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Citations for GE Life Sciences :
1 - 50 of 124 citations for Dehydroepiandrosterone sulfate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 20 mg/ml sodium dodecyl sulfate (SDS, GE Healthcare), 40 nM N-ethylmaleimide (NEM ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5% Sodium dodecyl sulfate (SDS; GE Healthcare, cat. no 17-1313-01), 8% glycerol (Alfa Aesar ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins (20 μg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (10600023, GE Healthcare). After transfer ...
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bioRxiv - Microbiology 2022Quote: ... The resulting translated proteins were resolved by a sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) gel and analyzed by phosphoimager analysis (Typhoon, Amersham, GE life sciences).
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (10600023; GE Healthcare Life Sciences, Chalfont, UK). After blocking with 5% skim milk (232100 ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... disposable PD10 desalting salt columns were purchased from GE Healthcare Life Sciences and Amicon® Ultra centrifugal filters from Merck Millipore.
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M ammonium sulfate) and fractionated on a DEAE Sephacel column (GE Healthcare). The flow-through fractions were further fractionated on a phosphocellulose 11 column ...
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Immunology 2019Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was injected on HiTrap Chelating HP column loaded with nickel sulfate (GE Healthcare). Proteins were eluted by an imidazole gradient using a fast protein LC Äkta System (GE ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X Hank’s Balanced Salt Solution (SH30588.02, GE Healthcare Life Sciences, United States). The dura was removed and the spinal cord was bisected lengthwise ...
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bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was eliminated by a desalting column step (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
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bioRxiv - Biochemistry 2023Quote: ... Ammonium sulfate was removed by injection on a desalting column (HiPrep 26/10 desalting, GE Healthcare; ÄKTA Avant). The sample was concentrated before separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Physiology 2019Quote: ... supplemented with 1 mM sodium pyruvate (GE Healthcare, Thermo-Fisher), 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled and supplemented with ammonium sulfate to a final concentration of 1.5M and loaded on a 5ml pre-packed HiTrap Phenyl HP column (GE Healthcare) equilibrated with buffer A (100mM Tris pH 7.3 ...
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bioRxiv - Biophysics 2019Quote: ... Salt was removed from the eluted samples with Vivaspin 500 ultrafiltration system (MW cutoff: 3000; GE Healthcare), and samples were amplified by PCR using the primers from the DNA library (0.25 mM forward primer and 25 mM reserve primer) ...
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bioRxiv - Biochemistry 2021Quote: ... The phosphorylation mixture was then dialyzed against low salt buffer and loaded to a MonoS (GE HEalthcare) column ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...
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bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Myristoylated Arf1 was enriched by ammonium sulfate precipitation (35% saturation) then applied to HiLoad 16/60 Superdex 75 column (GE Healthcare) in 20 mM Tris at pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... and mi3 particles were isolated by ammonium sulfate precipitation followed by SEC purification using a HiLoad 16/600 Superdex 200 (GE Healthcare) column equilibrated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% dextran sulfate) at 65°C probed with 32P-labeled CLB3 DNA probes prepared via Amersham Megaprime DNA labeling kit (GE Healthcare) and Illustra ProbeQuant columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions eluted from anionic exchange column were incubated with ammonium sulfate to a final concentration of 1M and loaded on a Butyl Sepharose High Performance HiLoad16/10 (GE Healthcare) equilibrated with buffer C (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... after which the reaction mixture was passed through the salt exchange column (HiPrep 16/10 Desalting, GE Healthcare) with FPLC UV filter set at 254nm and then concentrated using 10kDa concentrator ...
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bioRxiv - Neuroscience 2024Quote: ... diluted twice to lower salt concentration to 50 mM and bound to a MonoS Sepharose column (GE Healthcare). Finally ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 150 mM sodium chloride (HBS) and loaded onto protein G resin (GE Healthcare) equilibrated with HBS ...
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bioRxiv - Neuroscience 2019Quote: ... the supernatant was passed through a 0.22 µm sodium acetate filter (GE Healthcare). The resulting flow-through (filtered ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Cell Biology 2020Quote: ... Umbilical cords were flushed with Hank’s balanced salt solution with magnesium and calcium (HyClone™, GE Healthcare, Solingen, Germany) and PBS with magnesium chloride and calcium chloride (Merck KGaA ...
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bioRxiv - Microbiology 2020Quote: ... the redundant salt in mixture was removed in a G25 desalting column (GE Healthcare Life Sciences, Pittsburgh, PA, USA) equilibrated with 50 mM HEPES solution (pH 7.3).
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bioRxiv - Biochemistry 2022Quote: ... The dissolved ammonium sulfate pellet was loaded on a 5 mL DEAE Sepharose and 5 ml Ni-Sepharose cartridge (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...