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Citations for GE Life Sciences :
401 - 450 of 2258 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were isolated and purified by Percoll density gradient (72%, 63%, 54%, and 45%) (GE Healthcare; Cat. 17-5445-01). Isolated neutrophils were resuspended in RPMI 1640 (Corning ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtrated through 0.45 µm filter and loaded into HisTrap HP column (GE Healthcare, Cat#:17-5248-02). The collected fractions were concentrated by Amicon® Ultra-15 Centrifugal Filter Units (EMD Millipore ...
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bioRxiv - Microbiology 2022Quote: Neutrophils were purified by centrifugation for 30 min at 1,300xg on a discontinuous Percoll gradient (GE Healthcare #17-0891-01) consisting of 62% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Immunology 2023Quote: ... resuspended in HBSS and 20mM HEPES and loaded onto a 62% (v/v) Percoll gradient (GE Healthcare #17-0891-01) in HBSS and 20mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutants were expressed in BL21 as a standard procedure and then purified with glutathione-sepharose (GE Healthcare, 17-0756-01) as previously described [13] ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: Whole blood was collected in heparin tubes and PBMCs were isolated by Ficoll-Paque (GE Healthcare, Cat: 17-1440-03) gradient centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction solution was sequentially precipitated using protein A sepharose beads and Glutathione Sepharose beads (#17-0756-01, GE Healthcare). The resulting supernatant was stored at −20 °C until it was analyzed for the surface plasmon resonance (SPR ...
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bioRxiv - Genomics 2023Quote: ... and lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were isolated from cleared lysates by incubating with 70 µl of streptavidin beads (17-5113-01, GE Healthcare) at 4°C for 3h ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389 ...
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bioRxiv - Biophysics 2021Quote: ... Excess reactants were removed by a Superdex peptide size-exclusion column (GE Healthcare) equilibrated in the refolding buffer ...
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bioRxiv - Biophysics 2019Quote: ... Solutions of the SGYDFEREGYS and SGYDFGREGYS peptides in PBST buffer (GE Healthcare, USA) at the indicated concentrations were used as analyte ...
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bioRxiv - Biochemistry 2020Quote: Peptides were fractionated by SCX chromatography using the AKTA Purifier system (GE Healthcare) and injected for nanoLC-MS/MS analysis ...
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bioRxiv - Biochemistry 2021Quote: ... The QconCAT peptides were purified with Ni Sepharose suspension (GE Healthcare Ltd, UK) in centrifuge filters (Corning Costar Spin-X 0.45 um pore size cellulose acetate membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescently labeled peptides were detected using a Typhoon Trio Plus imager (GE Healthcare) and band intensities were quantified using the ImageJ44 ...
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bioRxiv - Cell Biology 2023Quote: ... Free Flag peptides were removed using a PD-10 Desalting Column (GE Healthcare). The conjugate was further purified by a new PD-10 Desalting Column ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptides were desalted using a homemade C18 microcolumn (SOURCE 15RPC, GE Healthcare) and stored at -30 °C until use.
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and loaded to HisTrap FF crude 5 ml Ni-NTA column (GE healthcare, #17-5286-01). Column was washed with 10 column volume of lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a tube containing 30 µl of pre-washed packed streptavidin beads (GE Healthcare, 17-5113-01). Streptavidin pulldown was done for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Genomics 2019Quote: ... Spheroplasts were lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Biophysics 2021Quote: ... the sample was filtered through a 0.22 μm filter and then purified by the Q column (GE Healthcare, 17-5156-01) and the Superdex 75 column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble high-molecular weight seeding-competent tau (HMW SEC tau) was isolated from homogenate using size exclusion chromatography on a Superdex200 10/300GL column (#17-5175-01, GE Healthcare) as described previously(Takeda et al. ...
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bioRxiv - Microbiology 2021Quote: ... Lithium heparin-coated tubes were used for blood collection and plasma was isolated using Ficoll-Hypaque (GE Healthcare; 17-1440-03) in accordance with manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Gels were dried and exposed to PhosphorImager screens overnight (approximately 17 hours) and imaged with a Typhoon-9410 scanner (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from the patient samples and healthy donor were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare). The isolated PBMC were stored by resuspending cells in cryopreservation media in aliquots of 5-10×106 cells per ml and storaed on liquid nitrogen.
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bioRxiv - Neuroscience 2022Quote: ... Samples were divided into two equal parts and added with 50 μl pre-equilibrated thiopropyl Sepharose 6B (Cat#17-0420-01, GE Healthcare) respectively ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Microbiology 2023Quote: ... This suspension was slowly added to the top of 4 mLFicoll-Paque density gradient (GE Healthcare #17-1440-02, Uppsala, Sweden) and centrifuged for 40 minutes at 400 x g ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation using a Ficoll-Paque Plus gradient (GE Healthcare, Cat #17-1440-02), then washed by centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant GST-RAPSYN was purified from the supernatant by GST-affinity chromatography (SA010010, Smart-Lifescience) and size-exclusion chromatography (17-0060-01, GE Healthcare). Purified GST-RAPSYN protein was digested with thrombin (T8021 ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: Eluted peptides were fractionated using a Superdex 30 Increase 3.2/300 column (GE Healthcare) at a flow rate of 10 µl/min using 30 % (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using a Superdex Peptide 10/300 GL gel-filtration column (GE Healthcare).