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... PDIs and their mutants were separately reduced with 10 mM DTT for 10 min at 30°C followed by DTT removal through a PD-10 column (GE Healthcare) pre-equilibrated with 50 mM Tris/HCl (pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... ultra-centrifuged (280.000 x g, 10 min, 4°C) and gel-filtered using a Superose6 10/300 GL column (GE Healthcare). Peak fractions were pooled ...
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bioRxiv - Molecular Biology 2022Quote: ... for 10 min at 4 °C and injected into Superdex 200 Increase 10/300 GL size-exclusion columns (GE Healthcare). Fractions containing both hZnT7 and Fab were collected and concentrated with a 100-kDa cutoff Amicon filter to around 10 mg ml-1 for cryo-EM measurements.
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... C monoclonal antibody (W6/32) coupled Sepharose-4B resin (GE Healthcare, IL, USA) at room temperature for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The membrane was incubated first with primary antibodies for 24 h at 4°C and then with horseradish peroxidase– conjugated secondary antibodies (GE Healthcare) for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... and then incubated with primary antibodies overnight at 4°C and then again with horseradish peroxidase–linked secondary antibodies (GE Healthcare) in PBST with 5% dry milk for 1 hour at RT ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The chromatography was performed at 25 °C using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with Dialysis Buffer ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Separation was performed at 20°C using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with a flow rate of 0.5 mL min−1 and a mobile phase consisting of 20 mM Na-HEPES pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then separated in 10% SDS polyacrylamide gels and transferred to Hybond C nitrocellulose membranes (GE Healthcare). After blocking with 5% non-fat milk in PBS/0.05% Tween ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Analytical size exclusion chromatography was performed at 24 °C using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a mobile phase containing 10 mM HEPES (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Microbiology 2023Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Cancer Biology 2019Quote: ... The clicked antibodies were purified by SEC (Superdex 200, 10×300 GL, GE Healthcare) at a flow rate of 0.5ml/min in PBS using a GE AKTAPurifier ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The complex was incubated for 10 min at 22°C before size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) at 22°C ...
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bioRxiv - Biochemistry 2020Quote: ... After the reaction, the sample was centrifuged briefly (10 min, 16,000g, 4°C) and loaded onto a Superdex 200 column (GE Healthcare) to remove Sfp and unreacted dsOligo-CoA ...
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bioRxiv - Plant Biology 2020Quote: ... An equal amount of protein was boiled in Laemmli buffer for 10 min at 95°C and separated on 12.5% acrylamide gel and blotted on a nitrocellulose membrane (GE healthcare). Blocking was done with 0.5% w/v of BSA in Tris-buffered saline with 0.1% v/v Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2022Quote: ... filter and size-exclusion chromatography at 4 °C on a Superdex 200 10/300 GL gel filtration column (GE Healthcare). Purity of the proteins was evaluated on SDS-PAGE gel ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biochemistry 2019Quote: ... Extracts were cleared by centrifugation for 10 min at 21,000 × g at 4°C and incubated while rotating with 200 μl IgG Sepharose beads (GE Healthcare) for 4 hrs at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting supernatant was recovered and centrifuged at 10,000 xg for 10 min at 4°C and then loaded onto a HisTrap column (GE Healthcare) pre-equilibrated with the same resuspension buffer ...
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bioRxiv - Plant Biology 2022Quote: Analytical size exclusion chromatography was performed at 4°C using a Superdex 75 10/300 gel filtration column (GE Healthcare) pre-equilibrated in 20 mM HEPES pH 7.5 and 150 mM NaCl (Maqbool et al ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent ...
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Molecular Biology 2022Quote: ... to approximately 3 – 4 ml and further purified by size-exclusion chromatography at 18 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare). His-TEV-CobB ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...