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Citations for GE Life Sciences :
1 - 50 of 134 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cy3 or Cy5 amine-reactive dyes (Amersham CyDye Mono-Reactive Dye Packs, GE Healthcare) were dissolved in 20 µL DMSO ...
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bioRxiv - Microbiology 2022Quote: ... Enough Cy5 monoreactive dye (GE Healthcare) to label 1 mg protein was added to 3-4 mg toxin and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... following labeling with Cy3 dye (GE Healthcare). Following incubation in the dark at 27°C for 20 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cy3 mono-Reactive Dye Pack (GE HealthCare), and Alexa Fluor 647 Carboxylic Acid succinimidyl Ester (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and Cy3 and Cy5 dyes (GE Healthcare) following standard protocols for Agilent array-CGH (CGH enzymatic protocol v6.2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cy3 mono-Reactive Dye Pack (GE HealthCare), and Alexa Fluor 647 Carboxylic Acid succinimidyl Ester (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Cy3 or Cy5 NHS-dyes (GE Life Sciences) were dissolved in DMSO and added directly into the RNA-lysine solution mentioned above (final dye concentration ~ 500 μM from 10 mM stocks) ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes from Qiagen were labeled with Cy3 monoreactive dye (GE Healthcare) and purified by ethanol precipitation and washing with 80% ethanol until the supernatant was colorless ...
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bioRxiv - Immunology 2021Quote: ... and labelled with Cy3 monoreactive dye pack (GE Healthcare) in sodium carbonate buffer (biotinylated anti-κ-Cy3) ...
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bioRxiv - Neuroscience 2019Quote: ... and Cy3 mono-reactive dye pack (GE Healthcare, #PA23001), conjugated to affinity-purified secondary antibodies from Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... using the NHS-ester form of Cy dyes (GE Healthcare). 2012).1 μL of 1 mM of DNA/RNA dissolved in MilliQ H20 is added to 5 μL labeling buffer of (freshly prepared ...
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bioRxiv - Biophysics 2020Quote: ... Excess dyes was removed by HiTrap desalting column (GE Healthcare) in buffer C ...
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bioRxiv - Biochemistry 2023Quote: ... Cy5 monoreactive dye was obtained from GE healthcare (Buckinghamshire, UK). C18 column (5 μm ...
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A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integrationbioRxiv - Molecular Biology 2024Quote: NusA was labeled with Cy5-maleimide dye (PA25031, GE Healthcare) using an 8:1 molar ratio of dye to protein in a total volume of 200 µL (∼20 µM protein ...
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bioRxiv - Microbiology 2020Quote: ... were labeled using the Cy5 monoreactive Dye pack (PA25001, GE Healthcare) and filtered using Illustra microspin G25 columns (27532501 ...
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bioRxiv - Biophysics 2022Quote: ... Unconjugated dye was removed by desalting with Sephadex G50 (GE Healthcare) followed by dialysis.
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bioRxiv - Immunology 2020Quote: Conjugation of CLTX (Alomone Labs) with Cy5.5 fluorescent dye (GE Healthcare) or biotin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The free dye was removed using a NAP column (GE Healthcare). The concentration of dye and protein were determined by measuring absorbance at 488 and 280 nm ...
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bioRxiv - Biochemistry 2020Quote: ... Free dye was removed using a PD-MidiTrap column (GE Healthcare) and Amicon Ultra-15 Centrifugal Filter Units in a buffer containing 20mM HEPES pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... an Amersham Cy3 mono-reactive NHS ester dye pack (GE healthcare) was resuspended with labeling buffer and mixed with enough purified APE1 to yield a 100 μL solution of 10 mg/mL APE1 ...
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bioRxiv - Microbiology 2021Quote: ... free dye was removed using a PD 25 MiniTrapTM (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The Cy3/Cy5-maleimide dyes are purchased from GE Life Sciences. The labeling efficiency is approximately 50% based on spectrophotometer absorptions.
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bioRxiv - Biophysics 2020Quote: ... 5-fold molar excess of maleimide fluorescent dyes Cy3B (GE Healthcare), AF555 (Life Technology ...
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bioRxiv - Immunology 2020Quote: ... Free-unlabeled dye was removed by PD-10 columns (GE healthcare) and concentrated using Amicon® Ultra Centrifugal Filters (Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... Excess dye was removed with G25-PD10 desalting column (GE Healthcare) and MGL fractions dialyzed further against PBS buffer and concentrated ...
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bioRxiv - Biophysics 2023Quote: ... Excess dye was removed by desalting via PD10 columns (GE Healthcare) before reconstitution.
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bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... free dye was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer (5 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... a cAMP to which the fluorescent dye Cy3B (GE Healthcare, Frankfurt, Germany) was linked via an aminohexylthio spacer to position 8 of the adenosine moiety (64 ...
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bioRxiv - Biophysics 2020Quote: ... Excess dye was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer (20 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Excess unconjugated dye was removed using PD-10 desalting columns (GE Healthcare). pHrodo Red-labeled antibodies were exchanged into HEPES buffer and concentrated in an Amicon Ultra centrifugal filter unit with a nominal molecular weight cutoff of 30 kDa ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... an excess of Cy3b-NHS dye (50 μg, GE Life Sciences, # PA63101) was dissolved in DMSO and added to a solution containing 10nmol of the DNA ligand strand in 1xPBS with 0.1M NaHCO3 ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were incubated with Quickstain Cy5 protein-dye (GE Healthcare Bio-Sciences) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Excess dye was removed by Superdex 75 column (10/300 GL, GE Healthcare). Percent labeling was calculated by measuring absorbance at peak excitation for the dye and 280 nm for the protein ...
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bioRxiv - Biophysics 2020Quote: ... The unbound dye was removed by using a PD-10 column (GE Healthcare). The final protein and probe concentrations were determined spectrophotometrically ...
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bioRxiv - Biophysics 2022Quote: ... Excess fluorescent dyes were removed by a PD-10 desalting column (GE Healthcare) with buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Excess fluorescent dyes were removed by a PD-10 desalting column (GE Healthcare) in buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Lectins were labeled with Cy5 mono-reactive dye (NHS ester) from GE Healthcare. Briefly ...
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bioRxiv - Biophysics 2020Quote: ... Stochastic labelling was performed with the maleimide derivative of dyes Cy3B (GE Healthcare) and ATTO647N (ATTO-TEC ...
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bioRxiv - Molecular Biology 2019Quote: ... Probes were labeled with Cy3 or Cy5 Mono-Reactive Dye Pack (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The unreacted dye was removed on a desalting column (Sephadex G25, GE Healthcare). The protein was exchanged into buffer containing guanidinium chloride in order to fully expose unlabeled cysteine residues (25 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Free dye was separated from labelled protein by Illustra NICK columns (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2019Quote: ... Free dyes were subsequently removed by a Sephadex G-25 column (GE Healthcare). Nucleosomes were assembled by salt gradient dialysis as previously described (Lee and Narlikar ...
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bioRxiv - Biophysics 2019Quote: Stochastic labelling was performed with the maleimide derivative of dyes Cy3B (GE Healthcare) and ATTO647N (ATTO-TEC ...
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bioRxiv - Cell Biology 2021Quote: ... Uncoupled dye was removed using a PD-10 gel filtration column (GE Healthcare). Fluorescence polarisation measurements were recorded on a BMGLabTech CLARIOstar plate reader and analysed using GraphPad Prism (version 6.07) ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Biophysics 2023Quote: ... free dye was removed using a spin column (PD 25 MiniTrap, GE Healthcare) preequilibrated with measurement buffer (40 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Uncoupled dye was removed using a PD-10 gel filtration column (GE Healthcare). Fluorescence polarization measurements were recorded on a BMGLabTech CLARIOstar plate reader and analysed using GraphPad Prism (version 6.07) ...