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Citations for GE Life Sciences :
101 - 124 of 124 citations for Dehydroepiandrosterone sulfate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Hybond ECL membrane (Amersham Biosciences, GE Healthcare). Membrane was stained with Ponceau S solution (Sigma-Aldrich).
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bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts of protein extracts were electrophoresed on 12% sodium dodecyl sulphate (SDS) polyacrylamide gel prior to transfer on Hybond ECL nitrocellulose membranes (GE Healthcare). Homogenous transfer was confirmed by Ponceau Red staining ...
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bioRxiv - Genomics 2024Quote: ... Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare). The membranes were incubated with primary antibodies in a blocking solution (2% BSA in PBS containing 0.1% Tween 20 (PBS-T) ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Microbiology 2021Quote: Whole cell protein lysate from 1 × 106 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were blocked by incubation with 5% milk powder in PBS for 1 h at room temperature (RT ...
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bioRxiv - Microbiology 2022Quote: Fresh blood was collected from healthy donors in a sodium heparin vial and PBMCs were isolated by Ficoll-paque™ PLUS (GE healthcare) density gradient centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate, pH 7.2, 500 mM NaCl, 30mM imidazole) using an AKTA FPLC (GE Healthcare, Piscataway, NJ). The column was washed with 15 column volumes of binding buffer and protein was eluted in a linear gradient to 100% elution buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... FhuFred was prepared using sodium dithionite and then its excess was removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). The different concentrations were used to avoid and deal with the spectra overlap between FhuF and the Fe(III)-siderophores ...
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bioRxiv - Cell Biology 2023Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Molecular Biology 2023Quote: ... with 20 mM sodium phosphate (pH 6.5) and by size-exclusion chromatography on a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Sciences) with 20 mM sodium phosphate (pH 6.5 ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µg of freeze-dried HMW compounds were dissolved in 1 mL of 20 mM sodium acetate buffer (pH 4.5) and eluted through a 1 ml HiTrap ANX FF column (GE Healthcare, 17-5162-01). Samples were eluted at a flow rate of 1 ml/min with 20 mM sodium acetate buffer pH 4.5 for 36 ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... They were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – #SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and were cultured in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
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bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...