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Citations for GE Life Sciences :
351 - 400 of 4962 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... and RBM3 (FliF231-438) were applied to a Superdex 200 10/300 column and to a Superose 6 10/300 column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2021Quote: All the gel filtration chromatograms were ran in a Superdex 200 increase 10/300 column (fractionation range 10-600 kDa, GE Healthcare) coupled to an AKTA purifier ...
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bioRxiv - Biochemistry 2019Quote: ... The crude GAG extract was re-suspended in 1 mL of HPLC grade water and loaded onto a pre-packed DEAE-Sephacel column (10 mm I.D. x 10 cm; GE Healthcare, UK) at a flow rate of 1 mL.min-1 ...
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bioRxiv - Biochemistry 2020Quote: ... We purchased all columns (26/10 HiPrep [desalting], HisTrap HP [Ni], and HiPrep Q HP 16/10 [anion exchange]) from GE Healthcare, Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Nb was mixed with the target protein in 1.2-1.5 fold molar excess and the complex isolated via SEC on a Superdex 75 Increase 10/300 GL or Superdex 200 Increase 10/300 GL (GE healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superpose 6 In-crease 10/300 GL or Superdex 200 Increase 10/300 GL; GE Healthcare) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The relevant fractions were pooled and concentrated to 10-20 mg/mL for loading on a Superdex 200 Increase 10/300 (GE Healthcare) size exclusion chromatography column equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Ni2+-NTA eluted fractions containing the polyprotein were concentrated to 10 mg/mL and further purified over a 10/300 Superdex 200 column (GE healthcare) in 20 mM phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Microbiology 2020Quote: ... the NP and 1TD0-derived epitope probes were further purified by SEC on a Superose 6 10/300 GL column and a Superdex 75 10/300 GL column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2020Quote: Analytical gel filtration was performed on a Superdex 200 Increase 10/300 GL column or Superose 6 Increase 10/300 GL (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... was done by connecting of either a Superdex 200 Increase 10/300 or a Superdex 75 10/300 (both GE Healthcare) to a UV-Vis Prostar 335 detector (Varian ...
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bioRxiv - Biochemistry 2020Quote: ... and fl-SNED1 (4.5 μM) were injected on Superdex 75 Increase 10-300 GL and Superdex 200 Increase 10/300 GL columns (GE Healthcare) respectively at a flow rate of 0.5 ml/min at 20°C ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were subjected to size exclusion chromatography using either a Superdex 200 10/300 or Superdex 75 10/300 column attached to an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... then UCN1-CRF2R-Go and UCN1-CRF2R-G11 were loaded onto a Superdex200 10/300 GL column and Superose6 Increase 10/300GL (GE Healthcare), respectively ...
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bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then injected at a 0.4 mL/min flow rate onto a Superdex 200 GL 10/300 (or in the case of Figure S4, Superdex 200 Increase GL 10/300) SEC column (GE Healthcare) using HSEC buffer with 1 mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated protein sample was centrifuged at 10,000 × g and 4°C for 10 min before the supernatant was loaded onto a lysis buffer equilibrated Superose 6 increase 10/300 GL column (GE Healthcare), connected to a 1260 Bioinert Infinity LC system and equipped with a multi-angle light scattering detector (miniDawn 3141MD3 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: Enzymatic degradations were monitored by analytical gel permeation chromatography using Superdex S200 10/300 and Superdex peptide 10/300 (GE Healthcare) columns mounted in series and connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Biophysics 2023Quote: ... and followed by size-exclusion chromatography (SEC) using a superdex 200 Increased 10/300 chromatography column on an AKTA Purifier 10 FPLC system (GE Healthcare) with buffer containing 20 mM Hepes ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma#255599) ...
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bioRxiv - Cell Biology 2021Quote: ... Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare): Hybridization was performed overnight in the same buffer containing a 32P-labeled probe ...
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bioRxiv - Genomics 2020Quote: Widefield fluorescence imaging was performed at 20 °C on a DeltaVision Elite system (Applied Precision) equipped with a 100 ×/1.40 NA UPLSAPO oil immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... SPR was performed at 25°C using Biacore T200 system with CM5 chips (GE Healthcare). HBS-T (10 mM Hepes ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The binding study was carried out at 25°C in PBS-P buffer (GE Healthcare). The heparin molecule or fondaparinux at different concentrations were flowed over the immobilized recombinant proteins at a flow rate of 30 μl/min with a contact time of 60 s and a dissociation time of 100 s ...
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bioRxiv - Biochemistry 2020Quote: ITC experiments were carried out at 5°C with a MicroCal iTC200 instrument (GE Healthcare). Each of the ATAD2B bromodomain proteins and histone peptide samples were dialyzed into ITC buffer containing 20 mM NaH2PO4 pH 7.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: SPR measurements were carried out at 25°C using a Biacore X instrument (GE Healthcare) as described previously28 ...
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bioRxiv - Genetics 2019Quote: Widefield fluorescence imaging was performed at 20°C on a DeltaVision Elite system (Applied Precision) equipped with a 100 × /1.40 NA UPLSAPO oil immersion objective (Olympus) ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Neuroscience 2022Quote: Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS + 0.005% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred onto PVDF membranes (Hybond-C Extra, GE Healthcare Life Sciences, Mississauga, ON, Canada). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Microbiology 2024Quote: ... ITC experiments were performed at 25°C using an ITC-200 MicroCal microcalorimeter (GE Healthcare) following a standard procedure (Hands-Taylor et al ...
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bioRxiv - Bioengineering 2024Quote: Kinetic analyses were performed at 25°C using a BIAcore 3000 machine (GE Healthcare Technologies). A2p1 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated anti-rabbit IgG donkey antibody and HRP-conjugated anti-goat IgG donkey antibody (GE Healthcare), HRP-conjugated Protein-G (abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL or Superose 6 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples at concentrations >10 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were concentrated with 30 kDa cutoff concentrators (Amicon) to ∼10 mg/ml and loaded onto a S200 Increase 10/300 GL (GE Life Sciences) in SEC Buffer [20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...